This study focused on extracting the outer membrane nanovesicles (OMVs) from Escherichia coli BE2 (EC- OMVs) by ultracentrifugation, and the yield was 2.3mg/ml. This was followed by purification with gel filtration chromatography using Sephadex G-150, which was 2mg/ml. The morphology and size of purified EC-OMVs were confirmed by transmission electron microscopy (TEM) at 40-200 nm. The nature of functional groups in the vesicle vesicle was determined by Fourier transforms infrared spectroscopy (FT-IR) analysis. The antitumor activity of EC-OMVs was conducted in vitro by MTT assay in human ovarian (OV33) cancer cell line at 24,48 and 96hrs. The cytotoxicity test showed high susceptibility to the vesicles in ovarian compared to normal cells with IC50 of 7.169 µg/ml at 96hrs of incubation. The current strategy is an effective and simple method to obtain EC-OMVs. Keywords: ovarian cancer, lung cancer, antitumor, breast cancer, nanovesicle, MTT assay.
The inhibition effect of crude juice of green and black olive on cancer cell line (RD) in vitro has been studied by depending on micro titration system . Eleven different concentration starting from (916-960) mg/ml of crude juice respectively ,for three periods of exposure(24-48-72)hours. The resulted showed that the inhibition effect dependent on type of olive fruit juice ,concentration of dose ,time of exposure and the high concentration of both type of olive juice increased the growth of cell line while other concentration caused decrease in different rates ,moreover the black juice was more effective than green and 48 hours' time exposure was the best for inhibition.
There are many neutron sources in the universe that play an important role in the stellar slow neutron capture (s-process) nucleosynthesis. Fluorine-19 is a cosmically rare isotope that is generated in a series of reactions. The aim in this paper is to perform theoretical calculations to test the variance of neutron intensity generated within stellar conditions, especially in Asymptotic Giant Branch (AGB) stars, on the production of 19F isotope. EMPIRE II program has been utilized with the aid of many Matlab programs, and experimental comparisons have been made with NACRE II and Reaclib libraries. The results has shown that the high abundances of reactant nuclei responsible for ultimately generating
... Show Moreان الازمة الصحية العالمية الناشئة عن انتشار فيروس كورونا (والذي وصفته منظمة الصحة العالمية في 30/كانون الثاني/2020 بانه حالة طواريء صحية عامة مُثيرة للقلق الدولي، ثم عادت لتُسبغ عليه وصف الجائحة pandemic في 11/آذار/2020) وما أتخذته السطات الحكومية في دول العالم المختلفة من تدابير وأجراءات متفاوته (سواء بمستواها الاعلى من فرض حظر التجوال الشامل او ما اطلق عليه عالميا بالحجر المنزلي وبالتالي تعطيل كافة القطاع
... Show MoreThe current study aims to determine the extent of SOMO's interest in governance and whether this interest is sufficient to be reflected in enhancing its organizational reputation, and the field of research was in the oil marketing company SOMO. (109), (105) questionnaires were distributed, (94) forms and (11) forms were not retrieved, and the valid questionnaires had reached (91). The analytical and descriptive approach was used for the study, and the current research has found a positive impact of government on the reputation of the organization, and this research demonstrated the existence of the proposed relationship and the impact between governance and the reputation of the organization.
Introduction The pathophysiology of appendicitis likely stems from obstruction of the appendiceal orifice leading to an increase in intraluminal and intramural pressure, resulting in small vessel occlusion and lymphatic stasis. Organ supplied by an end artery, such as the appendix, are more prone to the deleterious effects of arterial occlusion. The continuous cigarette smoking might be associated with a greater risk for developing atherosclerosis.
Objectives The aim is to evaluate the effect of cigarette smoking on the incidence of complications of acute appendicitis.
Methods Patients suffered from symptoms and signs suggesting a
This research has studied the effect of addition glass fibers (woven and chopped)
and Zirconium oxide Nano-particles (ZrO2) with different weight percent to the
conventional poly (methyl methacrylate) (PMMA). The prepared Nano-crystalline
ZrO2 powder with particle size of about 95nm was syntheses directly by sol-gel
method. The gel dried at 100oC for 1 hour and annelid at 400oC for 3 hours.
The conventional acrylic resin prepared with 2:1 powder to liquid ratio to prepare
pure sample, composite samples prepared by reinforcing PMMA with woven or
chopped glass fiber (8, 12) wt.%, and reinforcing by (1,2,3) wt.% of prepared ZrO2
Nano-powder.
The structural tests include: (XRD, AFM, and FTIR). The crystallized phas
The research deals with the concept of rationalization of consumption, which is legitimately legitimized, by indicating the permissibility of consumption and its limits in Islam, the legitimate aspects of spending and its controls, and the concept of development in the Islamic economy. The researcher followed the descriptive method by presenting the legal texts and jurisprudential opinions and linking them to contemporary economic problems.The research has reached several conclusions, the most important of which is that the goal of Islam in relation to consumption is to inform the Muslim of the amount of economic maturity, and the truth of alms is good in money and religion. It is necessary for achieving maturity that both means
... Show MoreThe present work aims to study the removal of dyes from wastewater by reverse osmosis process. Two dyes were used direct blue 6, and direct yellow. Experiments were performed with feed concentration (75 – 450 ppm), operation temperature (30 – 50 oC) and time (0.2 – 2.0 hr). The membrane used is thin film composite membrane (TFC). It was found that modal permeate concentration decreases with increasing feed concentration and time operating, while permeate concentration increases with increasing feed temperature. Also it was found that product rate increase with increasing temperature, but it decrease with increasing feed concentration and time. The concentration of reject solution showed an increase with increasing feed concentratio
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