Background:Pediatric acute myeloid leukemia (AML) has a poor prognosis, and novel therapies are needed. The FLT3 tyrosine kinase inhibitorsrepresents a promising target in pediatric AML.
Objectives:This study was done to estimate the frequency of FLT3- ITD mutation in childhood acute myeloid leukemia using conventional PCR & correlate this mutation with the clinical presentation and response to induction therapy.
Patients, Materials &Methods: Twenty children with AML, and 16 children with reactive bone marrow as negative control were enrolled in this study. Those patients were attending Child Welfare Teaching Hospital in Baghdad from March 2010 to July 2011 .For each patient hematological investigations including complete blood picture, and bone marrow aspiration were done.FLT3-ITD mutation was detected by conventional PCR technology using specific primers. Complete hematological remission achievement after induction chemotherapy was assessed by clinical examinations and full laboratory investigations.
Results: Out of 20 AML children who participated in this study, 2 (10%) had FLT3-ITD mutation. The mean age of patients who had the mutation was higher than those without the mutation; and the mutated patientswere males, (P> 0.05).The FLT3-ITD mutation showed no correlation to clinical presentation.The peripheral blood & bone marrow blast cell percent werenon significantly higherin mutated patients as compared to non mutated patients. Regardingits relation to FAB classification, the FLT3-ITD mutation was only detected in M3(1/20) and M3v(1/20), and no mutation was found in other subtypes(M1,M2,M5). Furthermore, mutated patients showed lower response to inductiontherapy as compared to non mutated patients.
Conclusions: This is a noval study in one Iraqi teaching hospital to detect FLT3-ITD mutation by using conventional PCR in children with AML. This mutation was detected in 10% of thosechildren , and since they were male,older age group,and presented with higher peripheral blood &bone marrow blast cell percent thus we may propose that it may be used as a marker for the aggressiveness of the disease and can be used to modulate the treatment strategy for those patients.
In this work, monitoring of monthly variation (from May 2016 to October 2016) in the concentration of the metals (Co, Zn, Cd, Pb, Ni and Fe) from Al-Diwaniya city of Iraq. Investigation about the pollution with these metals was achieved from five selected sites locate in study area by flame atomic absorption spectroscopy. The results showed a wide variation in the levels of heavy metals from site to site and from month to month. A total of 180 surface soil samples were analyzed to detecting the pollution with selected samples. The resultsshowed that the highest concentration with Ni was 6.290 mg kg-1 while the lowest concentration detected with Ni was 0.080 mg kg-1. The results of pollution index (enrichment factor, contamination factor, po
... Show MoreThe study dosage ethanol in the total content of acid sialic TC and acid sialic associated fat (LBSA) in blood serum and congener brain Dkor Jerd eggs study included dosage 20 animals of male rat Ald ethanol daily for a period of four weeks and concentrations 20% and 30%, 40%, 50% and size of dose 5mlThe results of the study showed that levels of TSA homogeneous in the brain and blood serum significantly reduced Ankhvaza
Safe drinking water is essential for the present and future generations' health. This study aims to assess drinking water quality in Baghdad's Al-Rusafa neighborhood. Water samples were taken from 32 neighborhoods on this side. The quality of the examined potable water samples differed depending on the water source. This investigation's pH, chlorine, EC, TDS, TSS, Cd, and Pb levels were below acceptable ranges. TDS levels in Al-Mada'in are more significant than acceptable (>600ppm) water levels. Bacteria have polluted six communities (Shigella, Salmonella, Escherichia coli, and Klebsiella). Bacterial quality of drinking water and gram-negative bacteria resistant to chlorine in Baghdad's municipal water supply. Regarding pH, the w
... Show MoreThis work was evaluated the antioxidant, antibacterial and cytotoxic activity of Costus speciosus rhizomes methanol extract. The FLC analysis showed the presence of five compounds in the methanol extract of C. speciosus rhizomes. These compounds were Quercetin (5.2mg/ml), Rutin (6.02mg/ml), Luteolin, (18.3mg/ml),
Kaempherol, (11.34mg/ml) and Coumarin (1.41mg/ml). The maximum antioxidant activity of the extract was at concentration 1000 μg/ml with free radical scavenging activity approximately 67.5%. It was less than standard ascorbic acid 85.5% and Gallic acid 90% with significant difference (p≤0.05), with no significant difference
in comparison with standard TBA 68.5%. The IC50 of extract was 3093
The problem of solid waste from domestic, industrial, commercial and medical sources is one of the most important problems facing the local administration in all Iraqi cities. The danger of this problem increases with the rapid increase in the population, changing lifestyles, consumption patterns, limited land suitable for landfill, and high costs of collection and disposal. This research aims to solve these problems by determining the locations of current landfills located in the outskirts of Baghdad Governorate. The ArcGIS program was used, where the sites of the landfills were determined on the map and through the available data about the areas. it was concluded that the existing landfill sites do not meet environmental conditions and
... Show MoreIn this study found treatment mice exposed to CCl4 with the alcoholic extract of Pleurotus ostreatus (P2) at (25) mg/ml appeared to exert a beneficial effect since the activities of catalase, was significantly (p<0.05) higher in liver treatment with alcoholic extract of P. ostreatus (P2) (37.290+2.638) μmol/mg than in aqueous extract for the same fungus (36.557+2.231) μmol/mg. Super oxide dismutase (SOD) was significantly (p<0.05) higher in liver treatment with alcoholic extract of P. ostreatus (P2) at (25) mg/ml (17.863+0.454)U/mg than in aqueous extract (17.863+0.454) U/mg for the same fungus and same concentration. Glutathione peroxidase (Gpx) was significantly (p<0.05) higher in liver treatment with alcoholic extract of P.
... Show More: Clobetasol propionate (CP) is a potent corticosteroid used for skin conditions but often causes side effects due its systemic absorption. To improve its solubility and reduce it side effects (like skin irritation, skin atrophy, hypopigmentation and steroidal acne), Microsponge (Msg) has been employed as a unique three-dimensional particle that can encapsulate hydrophilic and lipophilic drugs. This study aims to develop and evaluate CP Msg-loaded hydrogels. Two Clobetasol-loaded ethylcellulose-based Msg formulas were prepared using the quasi-emulsion solvent diffusion method, then they were incorporated into Carbopol hydrogel. Two ratios of Carbopol 940 (1% and 1.5% w/w) were used. The prepared hydrogel were assessed for appearance, pH, dr
... Show MoreThe present study included the effect of chronic exposure for two concentrations of NaC? salt on the biology of fresh water zooplankton species Brachionus calyciflorus (from ^otifera). The concentrations 0.5 , 0.75%o were used for chronic exposure to investigate its effects on the life tables, range of the rate of expectation for further life and reproduction. The rotifer B. calyciflorus was sensitive to salinity and may tried to protect the species by increasing the size (2.22 ind./clutch lor control group and 2.9? for 0.5%o concentration) and number of clutches produced ?.1? clutch/female for control group and 2.9 ' ' ' for 0.75 %0 concentration) beside stimulation the animal to produce the first clutch of eggs earlier? ? small reduction
... Show MoreBackground: Trigeminal neuralgia (TN), or Tic Douloureux, is one of the most common neuropathic pains. Gamma knife stereotactic radiosurgery (GNSRS) has been considered one of the procedures for treating primary TN. Objective: This study evaluates the effectiveness of GNSRS in treating primary TN in patients who are unresponsive to medical treatment in a single-center experience. Subjects and Methods: This study was conducted on 180 patients from January 2018 to October 2021. The study utilized the Barrow Neurological Institute (BNI) pain intensity score to assess pain before and after GNSRS treatment. Results: A total of 180 patients with primary TN were included in this study, 108 female with a 1.5:1 female: male ratio. Results
... Show MoreCiprofloxacin is a broad spectrum fluoroquinolone, effective in the treatment of a wide range of infections, including genitourinary tract infections.In this study, bioadhesive vaginal tablets of ciprofloxacin hydrochloride were prepared by direct compression method using a combination of bioadhesivepolymers carbopol 934P(Cp), carboxymethylcellulose (CMC) and sodium alginate (SA) in different ratios.The prepared tablet formulations were characterized by measuring their swelling capacity, surface pH, bioadhesive properties, and in-vitro drug dissolution. It was found that the bioadhesive force was directly proportional to carbopol 934P content in different formulae and was further enhanced by the inclusion of carboxymethylcellulose.
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