Background: The effect of Helium Neon laser (He-Ne 632.8 nm) was reported to protect cells from damage. We studied lymphocyte cells pre irradiated with (UVC 260 nm) to induce DNA damage. Investigations were carried using gel electrophoresis and test for cell viability. It has also been reported that effect depends on incubation period after damage. The extent of damage to the cells depends on the period of irradiation with UVC also on its intensity.
Objective: In this work we studied the effect of UVC on DNA damage and cell survival
Also study of the effect of He-Ne laser on cell survival after all being pre irradiated with UVC light and its protective effect on DNA post UV damage.
Method: This study was conducted in pathology department post graduate laboratory - College of medicine-Baghdad University. The total number of samples was (147). Blood samples were collected from healthy donors came to the blood bank, the amount of blood drown varies from 5ml to 7ml in heparin tubes .The work was carried out during the period between November 2010 to August 2011. In this experiments examination of samples was carried out to test the radiation effect on cell viability by using trypan blue dye, the experiments were preformed after 1, 24 and 72 hours post UVC irradiation to test the repair development. In other experiments Gel electrophoresis were carried out on samples to study the effect of radiation on the DNA fragmentation.
Result: Results reveal a reduced DNA fragmentation appeared on gel electrophoresis experiments as the smear length is reduced significantly for both UV10 and UV20 , other results for cell viability tests revelled that He-Ne can increase survival of cells pre irradiated with UVC irradiation giving (66%, 57%, 70%( improvement in UV exposure for10 min and (59%,56%,59%) improvement for UV exposure for 20 min respectively .
Conclusion: The effect of the laser in the improvement of cell survival may be attributed to the induction of endogenous radioprotectore and probably enzymes induced by laser irradiation which may be either reduce the free radical by scavenging effect or by improved cell repair, we may conclude that He-Ne laser can protect cells from radiation damage
Background: Rheumatoid arthritis is an autoimmune disease that affects mainly the synovial membranes and articular structures and is characterized by chronic, systemic inflammation involving multiple joints.Being a synovial joint, the Temporomandibular Joint is subject to the same disorders affecting other synovial joints, including RA.Beside it was considered as a specific serological marker for diagnosing RA disease ,antibodies to cyclic citrullinated peptide have proven to be associated with joints destruction, though; it may play a potential role in the prediction of the disease severity. Materials and Methods: Sixty nine individuals (69) were enrolled in this study, forty nine (49) were patients diagnosed with Rheumatoid Arthritis, a
... Show MoreFusidic acid (FA) is a well-known pharmaceutical antibiotic used to treat dermal infections. This experiment aimed for developing a standardized HPLC protocol to determine the accurate concentration of fusidic acid in both non-ionic and cationic nano-emulsion based gels. For this purpose, a simple, precise, accurate approach was developed. A column with reversed-phase C18 (250 mm x 4.6 mm ID x 5 m) was utilized for the separation process. The main constituents of the HPLC mobile phase were composed of water: acetonitrile (1: 4); adjusted at pH 3.3. The flow rate was 1.0 mL/minute. The optimized wavelength was selected at 235 nm. This approach achieved strong linearity for alcoholic solutions of FA when loaded at a serial concentrati
... Show MoreThe relationship between the clinical singes and some of the hematological and biochemical values in 126 cases of local breed and Shammi breed goats (from both sexes) diagnosed as cases of malnutrition from 230 goats examined .The clinical examination include (beside the general inspection and case history) body temperature, respiratory and pulse rates, mucus membranes skin and coat. Examination of the blood samples included RBCs count, Hb concentration, PCV % and values of cupper, magnesium, phosphorus, and potassium. The results showed that the mean body temperature of these goats was lower than in normal goats, while the respiratory and pulse rates were higher .Examination of blood revealed decrease in the means of RBCs counts ,Hb con
... Show MoreThis study is considered the first effort of its kind in Iraq and in the Middle East towards studying the Marbled Teal Marmaronetta angustirostris that was carried on in Hor Al-Dalmaj, southern Iraq. The findings of this effort illustrate its importance as it paves the way for further study and observation for the bird and this important wetland itself. This study tackles the all possible aspects of the ecological and biological statuses of Marbled Teal (Threatened –Vulnerable bird species – IUCN Redlist) by means of field surveys and systematic monitoring that were conducted along the four seasons over the years 2013-2014 in one of the ecologically important and prominent and poorly-known wetlands in the
... Show MoreLaboratory model tests were performed to investigate the behavior of shallow and inclined skirted foundations placed on sandy soil with R.D%=30 and the extent of the impact of the positive and negative eccentric-inclined loading effect on them. To achieve the experimental tests, it was used a box of (600×600) mm cross-sectional and 600mm in height and a square footing of (50*50) mm and 10 mm in thickness attached to the skirt with Ds=0.5B and various an angle of (10°, 20°, 30°). The results showed that using skirts leads to a significant improvement in load-carrying capacity and decreased settlement. In addition, when the skirt angle increased, the ultimate load improved. Load-carrying capacity decreased with increasing eccentri
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