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: Partial purification of phosphoenolpyruvate carboxykinase (PEPCK) from type 2 diabetic patients sera take
place using some purification steps such as participation with ammonium sulphate (55-80%) and filtered through dialysis,
then ion exchange chromatography by DEAE sepharose anion column, gel filtration chromatography by sephadex G-100
column. In ion exchange step, there are four peak are obtained, the highest enzyme activity obtained by (0.4 M Nacl) with
purification fold (2.18), yield (44.3) of enzyme and specific activity (13.5) mg/ng, which obtained a single peak by gel filtration
chromatography, the degree of purification (5.34) fold, yield of enzyme (20%) with specific activity (33.109mg/ng). The purified
enzyme had an optimum tem
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