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iqjmc-395
Immunocytochemical Detection of Glutathione S-Transferase in Peripheral Blood Lymphocytes of Rheumatoid Arthritis Patients
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Background: Peripheral blood lymphocytes (PBL)of Rheumatoid arthritis (RA)patients have a property of phenotypic and functional activation. Glutathione S- transferase pi (GSTπ) has been implicated in playing an important role in the initiation and progression of cellular activation.
Objectives: To determine the percentage of cellular expression of GSTπ in the lymphocytes of RA patients in comparison with controls and to explore the relation between its cellular expression and disease activity pattern.
Patients and Methods: This prospective study included46 RA patients and 17 healthy controls. Blood samples were taken and from all subjects PBL were isolated and then smeared on slides. The cellular reactivity for GSTπ was determined by immunocytochemistry technique.
Results: This study found lower expression of GSTπ in the RA patients with a statistical significant difference with control group, while no statistical difference was found in RA with high and minimum disease activity groups. No correlation was observed between GSTπ with Disease Activity Score (DAS).
Conclusion: Although the decrease in the expression of GSTπ in PBLs was pronounced in RA patients, however it doesn’t correlate with disease activity state.

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Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
Optimization of Glutathione S-Transferase in the Sera of Young Female Iraqi Patients with Diabetes Mellitus Type1
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This study was attempted to determine optimum conditions, for Glutathione s-Transferase enzyme, in sera of three groups diabetic patients type1 depending on duration of disease without complications compared with control group. The aim of this study was to find optimum conditions were determined such as (pH, Substrate Concentration, Temperature, Incubation time, Enzyme concentration, and effect of(0.15M)(0.25M) of mono divalent compounds). And to find the kinetics parameters in the three groups of diabetic patients when compared with control. It was found optimum pH(8.5,4.5,2.5,6.5).Temperatures(20cº,40cº,50cº,30cº). Incubation times (7min, 4min, 4min, 5min) substrate concentrations (12µl, 10µl, 5µl, 10µl) enzyme concentra

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Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serum Interleukine 35 in Iraqi female patients with newly diagnosed of rheumatoid arthritis.
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Background: Rheumatoid arthritis (RA) characterized by local and systemic effects of inflammation has a wide range of biochemical markers implicated directly or indirectly to its pathogenesis.

Patients and method: Serum of (55 Females) of newly diagnosed RA and 23 healthy Females were used to estimate their interlukine levels.Objective: To evaluate interlukine 35 (IL-35) in Iraqi females with newly diagnosed
RA and to assess its contribution in the disease process.

Results: Females of RA showed a significant increase in the levels of interleukine 35(IL-35) and in the levels of High Sensitivity C-Reactive Protein (hs CRP). While there

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Publication Date
Sun Oct 07 2012
Journal Name
Journal Of Educational And Psychological Researches
The effect of music therapy in lowering high blood pressure in patients with diseases of sugar
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      Faces of the individual in his life many stressful events, which includes expertise undesirable, and events may involve a lot of sources of tension and the risk factors and threats in all areas of life, and this would make the stressful events play a role in the genesis of many diseases physical.            

The high blood pressure is one of the most Actual manifestations of mental stress in the present scale physical disorders which may frequently in men relative to women, which may be caused by spasms in the blood vessels.      

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Chromosomal Aberrations in Peripheral Blood Lymphocytes in Brick Kilns Workers
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This study was conducted on the workers of the brick kilns at Al- Nahrawan area south-east of Baghdad city to investigate the effect of the pollutant emissions (fumes and gases) released from the burned fuel in this kiln. Blood samples were taken from group individuals of workers at this brick kilns and non-working individuals as a control. The influence of these emissions on the workers' health was measured by examining certain chromosomal aberrations among the workers. These aberrations were a formation of: chromosomal rings, dicentric chromosomes, breaks arm and acentric fragments. It has been observed that most of these aberrations were found within workers of age 40 years and above, represented by formations of averages 1.50 and 1.0

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Publication Date
Sun Apr 04 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Effects of Exhaustive Exercise on Peripheral Blood Lymphocytes
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Background: Acute exercise stress is associated with a lower lymphocyte functional response. It is suggested that exercise can enhance or reduce immunity depending on the frequency, duration, and intensity of the exercise.
Subjects and Methods: From November 2007 to January 2008, thirty healthy Volunteers were recruited from medical, paramedical staffs, and students of Baghdad College of Medicine (all males). Participants were chosen according to the following: (1)Age (between 20 and 35 years), (2) Non obese : Body mass index (BMI in kg/m2) was used as an index of obesity, (3) Non-smoker, (4) No recent medical problem, (5) No recent medications, and (6) No musculoskeletal complaint. After a general m

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Publication Date
Mon Jul 20 2020
Journal Name
Biochemical And Cellular Archive
DETERMINATION OF POLYMORPHISM OF GLUTATHIONE S TRANSFERASE (GST) IN THE IRAQI (DIABETIC AND NON-DIABETIC) ACROMEGALIC PATIENTS
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In Iraqi patients with acromegaly the present investigation included 40 control group and 70 patients with acromegaly divided 35 patients with Diabetic while another 35 patients without Diabetic, with ages between (29-72) years for the identification of GST activity polymorphisms by present and absent GSTM1, GSTT1, and PCR-RFLP, enzymatic digestions were carried out using BsmAI (Biolabs. England, UK) for GSTP1b and AciI (Biolabs, England, UK) for GSTP1c.the association GSTActivity with GST genotype were investigated in a cohort of Iraq acromegaly patients comparing with the healthy control group. The results show a non-significant change in GSTP1b gene in both groups, while show high significantly in GSTP1c in diabetic and non-diabetic acro

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Publication Date
Sun Oct 01 2023
Journal Name
Baghdad Science Journal
Estimation of Apelin Levels in Iraqi Patients with Type II Diabetic Peripheral Neuropathy
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Diabetes mellitus type 2 (T2DM) is a chronic and progressive condition, which affects people all around the world. The risk of complications increases with age if the disease is not managed properly. Diabetic neuropathy is caused by excessive blood glucose and lipid levels, resulting in nerve damage. Apelin is a peptide hormone that is found in different human organs, including the central nervous system and adipose tissue. The aim of this study is to estimate Apelin levels in diabetes type 2 and Diabetic peripheral Neuropathy (DPN) Iraqi patients and show the extent of peripheral nerve damage. The current study included 120 participants: 40 patients with Diabetes Mellitus, 40 patients with Diabetic peripheral Neuropathy, and 40 healthy

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Publication Date
Tue Jul 01 2008
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The role of Bcl-2 protein, Bax protein and there ratio in peripheral blood lymphocytes of asthmatic patients
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Background: Asthma is an inflammatory airway disease; this inflammatory response can be attributed to reduced lymphocyte apoptosis in peripheral blood and in airway tissues.
The mechanism behind this could be attributed to decreased Bcl2 protein and increase Bax protein in peripheral blood lymphocytes of asthmatic patients.
Aim: to explore the mechanism behind decreased lymphocyte apoptosis in peripheral blood of asthmatic patients at cellular level.
Method: Ninety four subjects; (44) control and (50) patients were included in this study during the period from (2003) to (2004). The aspirated lymphocytes for each individual
were prepared and stained by immunocytochemistry to study the percentage of antiapop

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Publication Date
Sun Mar 01 2009
Journal Name
Baghdad Science Journal
Effect Of Chlorothalonil On Some Cytogenetic Parameters Of Human Peripheral Blood Lymphocytes
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The study aimed to investigate the effect of fungicides chlorothalonil at different concentrations ( 0.1 , 0.5 , 5 , 25 , 50 ) × 10 - 5 M on some cytogenetic parameters of human peripheral blood lymphocytes . The genotoxicity parameters were estimated by the number of chromosomal aberrations (CAs) and their types and by estimating the induced micronuclei (Mn) . Cytotoxic effect recorded by estimating the mitotic index (MI) . Results revealed that the fungicide increased the CAs in dose – response pattern with positive correlation coefficient ( r = + 0.964) , there was a significant differences among the concentrations (P<0.01) . The major CAs records chromosomal breakage at concentrations. 0.5 , 5 , 25 , and 50 , while the lowest concen

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Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Oxidative Stress and Total Antioxidant Capacity in Rheumatoid Arthritis.
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Back ground:-Rheumatoid arthritis (RA) is a systemic disease characterized by progressive, erosive  and chronic poly arthritis, where cellular proliferation of the synoviocytes and neo-angiogenesis leads to formation of pannus which destroys the articular cartilage and the bone .

Objective :-to evaluate theOxidative Stress index( OSI )by measuring malondialdehide( MDA) and Total Antioxidant Capacity( TAC) in serum of RA patients in addition to uric acid and albumin and to investigate the alteration in these parameters compared to healthy individuals.

Patients and Methods :- twenty five patients with rheumatoid arithritis  and twenty five apparen

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