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`In Situ Hybridization for Detection of Latent Epstein-Barr Virus Early Repeats(EBERS) and Mutant-P53- Tumor Suppressor Gene in Patients with Non- Hodgkins Lymphoma
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Back ground: Epstein- Barr virus (EBV) is a ubiquitous in that infecting more than 90% of adult population worldwide. Recently, EBV has been linked to the development of variety of human malignancies. P53 gene is mutated in more than 50% of human cancers. Cell cycle dysregulation, measured by p53 protein expression, and latent EBV infection are important in the pathogenesis of Non-Hodgkin’s lymphomas.
Objective: To analyze the distribution and impact of concordant p53 expression and latent EBV infection on a group of B & T cell types of NHL.
Materials and Methods: Forty (40) formalin-fixed, paraffin embedded tissue blocks were obtained from lymph nodes biopsies related to patients with NHL.
In addition, biopsies of twenty (20) lymph nodes autopsies were included as apparently normal control group. The clinico- pathological criteria of NHL cases were assessed and In Situ Hybridization( ISH ) techniques for mutant p53 and Epstein-Barr Early Repeats(EBERs) detection were performed.
Results: The percentage of EBV-ISH reaction results in the total group of NHL was (60%), where its percentage in NHL, B-cell type was (58.6%)and in NHL, T-cell type (63.7%). None of the control group showed EBV-ISH reaction. Statistical analysis showed significant difference between these groups (p<0.05). The percentage of B-cell lymphoma with moderate signal score for EBV-ISH test was (41.4%) while with strong scoring was (17.2%). In the T-cell lymphoma, moderate signal scoring was (18.2%) while strong scoring was(45.5%). Statistically, they showed no significant differences (p> 0.05). Mutant p53 gene was observed in 60% of NHLs and dual positivity of EBV and p53-ISH was found in 42.5 % of cases. Regarding the grades, it was found that the higher level of p53 expression was observed in low grade tumors (39.3% ; 11out of 28) NHL.
Conclusion: The present findings indicate that cell cycle dysregulation and EBV-related transformation are important in the pathogenesis of NHL.

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Publication Date
Sat Jun 15 2019
Journal Name
Journal Of Baghdad College Of Dentistry
Perineural Invasion in Oral Squamous Cell Carcinoma in Relation to Tumor Depth
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Background: The American Joint committee on Cancer in their 8th edition staging manual regarded perineural invasion as one of the most important prognostic factors for Lip and Oral Cavity Squamous Cell Carcinoma, it also incorporated tumor depth of invasion in defining tumor size category in the new staging system. This study was conducted to evaluate the frequency of perineural invasion in oral squamous cell carcinoma and the effect of approaching tumor depth in this process. Materials and Methods: fifty-four formalin fixed paraffin embedded tissue blocks of radical resections of Oral Squamous Cell Carcinoma were cut and stained with Hematoxylin and Eosin stain, then evaluated for perineural invasion, with estimation of tumor depth of i

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Publication Date
Fri Dec 30 2022
Journal Name
Iraqi Journal Of Science
Detection of FOXP-3 Expression in a Sample of Iraqi Cervical Cancer Patients Using Immunohistochemistry Technique
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     This paper aims to find out if FOXP-3 was expressed in samples from Iraqi cervical cancer patients. Expression of FOXP-3 was detected in 55 cervical tissue samples by immunohistochemistry. Since thirty-five cases of aggressive cervical cancer were included, along with 20 normal samples used as controls. The nucleus and cytoplasm levels of FOXP-3 were counted, considering the ratio of positive cells and intensity. FOXP3 cytoplasmic staining was found in 27 out of 35 cases. Only 11 out of 35 samples displayed nuclear lymphocyte staining. Furthermore, four samples expressed this marker in both the nuclear and cytoplasm of the cervical cells. There is a highly significant difference in FOXP3 expression in the cytoplasm of

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Publication Date
Sun Dec 08 2019
Journal Name
Periodicals Of Engineering And Natural Sciences (pen)
Estimating total dissolved solids and total suspended solids in Mosul dam lake in situ and using remote sensing technique
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Publication Date
Mon Oct 01 2012
Journal Name
International Journal Of Recent Scientific Research
DETECTION OF LTH, FSH AND LH HORMONE LEVEL IN PREGNANT WOMEN INFECTED WITH TOXOPLASMA GONDII
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Infection with the protozoan parasite Toxoplasma gondii is widely prevalent in humans and animals. Infection with Toxoplasma may associate with miscarriage in many pregnant women due to infection. In this study, the level of lutetropic hormone (LTH), folliclestimulating hormone (FSH) and luteinizing hormone (LH) was measured in pregnant women suffering from toxoplasmosis using mini-VIDAS®technique. Results showed that pregnant women have high concentration of both LTH and FSH hormone(10.80 ± 6.53) ng/ml and (9.51 ± 2.40) μIU/ml respectively, while the concentration of LH hormone was lower than normal(4.49 ± 0.56) μIU/ml. Such finding is to suggest that infection with T. gondii is interfering with these hormones in pregnant women.

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Publication Date
Fri Feb 26 2021
Journal Name
Iraqi Journal Of Science
Detection of Pro-Inflammatory IL-8 and IL-12 in Iraqi Women Infected with Trichomoniasis
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Trichomonas vaginalis is a unicellular flagellated protozoan that resides in female and male genital tract and considered the most prevalent sexually transmitted infectious parasite. The infection rate is relatively equivalent between male and female but trichomoniasis is usually asymptomatic in men. Primary triggering of host inflammatory response to this parasite is not fully understood and most studies address the local reaction of the parasite in female genital tract. In this study, two pro-inflammatory cytokines, IL-8 and IL-12, were investigated in the serum of infected women with Trichomonas vaginalis during acute and chronic stages of the disease. The results demonstrated that the level of IL-8 was significantly

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Publication Date
Sun Sep 05 2010
Journal Name
Baghdad Science Journal
Detection of interleukin-6 and interleukin-8 in serum from women with recurrent spontaneous abortion
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To investigate the role of IL-6 and IL-8 in the immune-regulatory mechanisms involved in the recurrent spontaneous abortion of the first trimester of pregnancy. Serum level of IL-6 and IL-8 were determined in 25 women of age (20-35) years who had a spontaneous abortion of unknown aetiology during the first trimester of pregnancy .They were compared with the corresponding levels of 20 pregnant and non-pregnant women as control groups .cytokine levels were measured by (ELISA) technique .The women with spontaneous abortion had highly significant (P < 0.01) increased serum level of IL-8 and highly significant (P < 0.01 ) decreased level of IL-6 compared to those with normal pregnant and non-pregnant women. The results of this study ma

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Publication Date
Wed Nov 01 2023
Journal Name
Case Studies In Thermal Engineering
Augmenting the thermal response of helical coil latent-heat storage systems with a central return tube configuration
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Low-temperature stratification, high-volumetric storage capacity, and less-complicated material processing make phase-changing materials (PCMs) very suitable candidates for solar energy storage applications. However, their poor heat diffusivities and suboptimal containment designs severely limit their decent storage capabilities. In these systems, the arrangement of tubes conveying the heat transport fluid (HTF) plays a crucial role in heat communication between the PCM and HTF during phase transition. This study investigates a helical coil tube-and-shell thermal storage system integrated with a novel central return tube to enhance heat transfer effectiveness. Three-dimensional computational fluid dynamics simulations compare the proposed d

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Publication Date
Sun Mar 01 2020
Journal Name
Baghdad Science Journal
An Application of Non-additive Measures and Corresponding Integrals in Tourism Management
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Non-additive measures and corresponding integrals originally have been introduced by Choquet in 1953 (1) and independently defined by Sugeno in 1974 (2) in order to extend the classical measure by replacing the additivity property to non-additive property. An important feature of non –additive measures and fuzzy integrals is that they can represent the importance of individual information sources and interactions among them. There are many applications of non-additive measures and fuzzy integrals such as image processing, multi-criteria decision making, information fusion, classification, and pattern recognition. This paper presents a mathematical model for discussing an application of non-additive measures and corresp

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Publication Date
Mon Jan 28 2019
Journal Name
Journal Of The College Of Education For Women
Alexithymia and its relation with emotional intelleigence for 6th grade students
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This current study aims to:
1st: The recognizing of Alexithymia level for 6th grade students (Study Specimen) through the next Zero Hypothesis:1. There are no statistically significant differences at (0.05) level between the arithmetic mean of the specimen degrees as a whole and the central assumption for the scale of the lack in emotions expression
2. There are no statistically significant differences at (0.05) level between the arithmetic mean of the male students specimen and the arithmetic meanc of the female students specimen for the scale of Alexithymia.
2nd: ldentification the level of the emotional intelligence among 6th grade students (Study Specimen) through the next Zero Hypothesis:
1) There are no statistically si

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Publication Date
Thu Apr 27 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Total L-carnitine and insulin resistance in non-obese and obese Iraqi women with polycystic ovary syndrome
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Background: Polycystic ovary syndrome (PCOS) is one of the most frequent endocrine illnesses affecting reproductive - age women. L-carnitine has important roles in oxidative stress, energy production and glucose metabolism. It affects insulin resistance as decreased plasma carnitine level has been well reported in type II diabetes mellitus. Hence, it means L-carnitine may reduce insulin resistance which is found in PCO disease.

Objective: This study aims to measure the level of L-carnitine and insulin resistance in both obese and non- obese patients with PCOS.

Patients and Methods: Sixty women within the reproductive age with PCOS (30 obese and 30 non- obese) were recruited

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