Background: Healing of a tooth extraction socket is a complex process involving tissue repair and regeneration. It involves chemotaxis of appropriate cells into the wound, Transformation of undifferentiated mesenchymal cells to osteoprogenitor cells, proliferation and differentiation of committed bone forming cells, extracellular matrix synthesis, mineralization of osteoid, maturation and remodeling of bone. These cellular events are precisely controlled and regulated by specific signaling molecules. Some of these like transforming growth factor beta (TGF-?), vascular endothelial growth factor (VEGF), bone morphogenetic proteins (BMP) and insulin like growth factors (IGF) are well conserved proteins involved in the initial response to injury and repair in soft and hard tissue. Materials and Methods: 48 rabbits weighting an average of (2.5 - 3 kg) were used in this experimental study, and divided into 3 groups as follows; group A ( contains 16 healthy rabbits regarded as control group ) , Group B ( contains 16 diabetic rabbits not received treatment ), group C ( contains 16 controlled diabetic rabbits received insulin as a treatment ), the lower incisor for each rabbits was extracted, after 2, 10, 20 and 30 days of healing periods after scarification , the socket was analysed by immunohistochemical (IHC) estimation of growth factors : TGFbeta-3 , VEGF , IGF-1R , BMP-4 . Results: IHC findings revealed high positive expression of TGFbeta-3, BMP-4 on fibroblasts, osteoproginetor cells, osteoblasts and osteocytes,high positive expression of VEGF on endothelial cells and high positive expression of IGF-1R on endothelial cells and moderate expression on osteoblasts. Conclusions: The inhibition of proliferation and migration of osteoblasts, or differentiation from progenitor cells, is implicated in the delay of teeth sockets healing. For this fact the results of the present study concluded that in the diabetic healing bone (rabbits of group B), the onset of cell proliferation and osteoblast differentiation were delayed and subsequently prolonged healing process when compared with the other groups (rabbits of group A,Cand D).
ABSTRACT : The restoration of bone continuity and bone union are complex processes and their success is determined by the effectiveness of osteosynthesis. The use of plants for healing purposes predates human history and forms the source of current modern medicine. This research was planned to study the histological and immunohisto-chemistry of osteocalcin to evaluate of effect of local application of lepidium sativum oilon healing of induced bone defect in rat tibia. In this study, fourty albino male rats, weighting (300-400) gram, aged (6-8) months, will be used under control conditions of temperature, drinking and food consumption. The animals will subject for a surgical operation of medial side of tibiae bone, in control group the bone
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Curcumin is a yellow pigment produced from the rhizomes of the Curcuma longa plant and a primary chemo preventive component of turmeric is used as a spice and food coloring ingredient. Curcumin has a large number of pharmacological activities, such as anticancer, anti-diabetic, antioxidant, anti-infectious, and anti-inflammatory properties.Investigation of the geno-protective effect of curcumin on methotrexate induces chromosomal aberrations of spleen and bone marrow cells. In this study, 32 mice were used and divided into four groups (eight mice at each group) as follows: Group1 (negative control): Dimethyl sulfoxide was given intraperitoneally to mice every day for ten days.Group2 (positive control): Mice were received a single do
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Genotoxic effects of crude bacteriocin extracted from Citrobacter freundii were detected on albino mice bone marrow cells in vivo, using micronucleus (MN) and comet assay. The mice were administered intraperitoneally with 37.5, 75, 150 and 300 mg/kg of the extract for 24 hours. C. freundii was isolated from patients suffering from urinary tract infections (UTI). The bacteriocin producing isolates were determined using cup assayand the most efficient bacteriocin producers were selected. Bacteriocin was extracted from the efficient isolates via the induction with Mitomycin-C (2 mg/ml). Bacteriocin activity (320 U/ml) was determined by well diffusion method, while the protein concentration (2900µg/ml) was estim
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Background: A substance that can affect DNA or chromosomes is defined as a genotoxin. DNA damage in a somatic cell may result in a somatic mutation (cancer). In contrast, damage to a germ cell (germline mutation) may result in a heritable changed characteristic.Omega-7 is a non-essential monounsaturated free fatty acid with anti-inflammatory, anti-obesity, and antidiabetic effects.
Objectives: Evaluation of the possible protective effects of omega seven against methotrexate genotoxicity.
Method: Two major equal groups were obtained from seventy mice, and five subgroups (each of seven) were created from these groups as follows: Group I received liquid paraff
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Chemotherapy drugs have a major role in fighting cancer but, that doesn’t mean they are clear
from side effect on human health. One of these drugs is vincristine this study, the
chemopreventive potency of the crude extract ofIn Eruca Sativa Mill Which have a good
reputation as antioxidant and anticancer against chromosomal aberration and suppressed
mitotic index in bonemarrow cells of male albino mice treated with 50µ1 of vincristine
intrapritoneally(ip) have been detected.
Results Showed a Significant increasing in the percentage of the mitotic index nearly to the
control group and a significant decrease in chromosomal aberration after (35) days of
treatment with 250 mg/kg crude extract of Erucas
Aims: This study was done to investigate the effect of low energy laser therapy on bone healing at the extraction site. Materials and methods:(24) male albino rats were exposed to the extraction procedure of the maxillary first molar on the first day of a seven day experiment and these animals were divided into two main groups; the control group and the laser group. The laser experiment involved using (Ga-As infrared diode laser) from optodent by directing the probe over the extraction site. The control group consisted of 4 rats, and the laser group was subdivided into 5 subgroups of 4 rats each. The laser dose was as follows: B1: a single dose of 5 minutes immediately after extraction.,
... Show MoreThe potential use of bone marrow stromal cells as a cellular therapy for chronic diseases relies on the ability of the cell to replicate extensively in vitro.For this reason the present study investigated the replication lifespan and examined the growth properties of albino rats mesenchymal stem cells(MSCS)in vitro. To establish an in vitro system for isolating and culturing the MSCs of albino rats and to provide research data for its further application,the bone marrow (BM)was collected from young male rats and separated by gradient centrifugation.Then, the mononuclear cells(MNCs) were retrieved from the buffy layer and cultured in Modified Eagle,s Medium (MEM) supplemented with 10%Fetal Calf Serum (FCS)and incubation
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Genetic material is the most important component of cells because it contains the genetic information; hence any disruption to the structure chromosome of cells could lead to very bad results. Genotoxicity use to evaluate the safety of any chemical compounds on genetic materials. Artificial food flavoring additive are chemical substances to produce specific placebo effects added to foods but impart specific flavor to it.
The present study evaluates the genotoxic effect of artificial food flavoring additive on structure of chromosomes at three different concentrations (50%, 100%and 150%) on both bone marrow cells and spleen cells in mice for fourteen successive days. It was found that artificial food flavoring addit
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The aim of the study was extraction of arial part of Euphorbia cyathophora constituents with methanol and evaluate its effect on mitotic index and total chromosomal aberration bone marrow cell and spleen cell in mice 200 gm of E. cyathophora fine powder was defatted then extracted by cold maceration 80% ethanol for seven days. The extract was filtered and dried in a rotary evaporator then the dried extract was suspended with water and consecutively extracted using chloroform, ethyl acetate for each. The aqueous layer was then mixed with 100ml methanol. These fractions are dried under reduced pressure to obtain the dry extract. Twenty-four Albino mice were used for the experiment. The animals were divided into four groups: Gr
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Fisetin is a plant flavonoid found in strawberries and other fruits and vegetables such as apples, persimmons, and onions. It has many pharmacological effects like anti-inflammatory, antioxidant, cardioprotective, neuroprotective, and anti-carcinogenicity which are attributed to its ability to reduce oxidative stress which considers the main reason for different disease conditions. Genotoxicity refers to the genetic material destruction within the cell which can be caused by different chemicals as well as radiation. The present study evaluates the effect of orally-administered fisetin daily for seven constitutive days on genotoxicity induced by cyclophosphamide in rats’ bone marrow and spleen cells. Results showed that fisetin exh
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