The aim of this study is to synthesize an easy, non-toxic and eco-friendly method. Silver nanoparticles which were synthesized by leaf extract of mint were characterized by UV-Visible Spectroscopy which appears UVVisible spectrum of demonstrated a peak 448 nm corresponding to surface Plasmon resonance of silver nanoparticles, Fourier Transform Infrared Spectroscopy (FTIR); functional groups involved in the silver nanoparticles synthesis were identified, the presence of silver nanoparticles was confirmed by X-ray diffraction (XRD) and Atomic Force Microscope (AFM) analysis clearly illustrated that the shape of silver nanoparticles was spherical and the size of the silver nanoparticles has been measured as 55- 85 nm. Evaluation of its antimicrobial activity, the resulted showed that efficiency inhibitory activity against bacteria and fungi, silver nanoparticle showed a greater effect on Staphylococcus aureus at a concentration of 150 μg/ml which reached in diameter of the inhibition zone 18.5 mm. whereas in fungi, Candida utilisat a concentration of 150 μg/ml, which reached in diameter of the inhibition zone 18mm.
