This Study aimed to studying the effect of Volatile oil extracted from the leaves of Myrtus communis on the growth and activities of the following types of bacteria: Staphylococcus aureus, Streptococcus pyogenes, Klebsilla pneumoniae, Pseudomonas aeruginosa, and the yeast Candida albicans. The results showed an inhibitory effect of the oil on both the growth and activity of the tested microbes. This was reflected by the minimum inhibitory concentration (MIC) of Staphylococcus aureus, Streptococcus pyogenes, Klebsilla pneumoniae, Pseudomonas aeruginosa which was: (2.5, 1.25, and 2.5,5 % respectively), and the yeast (5) %. Also, the Minimum bactericidal concentration (MBC) to the bacteria mentioned above was (5, 2.5,5,10 % respectivel

Background: The aim of this study was to evaluate the push-out bond strength of four different obturation materials to intraradicular dentin and to determine the failure mode. Materials and method: forty straight palatal roots of the maxillary first molars teeth were used in this study, the roots were instrumented using crown down technique and rotary EndoSequence system, the roots were randomly divided into four groups according to the materials used for obturation (n=10).Group (1): AH Plus sealer and gutta-percha. Group (2): Activ GP glass ionomer sealer and Activ GP gutta-percha (Activ GP system). Group (3): Bioceramic sealer and Bioceramic gutta-percha. Group (4): GuttaFlow2 sealer and gutta-percha. For all groups single cone obturatio

Enterococci are usually encountered and predominate in oral infections, especially those associated with dental root canal infections of necrotic pulp and periodontitis. This study aimed to detect and identify Enterococcus faecium isolated from infected root canals, using polymerase chain reaction ( PCR). Thirty samples were collected from patients with  necrotic pulp, infected root canals, and endodontic treatment failure, attending the Conservative Treatment Department, College of Dentistry, Mosul University, Dental Teaching Hospital. The samples were obtained by inserting sterile paper points into the root canals and transferred in brain heart infusion broth vials to be  inoculated in a selective M-Enterococcus Agar Base . T

Silver nanoparticles synthesized from aqueoes extract for mushroom pleurotus ostreatus. Silver nanoparticles were showing good antibacterial activity. The antibacterial activity conducted against different pathogenic bacteria such as Serratia marcescens, Pseudomonas fluorescens, Listeria, Methicillin-resistant Staphylococcus aureus (MARSA), Proteus mirabilis, Proteus vulgaris and Klebsiella pneumoniae. The maximum inhibition zone was observed against S. marcesance. However, the lowest inhibition zone was found against P. fluorescens.

Biofilm formation (BF) is one of the most important virulence factors of
Candida spp. The aim of this study was to detect the prevalence of genes
responsible in biofilm formation of C. albicans by conventional PCR technique.
Among 49 vaginal specimens (VC), C. albicans was the most predominant species
in percentage 22/49 (45%) and 27(55%) were non albicans. Out of 47 oral
specimens (OS), 22/47(47%) were C. albicans, whereas 25(53%) were non albicans.
At the present study; all C. albicans were biofilm producers with variable strength,
out of 44 BF producers, 18 (40.9%) were low biofilm (LBF) with significant
differences (P<0.05) between HVS and OS, 25 (56.8%) moderate or high biofilm
(HBF) and just one isolat

Three beach soils in Lagos, Nigeria were screened for the presence of antibiotic producing fungi against 8 test pathogenic bacteria & fungi. The physiochemical parameters of the soils were determined following standard procedures. Soil plate dilution method was employed for isolation of marine fungi and they were identified based on cultural and microscopic characteristics. Primary screening of isolated fungi for antibiotic potential was determined by perpendicular streak method against known pathogenic test organisms (Escherichia coli, Saphylococcus aureus, Klebsiella pnuemoniae, Pseudomonas aeruginosa, Candida albicans, Saccharomyces cerevisiae,Aspergillus niger and Aspergillus fumigatus). Further sreening of the product o

The utilization of metal oxide nanoparticles, especially zinc oxide, is of a great importance in the medical field because of its physical and chemical properties as well as its antimicrobial potential effects. In our study, the ZnO nanoparticles were synthesized by the precipitation method where pH=14. ZnO nanoparticles were characterized by ultraviolet–visible spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and atomic force microscope (AFM). Antifungal activity of the ZnO was tested against candida albicans. The results showed that C. albicans (15 samples) became resistant to the fungal activity after testing its sensitivity to several types of fungal antibiotics. UV-vis spectroscopy, XRD, TEM and A

  This study was conducted to determine the effect of different concentrations of   ethanol  extract of propolis against two  fungi Botrytis cinerea, Altrnaria sp.      The most important chemical constituents of propolis diagnosed by the Infra Red Spectroscopy, were the  Flavonoids  specificaly.       Results showed a high antifungal activity of ethanol  extract of propolis, evidently with a high concentration  of extract (5) mg\ml, which completely inhibited the radial growth on both  solid and liquid media (PDA &PD Broth ) of both fungi .         We observed that , ethanol  extract p

This study focuses on diagnosis of Candida species causing Vulvovaginal Candidiasis using phenotype and genotype analyzing methods, and frequencies of candida species also using Vulvovaginal Candidiasis patients. 130 samples (100 from patients and 30 from non infected women) were collected and cultured on biological media. Identifying the yeasts, initially some phenotypic experiments were carried out such as germ tube, from motion of pseudohyphae and clamydospores in CMA+TW80 medium, API20 candida and CHROMagar Candida. Genomic DNA of all species were extracted and analyzed with PCR and subsequent Polymerase Chain Reaction - Restriction Fragments Length Polymorphism (PCR-RFLP) methods. Frequency of C. albicans, C. krusei, C. tropicalis , C.