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jih-1081
Determine the Biofilm Formed by Using ELISA Technology for Gram-Negative Bacteria Isolated from Wounds and Burns Infections, and the Study of the Production of the Biofilm Molecularly.
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    This study was designed to investigate the capability of gram-negative bacteria that isolated form wound and burn infection to production of Biofilm which included (32) isolates, which have multi – drug resistant to antibiotics. The isolates included (10) Pseudomonas aeruginosa, (9) Klebsiella pneumoniae, (6) Escherichia coli, (5) Proteus mirabilis and (2) Enterobacter cloacae. The method used method links the crystal violet with biofilm and reading by ELISA which was adopted on the values of optical density of violets that linked to the mass of biofilm at the wavelength of (620) nm, the test results showed variation of biofilm composition for all bacterial species depending on the optical density value while the most production of biofilm was product form bacterial isolated from diabetes patients wound. Agarose gal electrophoreses results show that all isolate have a different plasmid profile according to the (size and position), it was also that temperature used as a curing factor causes a high frequency loss the plasmid from all isolates and according to this reason the yield was different after the curing that maybe refers to the role of both plasmid and chromosome in regulating this phenomena.
 

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Publication Date
Mon Jan 15 2018
Journal Name
Iraqi Journal Of Laser
Effect of 410 nm Diode Laser Irradiation on the Growth of Burn Wounds-associated Bacteria, Pseudomonas Aeruginosa and Staphylococcus Aureus
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The effect of 410nm with 100 mW output power and one centimetre spot size (0.128 W/cm2 power density) Diode laser irradiation at different exposure times on the growth of Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus was evaluated. Seventy swap samples were collected from burn and infected wounds of 35 patients admitted to the burn-wound unit in Al-Yarmouk Teaching Hospital in Baghdad during the period from December 2014 to February 2015. These bacteria were isolated and identified depending on their growth on selective media, cultural characteristics, Gram stain morphology and biochemical tests and finally were confirmed by Vitek 2 compact system test .Susceptibility of bacterial isolates to 15antibiotics

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Publication Date
Tue Jan 01 2019
Journal Name
J. Pharm. Sci. & Res
Production of peroxidase from Providencia spp. bacteria
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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Use special Erwa to determine the number of live bacteria in different models
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Used in the study especially calibrated Erwa to determine the number of neighborhood or the Alayoshi number of bacteria in the count modeling and casting method dishes in addition to using the drop method yielded significant results for a match between the methods used ..

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Publication Date
Wed May 01 2019
Journal Name
Environmental Technology & Innovation
Biomineralization based remediation of cadmium and nickel contaminated wastewater by ureolytic bacteria isolated from barn horses soil
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Publication Date
Wed Mar 08 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Extraction and Estimation of β-carotene Pigment from Some Species of The Genus Rhodotorula and Study The Ability to Increas Their Production By Mutagenesis
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Beta-carotene pigment was extracted from 6 strains collected from different sources related to some species of the genus Rhodotorula sp. The maximum productivity was in the strain Rhodotorula  mucilaginosa BA61 with amount 10.25 gm/l. The minimum productivity was from the strain R. minuta BA78 with amount 5.39 gm/l. The effects of the chemical mutagen (MNNG) and the physical mutagen (UVC) on the viability of the strains was studied. The results revealed that the chemical mutagen (MNNG) with the concentration 0.2 mg/ml has the clear effect on the viability of the strains , which killing percentage reached to 65.91% in the strain R. minuta BA78. Results of the study of mutagenesis with UVC showed that increase in killing percentage fo

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Publication Date
Thu Jul 01 2021
Journal Name
Iraqi Journal Of Science
The Efficiency of Apple Vinegar as A Solvent in Comparison to Water and Ethanol for The Extraction of Some Plants used Against Candida Spp. Biofilm Formation
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Apple vinegar has many uses that include burn and wound healing and as an antimicrobial agent against different microorganisms, but not as a solvent. Therefore, this study aimed to use commercial apple vinegar as solvent to the plants of  roselle (Hibiscus sabdariffa), green tea (Camellia sinensis), and clove (Syzygium aromaticum). The effects of apple-vinegar extracts of these plants were compared with those of aqueous and ethanolic extracts against biofilm formation by Candida genus. Clove vinegar extract demonstrated antibiofilm activity against C. albicans, alone (2.4907± 0.382) or in combination with the antifungal agents fluconazole (1.689±0.33), nystati

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Publication Date
Tue Jan 30 2018
Journal Name
Iraqi Journal Of Science
Determine the Radon Gas Level Using the GIS Technique for Baghdad City
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In this work, radon concentrations in the polluted environment were measured within the Baghdad sample surface soil conservation, and this was done using a RAD-7 mobile detector. The work consists of four parts:
The first part includes calculating the latitude and longitude of each point of the study area using the Global Positioning System (GPS). The second part of which includes Determination of 222Rn gas concentrations in surface soil samples. In the northeast part of Baghdad, the highest concentration of radon was found in Al-
Shaab area (3.11 ±175.33 Bq / m3), while the lowest gas concentration in AL Gzeera area (6.67±73.00 Bq/ m3), In the northwest part of Baghdad, the highest concentratio

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Publication Date
Sun Sep 05 2021
Journal Name
Journal Of Arab Statistical Union (jasu)
Using Quality Control and 6-Sigma to Determine The Quality of The Treated Wastewater Discharged From Some Water Purification Plants
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Publication Date
Tue Feb 28 2023
Journal Name
Iraqi Journal Of Science
The Effectiveness of using the Biofilter made from Bidara (Sidr) to reduce the negative effects of Mice Cigarette Smoke
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       This study aims to determine the effect of exposure to cigarette smoke with a biofilter of bidara leaf powder on the histology of the liver, lungs and blood viscosity of mice. This test was carried out for 28 days by giving cigarette smoke with a biofilter of bidara leaf powder and observing the histology of the liver, lungs and blood viscosity. The results showed that exposure to cigarette smoke with a biofilter of bidara leaf powder was very effective in reducing damage to the liver and lungs of mice, and also very effective in reducing blood viscosity as in normal mice blood viscosity. The use of Bidara leaf powder biofilter can affect the histology of the liver, lungs and blood viscosity in mice. Bidara leaf powder biofi

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Correlation between virulence factor and biofilm formation in Proteus spp.
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Urinary Tract Infection is an infection that caused by the members of the genus
Proteus that depends mainly on the availability of virulence factors ;Various
virulence factors including biofilm, swarming migration , polysaccharide
,heamolysin,protease, DNase, urease production weredetermined for 45Proteus
isolates that obtained from clinical specimens of Urinry Tract Infection patient .
The distribution of virulence factors was showed variation among the testedisolates
and strain specific in most cases. All Proteus isolates showed 45 (100%)biofilm ,
polysaccharide andSwarming capabilities with different extents. High
ureaseproduction was demonstrated in most isolates 40 (88.8%);In addition, they
were abling to

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