Background: Saliva is a specific bio-fluid with important biomarkers. Analyzing any alternation in these markers could give valuable information, in relation to oral health status parameters. The aim of this study was to investigate the level of α -amylase in unstimulated whole saliva of healthy, primary school children in relation to some oral health parameters. Materials and Methods: A questionnaires consisted of demography and medical histories of participants were filled by children families. Saliva samples were collected for 5- minutes between 9:00 -11:00 AM from 114 healthy students aged 6-13 years, divided into four age groups. Flow- rate, Plaque and Gingival Index were assessed and dentition status was investigated by DMFT/dmft using WHO criteria. Salivary amylase was analyzed in unite per litter, using quantitative colorimetric amylase determination at 585nm. Results: A significant positive correlation was found between age and salivary flow-rate, (r=0.362, P < 0.001). Salivary α-amylase concentration increased significantly with age (P< 0.001). For each one year there is an increase in age, amylase level is expected to increase by 5.2 U/L. A male gender is expected to reduce salivary α -amylase level by 10.6 U/L compared to female, however the effect was not significant. Gingival index was positively, although non-significantly associated with salivary α -amylase concentration. DMFT showed a significant weak positive linear correlation with salivary amylase level (r=0.309, P<0.001), while deciduous teeth decay experience and plaque index were significantly and negatively associated with salivary amylase. Conclusion: Results emphasize the importance of salivary amylase, as a non-invasive biomarker in regulating oral and dental health status in children.
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Background: The aims of the study were to evaluate the unclean/clean root canal surface areas with a histopathological cross section view of the root canal and the isthmus and to evaluate the efficiency of instrumentation to the isthmus using different rotary instrumentation techniques. Materials and Methods:The mesial roots of thirty human mandibular molars were divided into six groups, each group was composed of five roots (10 root canals)which prepared and irrigated as: Group one A: Protaper system to size F2 and hypodermic syringe, Group one B: Protaper system to size F2 and endoactivator system, Group two A:Wave One small then primary file and hypodermic syringe, Group two B:Wave One small then primary file and endoactivator system, Gr
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