Background: White spot lesions (WSLs) are subsurface enamel demineralization manifested as white opacities which had an esthetic problem. The purpose of this in-vitro study was to evaluate the lesion depth improvement of WSLs following application of fluoride varnish, tooth mousse and resin infiltration (ICON). Materials and methods: Artificial WSLs were created on 120 premolar teeth using demineralization solution with pH (4-4.5). Samples randomly allocated into four groups; fluoride varnish, tooth mousse, ICON and untreated group. Groups were discolored in Cola and orange juice for 24 hours. Teeth were ground sectioned by longitudinal cutting then these sections examined and photographed under stereomicroscope at 12X magnification then analyzed for lesion depth measurements using Image Pro Plus computer software to record the average lesion depth (μm). Results: The mean values of lesion depth for WSLs groups in DDW, Cola and orange juice increased after formation of WSLs then decreased with the fluoride varnish, tooth mousse and ICON. There was a statistically significant difference between the WSLs, fluoride and Mousse group in DDW and Cola, while there was no statistically significant effect of ICON on WSL in DDW (p=0.341)and Cola (p=0.210). Conclusions: Formation of WSLs is associated with significant lesion depth changes and the use of fluoride varnish, tooth mousse and ICON could return minerals or resin to subsurface enamel of WSLs and improve lesion depth in different soft drinks.