Background: The effect of Helium Neon laser (He-Ne 632.8 nm) was reported to protect cells from damage. We studied lymphocyte cells pre irradiated with (UVC 260 nm) to induce DNA damage. Investigations were carried using gel electrophoresis and test for cell viability. It has also been reported that effect depends on incubation period after damage. The extent of damage to the cells depends on the period of irradiation with UVC also on its intensity.
Objective: In this work we studied the effect of UVC on DNA damage and cell survival
Also study of the effect of He-Ne laser on cell survival after all being pre irradiated with UVC light and its protective effect on DNA post UV damage.
Method: This study was conducted in pathology department post graduate laboratory - College of medicine-Baghdad University. The total number of samples was (147). Blood samples were collected from healthy donors came to the blood bank, the amount of blood drown varies from 5ml to 7ml in heparin tubes .The work was carried out during the period between November 2010 to August 2011. In this experiments examination of samples was carried out to test the radiation effect on cell viability by using trypan blue dye, the experiments were preformed after 1, 24 and 72 hours post UVC irradiation to test the repair development. In other experiments Gel electrophoresis were carried out on samples to study the effect of radiation on the DNA fragmentation.
Result: Results reveal a reduced DNA fragmentation appeared on gel electrophoresis experiments as the smear length is reduced significantly for both UV10 and UV20 , other results for cell viability tests revelled that He-Ne can increase survival of cells pre irradiated with UVC irradiation giving (66%, 57%, 70%( improvement in UV exposure for10 min and (59%,56%,59%) improvement for UV exposure for 20 min respectively .
Conclusion: The effect of the laser in the improvement of cell survival may be attributed to the induction of endogenous radioprotectore and probably enzymes induced by laser irradiation which may be either reduce the free radical by scavenging effect or by improved cell repair, we may conclude that He-Ne laser can protect cells from radiation damage
This paper intends to initiate a new type of generalized closed set in topological space with the theoretical application of generalized topological space. This newly defined set is a weaker form than the -closed set as well as -closed set. Some phenomenal characterizations and results of newly defined sets are inculcated in a proper manner. The characteristics of normal spaces and regular spaces are achieved in the light of the generalized pre-regular closed set.
This study aimed to measure the alterations in serum zinc (Zn) and acute phase
proteins ( C-reactive protein and Ceruloplasmin) concentrations in patients with
visceral leishmaniasis (VL). A total of 62 individuals were enrolled in this study :
52 individuals were infected with visceral leishmaniasis and 10 individuals as
healthy control. Serum zinc levels were significantly (p<0.05) decreased in patient
group(76.25 ± 4.59 μg/dl ) when compared with healthy control (103.75 ± 3.77
μg/dl ) . C-reactive protein , as a mediator of innate immunity, removed damaged
cells by activating the classical complement pathway revealed elevated levels in
patients (4.36± 0.23mg/l ) when compared with the healthy control (2
Earthquakes occur on faults and create new faults. They also occur on normal, reverse and strike-slip faults. The aim of this work is to suggest a new unified classification of Shallow depth earthquakes based on the faulting styles, and to characterize each class. The characterization criteria include the maximum magnitude, focal depth, b-constant value, return period and relations between magnitude, focal depth and dip of fault plane. Global Centroid Moment Tensor (GCMT) catalog is the source of the used data. This catalog covers the period from Jan.1976 to Dec. 2017. We selected only the shallow (depth less than 70kms) pure, normal, strike-slip and reverse earthquakes (magnitude ≥ 5) and excluded the oblique earthquakes. Th
... Show MoreIn this study, hydroxyapatite (HAP, Ca10(PO4)6(OH)2) has been prepared as bioceramic material with biological specifications useful to used for orthopedic and dental implant applications. Wet chemical processing seems to form the fine grain size and uniform characteristic nanocrystalline materials by the interstice factors controlling which affected the grain size and crystallinity in order to give good mechanical and/or constituent properties similar as natural bone. Fluorinated hydroxyapatite [4-6 wt% F, (FHA, Ca10(PO4)6(OH)2–Fx] was developed in new method for its posses to increased strength and to give higher corrosion resistance in biofluids than pure HAP moreover reduces the risk of dental caries. The phase's and functional groups
... Show MoreTiO2 thin films were deposited by Spray Pyrolysis with thickness ((350±25) nm) onto glass substrates at (350°C), and the film was annealed at temperatures (400 and 500)°C. The structural and morphological properties of the thin films (TiO2) were investigated by X-ray diffraction, Field emission scanning electron microscopy and atomic force microscope. The gas sensor fabricated by evaporating aluminum electrodes using the annealed TiO2 thin films as an active material. The sensitivity of the sensors was determined by change the electrical resistance towards NO2 at different working temperatures (200
Abstract:
Background: The alteration of bowel habits, bleeding per-rectum and anemia were common features in both groups in this study, but in young patients there was a delay of 6 months between the presenting symptoms and the definitive diagnosis because the disease was not suspected and investigated in them. The most common site for the tumors in young patients was the rectum and in patients above the age of 40 years was the Sigmoid.
The pathological finding showed that classification of the colorectal tumors in young patients appear moderately to poorly differentiated adenocarcinoma , this indicate a more malignant course of the disease in young patients.
This study sen
... Show MoreOrthogonal polynomials and their moments serve as pivotal elements across various fields. Discrete Krawtchouk polynomials (DKraPs) are considered a versatile family of orthogonal polynomials and are widely used in different fields such as probability theory, signal processing, digital communications, and image processing. Various recurrence algorithms have been proposed so far to address the challenge of numerical instability for large values of orders and signal sizes. The computation of DKraP coefficients was typically computed using sequential algorithms, which are computationally extensive for large order values and polynomial sizes. To this end, this paper introduces a computationally efficient solution that utilizes the parall
... Show MoreRecently, important efforts have been made in an attempt to search for the cheapest and ecofriendly alternatives adsorbents. In the present work, waste molasses from Iraqi date palm (Zahdi) had been used as a provenance to produce charcoal for the removal of methylene blue (MB) dye from water. The optimum prepared charcoal was obtained at 150 C, by increasing temperature to 175 C, the charcoal had almost converted to ash. The obtained charcoal have been inspected for properties using scanning electron microscope (SEM), atomic force microscope (AFM), porosity and surface area. Adsorption data were optimized to Langmuir and Freundlich and adsorption parameters have been evaluated. The thermodynamic parameters like a change
... Show MoreLaboratory experiments were carried out in Technical college AL- Mussiab / Babylon during 2005 to study bioactivity of different concentration from ( aqueous , alcohol and hexane) extracts of bee venom collected from different workers of bees against some bacterial types that cause European Foul – brood Melissococcus plutom , Bacillus alvei and B. letrosporus which had been isolated from infected arched with disease . Two diffusion methods (digging and paper discs) were followed for the extraction of the venom . Results showed that digging diffusion method was more efficient for test of bacterial inhibition which led to increase the activity of bee venom extract with general a
... Show More