Background: Toll-like receptor -2 (TLR-2)play important roles in tumor biology; by activation and promotion of tumor cell proliferation, resistance to apoptosis andalso, enhancement of tumor cell invasion and metastasis by regulating metalloproteinase and integrin’s.As toll-like receptors are widely expressed on tumor cells and participatein the initiation and progression of cancer, they may thus serve an important target and have an effective perspective on breast cancer treatment.
Objectives:The aims of the present study was to determine the levels of TLR-2 in the sera of healthy people and patients with benign and malignant breast tumors and also to investigate the validity of using TLR-2 as specific diagnostic markers of breast cancer. It`sdetection at early stage of disease could identify those patients with a high risk of progression to aggressive cancers.
Patients and methods:Thirty breast cancer femaleswith age range from 27-76 years were included in this study and they were among patients who attending the National Center For Early Detection Of Cancer - medical city complex / Ministry of Health,during the period from October 2011 to February 2012.Thirty clinically diagnosed patients with benign fibro adenoma and twenty apparently healthy women were chosen as a case control and healthy control groups respectively. For all these study groups, serum level of TLR-2 using sandwich ELISA technique was carried out.
Results:There was a statically significant difference in the serum level of TLR-2in blood of breast cancer patients and case control group in comparison to healthy controls (p >0.001),however there was no significant differences in such a level between the first two groups (p=0.44); therefore we were dealing with breast tumor cases in general regarding serum TLR-2.By using Receiver operating characteristic curve (ROC) area, in order to study the validity value of serum Toll like receptor-2 in differentiated breast tumor patients from healthy controls, serum TLR-2has the highest area under the curve (0.930) with cut off value associated with highest (perfect) sensitivity (100%) was equal to or above 0.14ng/ml.
Conclusion:The current study showed that serum levels of TLR-2were significantly higher in patients with benign and malignant breast tumors which may confirm a possible role of this marker in the pathogenesis of the disease, furthermore the best sensitivity and highest accuracy obtained from serum toll-like receptor-2 was by using a cut off values equal to or above 0.14ng/ml; Therefore, TLR-2 may be promising new diagnostic tools especially at early stages and among patients at high risk.
The most frequently diagnosed condition in women at the age of reproduction is the polycystic ovarian syndrome (PCOS).it could be related to a complex endocrine condition, due to its heterogeneity and uncertainty about its etiology, as the clinical highlights of PCOS incorporate those related to reproductive signs such as decreased frequency of ovulation, irregular menstrual cycles, decreased fertility. Carnitine plays a substantial role in weight loss, glucose tolerance, insulin function and fatty acid metabolism. Thus carnitine plays a crucial role in controlling obesity, insulin resistance, oxidative stress that are associated with PCOS .While, AGEs are a diverse group of reactive molecules that are formed end
... Show MoreBackground: Leukemias and lymphomas are malignant disorders that occur in the blood forming organs and lymphoid tissue respectively. They are classified to types and several subtypes such as acute or chronic, lymphocytic or myelocytic and T-cell or B-cell lymphocytic for leukemias and histologically into Hodgkin’s and Non-Hodgkin’s for lymphomas. Literatures do not contain many research work on magnesium in patients with these disorders, although this mineral is essential for many metabolic, enzymic, regulatory and immune reactions in the human body. Therefore, the present study was aimed to evaluate the level of magnesium in the sera of patients with different types of leukemia and lymphoma.
Patients
This study was performd on 50 urine specimens of patients with type 2 diabetes, in addition, 50 normal specimens were investigated as control group. The activity rate of maltase in patients (6.40±2.17) I.U/ml and activity rate of maltase in normal (0.44±0.20)I.U/ml. The results of the study reveal that maltase activity of type 2 diabetes patient's urine shows significant increase (P<0.01) compare to normal.
Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an eff
... Show MoreTwelve N-(6-sustirured benzothanol-2-y1) succinamic acids and 3-(6-substitted benzonathol-2-y1)-carbamoyl propionyl chloride were synthesized in good yields from reaction of benzonathol2-yl)
Understanding Caspase-3 (CASP-3) and interleukin-32 (IL32) roles in SARS-CoV-2 infection is critical to linearize the pathogenesis of the virus as well as the resultant disease which may uncover novel therapeutic targets in treating COVID-19 patients. This study aimed to evaluate caspase‐3 (CASP3) and interleukin 32 (IL32) roles and their correlation with the disease severity among patients. The case-control study (140 patients and 60 healthy controls) was performed with molecular and ELISA assays. CASP3 and IL32 serum levels were determined along with other clinical data of patients. CASP3 levels were classified as significantly higher (p < 0.001), while IL-32 levels were significantly lower in production (p
... Show MoreA simple, rapid and sensitive spectrophotometric method has been proposed for the determination of La (III) using 3-hydroxy -4-(2-hydroxy-phenyl azo) naphthalene -1- sulfonic acid as a chromogenic reagent. This method is based on the formation of a red-pink colored complex, upon the reaction of La(III) with the reagent in an alkaline medium (pH= 9.50), having a maximum absorbance at 459 nm. Beer's law is valid in the concentration range 0.512 µg.ml-1 with a Sandell's sensitivity value of 0.0188 µg.cm-2 and molar absorptivity of 7376.12 L.mol-1.cm-1. The stoichiometric composition of the chelate is 1:3. The effect of the presence of different cations as interferants in the determination of La(III) under the given optimum conditions
... Show MoreIn this research, cyclic compounds derived from 2- furfural mercaptan (oxazole, triazoles) were synthesized, and their biological efficacy was measured and compared with standard drugs. Also, their effectiveness as anti-oxidant was measured and compared with ascorbic acid as a standard substance. Some of the synthesized compounds were deduced with good efficacy. © 2021 Sami Publishing Company. All rights reserved
Objective(s): To assess the eating habits of adolescent females with iron deficient anemia.
Methodology: This study employed a quantitative research, descriptive evaluation design.The study was carried out on adolescent girls attending Kirkuk secondary school, period from 11 April to 27 December 2022. A non- probability (Purposive) sample has been applied to obtain the study goals. The study sample was (62) student who participate in the study.
Results: Pre-test results from the study revealed that 54.8% of students had moderate IDA. While the students' iron levels returned to normal in the posttest (53.2%). The majority of students (59.7%) had poor eatin
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