Background: Cluster of differentiation 14 (CD14) is a serum/cell surface glycoprotein; and it is a pattern recognition receptor. CD14 expressed on the surface of various cells, or it found soluble in saliva and other body fluids. It has been proposed that soluble CD14 (sCD14) may play a protective role by controlling Gram negative bacterial infections through its capacity to bind lipopolysaccharide. This study was conducted to assess the level of soluble CD14 in saliva of patients with different periodontal diseases and healthy subjects and determine its correlation with clinical periodontal parameters. Materials & Methods: A total of 80 subjects, age ranged (25-50) years old, divided into three main groups, group ? consisted of 45 chronic periodontitis patients, group ?? consisted of 20 gingivitis patients, lastly group ??? comprised 15 apparently- healthy volunteers. Unstimulated whole saliva samples were collected to determine levels of soluble CD14 in saliva by enzyme-linked immune–sorbent assay (ELISA). Clinical periodontal parameters were recorded at four sites per tooth including plaque index, gingival index, bleeding on probing, probing pocket depth and clinical attachment level. Results: A highly significant difference (P<0.01) was found for salivary sCD14 levels among the three groups, also it was greater in chronic periodontitis group than those detected for gingivitis and healthy controls with a highly significant difference (P<0.01). Furthermore, Spearman’s correlation analysis showed statistically highly significant strong correlations (P < 0.05) between salivary sCD14 levels and each of (probing pocket depth, clinical attachment level). And non-significant correlation between salivary sCD14 level with plaque, gingival & bleeding on probing indices. Conclusion: The findings of the present study reemphasize the importance of whole saliva as sampling method in terms of immunological purposes in periodontal disease and suggest that the elevated sCD14 concentration may be one of the host-response components associated with the clinical manifestations of periodontal disease.
The current study was carried out to investigate the correlation of gene expressions of ADA1 and ADA2 genes with the development of autoimmune thyroid disease (AITD) in a sample of Iraqi females. One hundred patients with AITD and 80 controls were included. Quantitative real time polymerase chain reaction (qRT–PCR) was utilized for investigation of ADA1 and ADA2 gene expression among patients and controls. The correlation of age and body mass index (BMI) with AITD occurrence comparing with controls was studied. Based on the results of this study, there is high expression level of ADA1 and ADA2 genes in patients compared with healthy controls; also, the gene expression fold (2-ΔΔCT) of ADA1 and ADA2 among AITD patients was recorded and a
... Show MoreIntroduction: Trichophyton indotineae has emerged globally as a highly contagious, terbinafine-resistant dermatophyte causing treatment-refractory superficial mycoses, posing a significant public health threat.This study evaluated the epidemiology of dermatophytosis in Babylon Province, Iraq, and performed molecular identification and phylogenetic analysis of local clinical isolates. Methods: Clinical specimens from 258 patients aged 1 to 71 years were analyzed. Following phenotypic characterization, genomic DNA was extracted from three representative isolates. Polymerase chain reaction amplified the internal transcribed spacer region, followed by DNA sequencing and neighbor-joining phylogenetic analysis. Results: Tinea corporis was
... Show MoreBackground: The surface properties of the titanium alloy plays a significant role in the bond of the dental implant with living bone and modification of the implant surface could enhance osseointegration. This study was aimed to investigate the effect of different durations of heat treatment on the surface properties of titanium alloy for dental implants. Materials and methods: Twenty disks of (Ti-6Al-4V) alloy were prepared. The sample was divided into four test groups to study the effect of different duration of heat treatment to the surface topography; surface chemistry, titanium oxide layer thickness, blood contact angle, & blood drop diameter of titanium alloy samples were investigated to evaluate the effect of different durations of
... Show MoreBackground: Complete analysis of facial profile should also include an evaluation of soft tissue morphology. Materials and Method:The sample consisted of 90 Iraqi adults (45 males and 45 females) aged 18-25 years from Baghdad city divided into 3 groups according to the ANB angle with 30 subjects in each group (15 males and 15 females) for class I, II and III. Lateral cephalometric radiograph was taken for each subject and 8 angular and 5 linear measurements were identified and determined, t-test, ANOVA and LSD test were used to compare between both genders and between different classes. Results:Showed that females had greater angular measurements and smaller linear measurements with more lip prominence than males in all classes, there was m
... Show MoreBackground: with the advent of new postmaterial in dentistry, it has become important to measure fitness of post restoration along the horizontal plane of the root space.This study aimed to measure and compare, the cement film thickness of conventional zinc phosphate cement in micrometer between the post and root dentin along horizontal plane at different post space regions (coronal, middle and apical) of four types of posts, by using stereomicroscopy. Material and methods: Thirty-two extracted human maxillary canines, mandibular canines and maxillary central incisors (n=32) were instrumented with ProTaper system files (hand use) and obturated with gutta-percha for ProTaper and AH26® root canal sealer. After 24hrs of incubation at 37ºC, p
... Show MoreThis work presents experimental research using draped prestressed steel strands to improve the load-carrying capacity of prestressed concrete non-prismatic beams with multiple openings of various designs. The short-term deflection of non-prismatic prestressed concrete beams (NPCBs) flexural members under static loading were used to evaluate this improvement. Six simply supported (NPCBs) beams, five beams with openings, and one solid specimen used as a reference beam were all tested as part of the experiment. All of the beams were subjected to a monotonic midpoint load test. The configuration of the opening (quadrilateral or circular), as well as the depth of the chords, were the varia
This work presents experimental research using draped prestressed steel strands to improve the load-carrying capacity of prestressed concrete non-prismatic beams with multiple openings of various designs. The short-term deflection of non-prismatic prestressed concrete beams (NPCBs) flexural members under static loading were used to evaluate this improvement. Six simply supported (NPCBs) beams, five beams with openings, and one solid specimen used as a reference beam were all tested as part of the experiment. All of the beams were subjected to a monotonic midpoint load test. The configuration of the opening (quadrilateral or circular), as well as the depth of the chords, were the varia
Long-term use of sulfonylureas including chlorpropamide, is known to potentiate the antidiuretic action of arginine vasopressin (AVP), predisposing to hyponatremia.The present study was designed to evaluate the effect of long term use of glibenclamide on serum and urinary levels of sodium and potassium in Type 2 DM patients in Iraqi DM centers. Ninety eight patients with Type 2 DM who were maintained on different doses of glibenclamide for at least 1 year, attending the centre for Diabetes and Endocrinology in Al-Rusafa, Baghdad, were enrolled in the study, in addition to 15 normal healthy subjects. Patients were allocated into three groups according to the dose of glibenc
... Show MoreBackground: Neural tube defects (NTDs) are said to be inherited in a multifactorial fashion, i.e. genetic-environmental interaction. Maternal nutritional deficiencies had long been reported to cause NTDs, especially folate deficiency during early pregnancy. More attention had been paid to the exact mechanism by which this deficiency state causes these defects in the developing embryo. The most significant of all researches was that connecting reduced folate and increased homocysteine level in maternal serum on one hand and the risk of developing a NTD baby on the other hand. Objectives : to determine the significance of homocysteine level in Iraqi mothers who gave birth to babies with NTDs as compared to normal controls. Patients, Materials
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