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iqjmc-347
Evaluation of coeliac disease serological markers
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Background: celiac disease, is an autoimmune inflammatory disease of the small intestine that is precipitated by the ingestion of gluten, a component of wheat protein, in genetically susceptible persons. Serologic tests for antibodies against Endomysium, Reticulin, and Gliadin identify most patients with the disease. Early diagnosis and management are important to forestall serious consequences of malabsorption, such as osteoporosis and anemia
Aims of the study: This study compared the sensitivity, specificity, and predictive value of, anti-reticulin and anti-gliadin antibodies according to anti endomysium antibodies , in consecutive patients investigated for celiac disease antibodies.
Patient& Methods: Total 509 patients (236 males&273 females) celiac disease patients who were referred to the immunology department in Teaching labs from January till December 2012 for serological detection of auto antibodies . Anti- Gliadin (IgG &IgA ) were measured by Enzyme Linked Immunosorbent Assay. whereas Anti-Endomyseal IgA & Anti-Reticulin IgA were detected by Indirect immunofluorescent technique on monkey oesophagus & kidney tissue respectively.
Results: Most the cases for celiac disease were reported in the age group (1-10)years as antibodies against Gliadin (IgG,IgA) ,Anti-Endomesial IgA antibodies and anti-Reticulin IgA were detected in 130(66.7%),127(65.8%),39(79.6%) and 49(68.1%) respectively. Both anti-Gliadin IgA and anti Reticulin were detected significantly in females p values( 0.047, 0.008) respectively. The study showed that anti-Gliadin IgA is the most sensitive according to anti Endomyseal IgA and anti Reticulin was the most specific for screening of celiac disease. ; While Receiver Operating Characteristic (ROC) curve showed that anti gliadin IgA had the best sensitivity and specificity according to anti Endomysium test results.
Conclusion: Predictive performance of the serological celiac disease tests showed that anti Gliadin IgA had the best sensitivity &specificity according to traditional anti Endomysium antibody test results.

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Publication Date
Thu Oct 01 2009
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Bacteriology in Adenoid Disease
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Background: Adenoidal hypertrophy is generally considered a common condition of childhood .When obstructive symptoms or recurrent otitis media (suppurative or non suppurative) occurs, adenoidectomy is generally indicated.A wide range of bacteria could be isolated from the surface and core tissue of the removed adenoid tissue.
Patients and Methods: A prospective study carried out in the ENT Department of AL-DIWANIYAH Teaching Hospital between October 2007 and September 2008.Involved 73 patients between 3 and 7 years, they were 31 male and 42 female .All patients underwent adenoidectomy .Surface and core swabs was taken from all specimens and submitted to bacteriological study.
Results: Pathogens were de

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Publication Date
Sun Oct 02 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serological Patterns of Hepstitis B Virus Among HBsAg Negative Blood Donors in Baghdad, Iraq by Using Architect System
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Background: About 10-15% of population HBV infection becomes chronic, and 85-90% are resolved. In Iraq, HBs-Ag assay by ELISA is only mandatory test for detection of HBV infection in blood donors. Anti- HBc and anti-HBs were not detectable in their sera.
Objective: To investigate the distribution of serological patterns for HBV who were HBsAg negative.
Material and method: Between July 18, 2011 to December 25, 2011, 10ml of blood samples were collected from National blood Transfusion Center donors, Baghdad, for 25782 individuals (25294 male, 488 female), mean age (20-65)years. HbsAg positive sera excluded (185), and final number of donors included in the
study was 25597 HbsAg negative.
Result: The data sho

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Publication Date
Wed Nov 24 2021
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Comparison of familial and sporadic multiple sclerosis in Iraqi patients
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Background: Multiple sclerosis (MS) is one of the increasing prevalent neurologic disorders. Epidemiologic and family studies implicate genetic and environmental factors in determining
susceptibility to MS. The exact effect of the former is intended for investigation in our study.
Objectives: The objective of the study is to compare the demographic features, clinical presenting features, and clinical course between familial and sporadic cases of MS.
Materials and Methods: this is a retrospective cohort study conducted in Multiple Sclerosis Center in the Medical City in Baghdad. The records of the MS center in Baghdad Teaching Hospital were surveyed, and data from 13 patients with positive family history of MS

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Publication Date
Sun Apr 01 2012
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Comparison of familial and sporadic multiple sclerosis in Iraqi patients
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Background: Multiple sclerosis (MS) is one of the increasing prevalent neurologic disorders. Epidemiologic and family studies implicate genetic and environmental factors in determining
susceptibility to MS. The exact effect of the former is intended for investigation in our study.
Objectives: The objective of the study is to compare the demographic features, clinical presenting features, and clinical course between familial and sporadic cases of MS.
Materials and Methods: this is a retrospective cohort study conducted in Multiple Sclerosis Center in the Medical City in Baghdad. The records of the MS center in Baghdad Teaching Hospital were surveyed, and data from 13 patients with positive family history of MS

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Publication Date
Fri Sep 30 2022
Journal Name
Iraqi Journal Of Science
Matrix Metalloproteinase-3 and Tissue inhibitor of metalloproteinase-2 as Diagnostic Markers for COVID-19 Infection
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      Coronavirus 2 is the cause of coronavirus disease 2019 (COVID-19), which leads to severe acute respiratory illness. Matrix metalloproteinases (MMPs) have been linked to leukocyte infiltration and chemokine activation during inflammatory responses. Tissue inhibitors of metalloproteinase (TIMP) family are thought to dampen the proinflammatory effects of these MMPs. The molecular pathways of lung fibrosis are mediated by MMPs and TIMPs. In this study, we sought to investigate the probable link between MMPs, specifically MMP-3, TIMP-2, and COVID-19. The study included 58 COVID-19 patients and 30 apparently healthy individuals matched in terms of age and sex. Multiplex real- time PCR was used to detect the ORF1ab, E, and N genes of

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Publication Date
Sat Jul 01 2023
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Effect of Covid-19 vaccine on some immunological salivary biomarkers (sIgA and Interleukine-17)
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Background: The most widely used vaccination against SARS-associated coronavirus (SARS-CoV-2) is the Pfizer vaccine, which provides protection against this virus. However, its ability to safeguard the oral cavity is unclear, and neither are the exact immunological biomarker levels it activates.

Aim of the study: To investigate the possibility that Pfizer vaccination protects the oral cavity against Covid-19.

Patients and Methods: The study group consisted of a total of 70 subjects (30 as the control group They were followed up before being vaccinated as non-vaccinated (maybe previously infected or non-infected or recovered) and 40 participants followed up three weeks after

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Publication Date
Wed Jun 01 2022
Journal Name
Baghdad Science Journal
Correlation between Serum and Tissue Markers in Breast Cancer Iraqi Patients
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Breast cancer is the most prevalent malignancy among women worldwide, in Iraq it ranks the first among the population and the leading cause of cancer related female mortality. This study is designed to investigate the correlations between serum and tissue markers in order to clarify their role in progression or regression breast cancer. Tumor Markers are groups of substances, mainly proteins, produced from cancer cell or from other cells in the body in response to tumor.  The study was carried out from April 2018 to April 2019 with total number of 60 breast cancer women. The blood samples were collected from breast cancer women in postoperative and pretherapeutic who attended teaching oncology hospital of the medical city in Baghdad and

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Publication Date
Sun Apr 02 2006
Journal Name
Journal Of The Faculty Of Medicine Baghdad
CEA, CA19-9 & A-FP as tumor markers for colorectal carcinoma
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Background:

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Publication Date
Fri Nov 24 2023
Journal Name
Iraqi Journal Of Science
Changes in Immune Markers for Prostate Cancer Patients Pre and Postoperation
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This study includes 15 male healthy and 30 male patients diagnosed with prostate
cancer inciude (18 cases in stages I & II and 12 cases in III – stage). All the patients
were suffered from urinary tract infection ( UTI) . The mean age is (57.33± 5.02)
years range ( 45-63) years, as cases and controls Patients with prostate cancer were
treated admits an Educational Baghdad Hospital, and Central Public Health
Laboratory and Radiation and Nuclear Medicine Hospital in Baghdad, during
period 1/6/2008 to 1/12/2010 are included in this study. The markers Prostatespecific
antigen (CA PSA), Interleukins ( IL -1 , IL-2, IL-3, IL-5) and
Immunoglobulins ( IgG, IgM, IgE) are estimated by using ELISA method. The
Cl

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Publication Date
Sun Jan 03 2010
Journal Name
Journal Of The Faculty Of Medicine Baghdad
CD-Markers as an immunological aspect in patients with visceral leishmaniasis
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Back ground: Visceral leishmaniasis is an endemic protozoan disease in Iraq. Recovery from this disease confers a solid and permanent immunity. Immunological assessment of our patients was carried out and the results showed a significant reduction in the percentage of CD3, CD56 and a significant increase in the percentage of CD19 in the peripheral blood lymphocyte of VL in comparison with control group.
Patients and methods: Indirect immunofluorescence technique analysis was performed to detect the percentage of CD3, CD19and CD56 positive lymphocytes.
Results: Our results in the patients groups showed decrease in the percentage of CD3, CD56 and increase in the percentage of CD19. Follow up of patients after

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