Background: One common undesirable side effect of orthodontic treatment with fixed appliances is the development of incipient caries lesions around brackets, particularly in patients with poor oral hygiene. Different methods have been used to prevent demineralization; the recent effort to improve the resistance against the demineralization is by the application of lasers. Materials and method: Thirty human premolars extracted for orthodontic purposes were used to test the effect of two energy level of ER-YAG laser on enamel resistance to demineralization. The brackets were bonded on the teeth and all the labial surface excluding 2 mm area gingival to the brackets were painted with acid resistance varnish. Three groups were generated. The first group was the control group (A), with no treatment was performed. In group II (B)and groups III (C); teeth were irradiated by ER-YAG laser of 200, 60 mj energy respectively. All the teeth were individually subjected to acid challenge cycle for 30 days. After debonding longitudinal sections were taken and examined under stereomicroscope. The enamel demineralization evaluation was done by taking the average of three depths at the centre of the artificial lesion. Also the enamel surface was classified by an experienced investigator according to acid etch pattern. Comparisons of the average depth values of the groups were performed with ANOVA and LSD tests. The statistical significance level was set at p ≤ 0.05. Results: The results revealed that average lesion depth was significantly deeper at the control group than the laser groups, and its significantly deeper in group (B) 200 mj than in group (C) 60 mj, enamel surfaces showed deeper pits and craters than in control group. Conclusions: the decrease in artificial caries lesion depth associated with use of the two laser energy level support the ER-YAG laser as a tool to increase enamel resistance to demineralization and white spot lesion prevention. Key words: Demineralization, ER-YAG, laser.