Background: Tooth eruption is a more general process, however, which includes certain posteruptive tooth movements. There are two fundamental requirements for both tooth eruption to occur: (1) Require soft tissue, intervening between tooth structure and alveolar bone, which plays an important role in regulating the remodeling of adjacent tissues. (2) Require bone turnover that is temporally and spatially regulated to facilitate specific translocations of teeth through alveolar bone These amniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for human application and recently it used in many medical branches. CD34 is an endothelial marker that is extensively used in immunohistochemistry and most vascular endothelial cells. Expression of the stem cell antigen CD34 is a defining hallmark of hemopoietic stem cells and progenitors. This study aimed to study the expression of CD34 by dental cells involved in tooth eruption after administration of amniotic stem cell Materials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells in the anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice for each period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry. Results: Immunohistochemical result revealed positive expression of CD34 in pulp (Vascular, Paravascular), Mesenchymal cell and in the Dental sac of different groups. Coincidence test of expression marker CD34 in various studied group shows that Chorion application affected on CD34 expression in pulp while Amniotic fluid affected on dental sac. Conclusion Immunohistochemical study of expression marker CD34 in various studied groups show that chorion application affected on CD34 in pulp .While amniotic fluid affected on dental follicle.
Background:
Angiogenesis plays a crucial role in tumorigensis; several reports have described a significant increase in microvessel density (MVD) in colorectal carcinogenesis There are
several methods to measure the angiogenesis in neoplasms, but immunohitochemistry seems to be the mainstay of all. This method enable us to measure the tumor microvessel densities highlighted by using antibodies directed against endothelial cell markers like CD31,CD34 or others; then assessment of MVD by manual count of the number of microvessels in what appears to be the most vascular area of the tumor(called the hot spot) using a protocol described by Weidner et al.Automated cellular imaging system is used to analyze
The present study aimed to isolate and diagnose mesenchymal stem cells derived from human bone that is the source generating cells that are the best types of treatment for tissue diseases.
Cells were isolated from the back bone of the human pelvis, separated using density gradual sedimentation method and then the cells were grown on the culture media RPMI-1640 \ 20% FBS.
To detect the purity of cells that have been isolated and have been transplanted immune use the method using CD44 (mesenchymal stem cells marker) CD43, a specific marker for hematopoietic cells Nestin, (the neurons private marker).
The present study has shown that mesenchymal cells that have been isolated and expanded in this experiment has reached up 99.7% for
In this review of literature, the light will be concentrated on the role of stem cells as an approach in periodontal regeneration.
Back ground: Although adult stem cells possess plasticity that permit differentiation along new lineages, production of exocrine and endocrine pancreatic cells and insulin-secreting beta-cells from adult non pancreatic stem cells has been considered controversial. We present that highly purified adult rat hepatic oval stem cells, which are capable of differentiation to hepatocytes and bile ducts epithelium, can also trans-differentiate into pancreatic exocrine-endocrine tissue, when homogenized hepatic tissue is implanted into subcutaneous tissue .
Methods and Materials: A total of 60 adult Swiss albino rats were divided into two groups . Group I , control group (30 animals) was injected with normal salin
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This work involves separating and studying the aminoacylase-1 (ACY1) of amniotic fluid from healthy pregnant, mainly one peak with higher activity has been isolated by DEAE-Cellulose ion exchange from the proteinous supernatant produced by deposition of proteins using ammonium sulfate (65%) after dialysis. The purification folds reaching to 19 folds also gave one protein peak when injected into the gel filtration column, a high ACY1 purity was obtained, with 38 folds of purification. It was found that the molecular weight of the isolated ACY1 was up to 46698 Dalton when using gel chromatography technique.The effect of ACY1 isolate was studied on rats with oxidative stress caused by lead acetate(LA) at 40 mg / kg body weight and compare
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The inflammatory reactions cause nasal polyposes (NPs), which contained the paranasal sinuses and the nasal mucous membrane. They consist of recurrent multiple masses originating in the paranasal sinuses then spread from the middle meatus to the nasals cavity, which leads to the nasal blockage that causes the restriction of airflow to the olfactory area. This study aims at clarifying the role of IL-12RB2 polymorphism by using PCR technology in nasal mucosal stem cells in nasal polyps of Iraqi patients and use it as a biomarker. Fifty-eight cases of this study are referred to as nasal surgery, which selected from Dept.of Otolaryngology, Baghdad City, Iraq from May 2013 to January 2014. They were grouped into Control group (022 samples
... Show MoreBackground: Astrocytoma, is heterogeneous tumor of the nervous system and studies on the virulence of these tumors reveal that their behavior is led by small population of cells which are the brain tumor stem cells (BTSCs) that drive the continuous proliferation and self-renewal. From the many markers that annotate BTSCs, are the CD133, and NESTIN.
Objectives: Using CD133, to immunolabel BTSCs niches in paraffin sections of astrocytoma then, extraction of these cells, to calculate fold expression of NESTIN gene across the grades by real time PCR.
Materials and methods: Paraffin blocks of four grades of primary astrocytoma have been selected from three age groups from Iraq
... Show MoreThe dynamic development of computer and software technology in recent years was accompanied by the expansion and widespread implementation of artificial intelligence (AI) based methods in many aspects of human life. A prominent field where rapid progress was observed are high‐throughput methods in biology that generate big amounts of data that need to be processed and analyzed. Therefore, AI methods are more and more applied in the biomedical field, among others for RNA‐protein binding sites prediction, DNA sequence function prediction, protein‐protein interaction prediction, or biomedical image classification. Stem cells are widely used in biomedical research, e.g., leukemia or other disease studies. Our proposed approach of
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