Background: obesity is an important cause of adverse health problems, including male infertility. Testosterone is essential for spermatogenesis and permits the release of mature sperms. In the absence of testosterone stimulation, spermatogenesis does not proceed beyond the meiosis stage. Aromatase expression is directly related to the degree of adiposity; it is dependent on cytokine stimulation and requires the presence of glucocorticoids, 17β-estradiol in the plasma of adult men is formed by aromatization convert testosterone and androstinedione to 17β-estradiol Apoptosis is an important process in the context of germ cells since they undergo both mitosis and meiosis, and this process is affected by interleukin 6and 2 (IL6 and IL2).
Objectives: To assess the effect of body mass index on serum sex steroidal hormones, seminal antiapoptotic factor soluble fibroblast associated surface antigen (sFas) and inflammatory markers (IL6, IL-2), with conventional semen parameters in infertile men.
Patients and methods: One hundred and six male partners of infertile couples were involved in the study. Height and weight of them were measured to calculate the body mass index (BMI). Serum testosterone and estradiol 2.were measured for all.Semen sample was taken after 2-7 days of abstinence. Conventional semen analysis was done for each sample according to the protocol of (WHO) 2010, after incubation and liquefaction period (30-60 min). Semen plasma was collected for analysis of interleukins (2 and 6) and sFAS .by specific kits Patients with severe oligospermia (below million sperm/ml) were excluded from the study. The rest (77) subjects were divided into three groups according to BMI (WHO, 2010) as follows: Normal (BMI < 25 kg/ m2) (n=24): Overweight (BMI ≥ 25 and < 30 kg/m2) (n=30): Obese (BMI > 30 kg/m2) (n=23).
Results: The results showed a significant decrease in Testosterone level and Testosterone/E2 ratio in overweigh and obese subjects as compared to normal. The results of conventional semen analysis showed significant difference in pH and no. of round cell between the two groups. There was positive correlation between IL2 with sFAS in the two groups, and negative correlation of IL2 with total progressive cell and motility in both overweigh and obese groups. There was positive correlation of IL6 with no. of round cells in both studied groups.
Conclusion: BMI affects Testosterone level and Testosterone/E2 ratio (they were significantly decreased in overweigh and obese patients). A positive correlation between IL2 and sFAS in obese patients as well as positive correlation of IL6 with round cells in obese patients indicate the role of apoptosis which is essential for normal fertility to remove abnormal and unwanted cells in ejaculate, in infertile overweight and obese patients.
The interleukin-1 family has multifaceted roles in men٫s reproductive syste. Out of these is interleukin-1 receptor antagonist (IL-1RN) which exists in men gonads, and in case of infection and inflammatory process, its activity is increased. The current study aims to verify a possible linkage of Variable Number Tandem Repeat (VNTR) polymorphism of the IL-1RN gene with human men infertility. The study groups enrolled included 100 infertile men and 100 fertile and healthy men. Their seminal fluids were subjected to analysis. Also peripheral blood samples were collected for the assessment or detection of polymorphic Variable Number Tandem Repeats (VNTR) polymorphism of interleukin-1 receptor antagonist gene (IL-1RN). Two a
... Show MoreThis study aimed to explore the manufacture of high-fat pellets for obesity induction diets in male Wistar rats and determined its effect on lipid profiles and body mass index. It was an experimental laboratory method with a post-test randomized control group. Formulation of high-fat pellets (HFD) and physico-chemical characteristics of pellets were conducted in September 2019. This study used about 28 male Wistar white rats, two months old, and 150-200 g body weight. Rats were acclimatized for seven days, then divided into four groups: 7 rats were given a standard feed of Confeed PARS CP594 (P0), and three groups (P1, P2, P3) were given high-fat feed (HFD FII) 30 g/head/day. The result showed that the mean fat content of Formula II pell
... Show MoreObjective: to evaluate body image and depression symptoms of children with precocious puberty, and find out association between children`s sociodemographic characteristics and their body image and depression signs. Methodology: A cross sectional study, sample of (80) child from both gender, > 7 years were included due to their ability to express their own feeling, diagnosed with precocious puberty, attending out-patient endocrine clinics at pediatric hospitals in Baghdad city. Data collected, during the period from May to November 2018. Consent form has taken from children and their guardians to participate in study. Child body image scale (CBIS) was used to evaluate children body satisfaction (1) and Mood and feeling questionnaire (M
... Show MoreBackground: Tumor markers are often requested as part of a diagnostic workup, and increased concentrations in serum may suggest malignancy of a particular organ. However, definitive diagnosis is based on histological evaluation of the involved tissue.
Objective: The aim of present study is to evaluated CEA and CA15-3 in order to clarify at least in part their possible use as an early diagnosis tools in sera of patients with stomach, colon and rectum cancers.
Patients and methods: The study was carried out on 61 subjects comprising of 16 patients with colon cancer group (G1), 10 patients with rectum cancer group (G2), 10 patients with stomach cancer group (G3) and 25 normal healthy control The patients were selected, during the peri
The relation between anemia and inflammatory immune response has lately had much attention. This research was conducted from October 2018 until April 2019, including (110) children below 12 years from both gender in some Hospitals, Primary Health care centers, Public Primary Schools and Kindergarten in Baghdad, Iraq. The objective of this study is to determine the possible correlation between iron deficiency anemia and inflammatory immune response among children infected with Entamoeba histolytica or Giardia lamblia. Blood samples were taken from all groups to measure hemoglobin level, serum iron, total iron binding capacity (TIBC), mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration
... Show MoreDensity Functional Theory (DFT) method of the type (B3LYP) and a Gaussian basis set (6-311G) were applied for calculating the vibration frequencies and absorption intensities for normal coordinates (3N-6) at the equilibrium geometry of the Di and Tetra-rings layer (6, 0) zigzag single wall carbon nanotubes (SWCNTs) by using Gaussian-09 program. Both were found to have the same symmetry of D6d point group with C--C bond alternation in all tube rings (for axial bonds, which are the vertical C--Ca bonds in rings layer and for circumferential bonds C—Cc in the outer and mid rings bonds). Assignments of the modes of vibration IR active and inactive vibration frequ
... Show MoreBackground: Type 2 diabetes negatively affects the biochemical parameters of bone turnover more than obesity and is associated with an increased risk of osteoporosis and fragility fractures. Obesity and type 2 diabetes (T2DM) are linked to increased fracturing risk; however, the effect of obesity on diabetes-related bone deficit is unknown.
Objective: The goal of this research is to compare the indications, bone density, and bone turnover in T2DM men and a control group, and to investigate the effect of body mass index on bone turnover levels.
Subjects, Material and Method: This case-control study was conducted on 120 men whose ages were from 40 - 69 years. They were groupe
... Show MoreBackground: proteins, glycoproteins and fructose as parameters used to assess infertility in men.
Objective: To determine and correlate serum and seminal plasma of total proteins, glycoproteins and fructose with infertility in men.
Patients and Methods: The study was performed on 154 subjects; 109 infertile men (oligospermic and azoospermic) and 45 normal volunteers (normospermic men). All sera and seminal plasma were submitted for total proteins, glycoproteins and fructose levels measurment.
Results: No significant difference was noted in serum and seminal plasma of total proteins in oligospermic and azoospermic and that of normospermic men (P>0.05) compared to normospermic men. Statistical sign
Hemogloin (Hb) and serum ferritin levels are used to assess anemia in pregnancy. Some studies referred to the influence of maternal age, body mass index (BMI) and parity on Hb and serum ferritin levels. The study aimed to examine the possible association of maternal Hb and serum ferritin with maternal age, parity, and BMI in a sample of pregnant women in Baghdad.
Ninety healthy pregnant women, grouped in three equal groups according to the pregnancy trimester, and thirty apparently healthy non-pregnant women from Baghdad were enrolled in this observational study. Blood and serum samples were obtained for the estimation of Hb and serum ferritin levels.
The pooled data of participants showed a n
... Show More