The study is conducted to investigate the effect of volatile oil extracted from the yellow peel of C. grandis fruits (Sindi) on growth of three bacterial species isolated from food samples , as well as A. flavus , and to check the possibility of using the volatile oils of this plant as a food preservative . Results showed a variation in the sensitivity of the bacterial isolates against the volatile oil which showed inhibitory effect on the growth of S. enteritidis and S. aureus with the increasing concentration of the volatile oils used in this studys , MIC for both bacteria was (0.12 , 1)% (v:v) respectively. Whereas , P. aeruginosa showed complete resistance to all treating concentrations used . Results of treating spore suspension of A. flavus with different concentrations of volatile oil showed also complete inhibition of germination at concentrations (0.12 , 0.25 , 0.5 , 1)%. Whereas lower concentrations (0.03 , 0.06)% delayed spore germination for (11) and (17) hrs after incubation respectively compared to the control treatment in which spores germination was within (6) hrs after incubation . Different concentrations of the volatile oil also affected the radial growth rate of A. flavus was colonies with the increasing concentrations . A complete inhibition of growth occurred at concentrations (0.12 , 0.25 , 0.5 , 1)% , Whereas lower concentrations showed a reduction of colony diameters with the increasing concentration
The field experiment was conducted at botanical garden of Department of Biology, College of Education for Pure Science/ Ibn-Al-Haitham, University of Baghdad to study the effect of Lepidium sativum leaves extract and pumpkin seeds Cucurbita pepo in two concentrations 10% and 20% on physiological characters ofPimpinella anisum. The results showed that in either concentration plant heights, dry weight, biological yield, economic yield, weight of 100 fruits, harvest index, absolute growth rate (AGR) were all increased and percentage of oil in fruits as compared with control plants
The experiment was conducted under shading (with the aid of Saran) condition on a nursery managed by the Baghdad Mayoralty during the season of 2014-2015 to study the effect of composed sheep manure extract on the growth and leaf nutrients content of tomato seedlings var. Wijdan. The experiment was composed of 6 treatments included the extract of sheep manure by hot (425C)and lmbient(205C) temperature water .The extract was diluted to the half by water and foliar applied to seedlings (multible application) or to the soil . Treatments also included the application of NPK chemical fertilizers as recommended and a control treatment through applying distilled water as foliar .The experiment was designed according to the randomized compl
... Show MoreTen isolates belong to the Staphylococcus bacteria from different clinical swabs were taken from patients in Ibn al-Nafis Hospital and Central Public Health laboratory, according to many morphological and biochemical tests that used to identify bacterial species S. aureus, the results showed that 8 isolates when investigated their ability to produce a slime layer using Congo red agar method the results showed that SA5 isolate was the best compared to other isolates through change the color of colonies to the pink and Congo red agar -colored Black.
When examining the inhibitory effect of grapefruit extracts in the growth of isolated bacteria SA5 S.aureus, results showed that the aqueous extract of the seeds at different concentrations
Swabs from two hospitals at Baghdad city were collected from the period 28-October-2013 to 31-March-2014, to found out the prevalence and distribution of fungi. These isolates were collected from hospitals' environment and most of the items used for hospital work in order to detect the nosocomial pathogens persistent. All fungal isolates were identified depending on the morphological and microscopic examinations as well as biochemical tests. Out of 220 environmental samples, 146 fungal isolates was obtained, Aspergillus flavus was more frequency and occurrence 23.29% and 15.45% respectively, followed Aspergillus niger was 15.57% and 10.45% then Penecillium spp. was 13.27% and 8.64%. In Al -Yarmouk Teaching Hospital more places contaminat
... Show MoreThe quantitative determination of the estrogen E2, Progestron P4 & follicle stimulating hormones FSH in the serums of Albino mice used in the experiments & treated by crude alcoholic extracted 95% of the seeds & leaves of Apium graveolens was a significant increase in the concentration of estrogen of different kinds of the extracted in comparison with the values of the coefficient of the control group, that is the study recorded a significant increase of the concentration of estrogen; it was the highest in the mice which was administrated by the methanolic extracted 95%, then it was less in the mouse which was administrated by the ethanolic extracted., and it was the least in the mice which were administrated by the crude ethanolic-methano
... Show MoreStaphylococcus Sp.is the most common type of bacteria found in contamination place, we design this
study to compare the contamination accident between two hospitals in Baghdad.One of them isthe Burns
Specialist Hospital in the Medical CityinRusafa and another one is Al-Karama Hospital in Karkh. The
samples were collected fromOperativeWard No1 (OW1), Operative Ward No2 (OW2), Consulting Pharmacy
(CP), Emergency Room (ER), Reception Room (RR), Women's Ward (WW) and Men's Ward (MW).The
samples were taken from inside each clinical unit, surfaces, food, and air. The results showed that the
number of samples containing Staphylococcus sp. bacteria is 81, including 45 belonging to Al-Karama Burns
Ward Ho
Klebseilla pneumoniae possesses many virulence factors and survival strategies to persist and overcome host defenses; one of these strategies is biofilm formation. Therefore, the aims of this study was to determine the antibacterial and antibiofilm effect of Rosmarinus officinelis L. essential oil (EO) and its effect on the genes encoding of fimbrial adhesions. The antimicrobial activity was investigated by MIC. The ability to form biofilm as well as inhibition of initial cell attachment and biofilm formation was performed. PCR was carried out to detect fimH-1 and mrkD genes of type 1 and type 3 fimbrial adhesions at different time of incubation. The study revealed that MIC value of EO was 104 μg/ml on 24 (83%) of isolates, 93% of them
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