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Detection of Human Papilloma Virus type 6 and type 11 in women with Breast Cancer by in situ hybridization technique.
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Background: Breast cancer is one of the common malignancies among women worldwide. Human papillomavirus (HPV) infections have been linked to many human cancers in addition to cervical cancer and one of them is breast cancer.
Objective: To investigate the presence of human papilloma virus type 6 and type 11in breast cancer tissue specimens by in situ hybridization technique.
Patients and Methods: Thirty four formalin-fixed, paraffin embedded tissue blocks from breast cancer patients were obtained from the archives of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2011 to July 2012. In addition formalin-fixed, paraffin embedded blocks tissue for twenty fibroadenoma of breast were collected and used as control group. In situ hybridization technique was used for the molecular detection of HPV type 6 and HPV type 11.
Results: There were 82.4 % of breast cancer tissues positive for HPV type 6 and 88.2 % were positive for HPV type 11.
Conclusion: The presence of human papillomavirus DNA in most breast carcinoma samples merits further investigation and confirmation by second assay in order to establish the exact role of this virus in the pathogenesis of breast cancer.

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Publication Date
Thu Jan 01 2015
Journal Name
Journal Of Biotechnology Research Center
Evaluation of anti Epstien-Barr Virus antibodies in female patients with autoimmune hepatitis type-1
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Epstein-Barr Virus (EBV) infection is associated with broad spectrum of clinical manifestationsdepending on the immune status of the host, To analyze their possible role in the complication ofautoimmune hepatitis, we investigated (30) female patients with autoimmune hepatitis type-1 of(10-40)years and 25 healthy female of same ages(control groups). Both groups were carried outto measure the levels of EBV-CA IgM, IgG Ab, EBV-EA IgM, IgG Ab, and EBV-NA IgM, IgGAb using indirect immunoflourescent assay (IFAT).The prevalence of EBV-CA IgM, IgG Ab were(10%,20%) and EBV-EA IgM, IgG Ab were (10% and20%) respectively, while the prevalence ofEBV-NA IgG Ab was( 3.33%) and there are no prevalence of EBV-NA IgM Ab. There weresignificant differences (P

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Publication Date
Mon Feb 28 2022
Journal Name
Journal Of Educational And Psychological Researches
Social Support and Its Relationship to Psychological Rigidity in a Sample of Breast Cancer Patients In the Ramallah and Al-Bireh Governorate
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This study aims to identify the impact of social support on breast cancer patients’ psychological rigidity using a sample in Ramallah and al-Bireh. A descriptive correlative approach was adopted to fulfill the goals of the study and a questionnaire consisted of two criteria: social support and psychological rigidity, which was adopted as a tool for data collection for the study. In order to achieve the goals of the study, the researcher selected a convenient sample that consisted of 123 female breast cancer patients in Ramallah and al-Bireh. This sample represented 50% of the original patient population. The study showed that the average estimated percentage of social support and psychological rigidity for women with breast cancer, in

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Publication Date
Mon Dec 20 2021
Journal Name
Baghdad Science Journal
Serum prolactin, Preptin, CCL 18 and genetic polymorphisms in Iraqi women with polycystic ovary syndrome
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The polycystic ovary syndrome is an endocrine condition. One of the leading causes of female infertility and the most common disorder among women. The work was being carried out on 100 Iraqi women (50 cases confirmed with PCOS and 50 controls). Between October 2019 and March 2020, blood samples were collected from the Advanced Institute of Infertility Diagnosis and Assisted Reproductive Technology at AL-Nahrain University and a private laboratory. ELISA was used to evaluate the biochemical parameters of preptin, FSH, insulin, LH, and CCL 18 in serum samples from the AFIAS-6 (AFIAS Automated Immunoassay System). The findings of the analysis indicate that, as opposed to the control group, values of prolactin (ng/ml), LH (mIU/ml), Preptin (

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Publication Date
Mon Jan 01 2024
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Correlation of Serum levels of Chromium, Copper, and Manganese with the Glucose levels in Type 2 Diabetes Mellitus in Iraq
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Correlation of Serum levels of Chromium, Copper, and Manganese with the Glucose levels in Type 2 Diabetes Mellitus in Ira

Background: The metabolism of many trace elements such as chromium, copper, and manganese were reported to be altered in type 2 diabetes militates. This element may have specific roles in the pathogenesis and progress of this disease.

Objectives: To evaluate the serum levels of copper, chromium, and manganese in type 2 diabetic patients in Iraq. 

Patients and Methods: This case-control study included 100 type 2 diabetic patients, 35-60 years old, with high BMI as cases, and 100 non-diabetic subjects as controls who were healthy

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Publication Date
Tue Apr 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Bacterial profile of urinary tract infections in Diabetic postmenopausal women
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Background: Urinary tract infection (UTI) is the most common bacterial infection in women in general and in postmenopausal women in particular. Bacteriuria occurs more often in elderly functionally impaired women but in general many of it are asymptomatic.However the risk factors associated with recurrent UTI in elderly women are not widely described.Bacteuria in elderly women is associated with high mortality rates ,however many of thus bacteriuria are asymptomatic (Assel et al.,2009).The belief that diabetes ,a common metabolic disorder is associated with ahigher risk of UTI is widespread,diabetes result in several abnormalities of the host defense system that might result in a higher risk of certain infection.
Patient and Method: T

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Publication Date
Thu Oct 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serum Osteocalcin and Serum Osteopontin Levels in Osteoporotic Postmenopausal Women with and without Vertebral Fractures
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Background: Osteoporosis is a progressive systemic skeletal disorder characterized by low bone mass and micro-architectural deterioration of bone tissue spatially in postmenopausal women and its major complication fractures.
Objective: The aim of this study was to find out the significance of serum OC and serum OPN levels with the incidence of osteoporosis and its major complication (fractures).
Patients and Methods: Eighty-five postmenopausal women (PMW) whose ages were fifty years and over categorized into three groups: osteoporosis PMW without VFs (n=30), osteoporosis PMW with VFs (n=28), and healthy PMW (n=27). Sera samples were analyzed for alkaline phosphatase, calcium and phosphorous by using spectrophotometric kit. Serum OC

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Publication Date
Thu Jan 13 2022
Journal Name
Iraqi Journal Of Science
Association between MethylenetetrahydrofolateReductase (MTHFR) GenePolymorphisms and breast cancer in sample of Iraqi women
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Folate metabolism is fundamental and essential for DNA structure synthesis and repair. Change in genes that participate in folate metabolism can be linked with different types of malignant tumor, Therefore, this study was conducted to find out the association between methylenetetrahydrofolatereductaseMTHFR gene polymorphisms and risk of breast cancer in a sample of Iraqi patients. One Single Nucleotide Polymorphism ( SNP) including MTHFR C677T was calculated using a tetra primer ARMS PCR experiment assay. The results explained that (MTHFR C677T) consists of three genotype (CC, CT, TT), The CC genotype was the most frequent in patients and control group ( 40.00%) and(60.00%) ,respectively, while the lowest frequency was for TT genotype(26

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Publication Date
Mon Apr 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serum level of adiponectin and leptin in type 2 diabetic Cigarette smokers.
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Background: Cigarette smoking is the leading avoidable cause of morbidity and mortality in the general population; however its effects on people with diabetes are even more profound. The adipocytokines  leptin and  adiponectin  play an important role in regulating glycemia, lipidemia, endothelial dysfunction, and proinflammatory mechanisms .

Objective: We aimed to investigate the profile of  adiponectin and leptin in type 2 diabetic smokers and non- smokers.

Patient and Methods: Ninety overweight males attending the Teaching Laboratories, Medical City were enrolled in this study, they were arranged as 30 diabetics non smoker, 30 diabetics smokers and 3

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Publication Date
Thu Jan 01 2015
Journal Name
Journal Of Pharmaceutical, Chemical And Biological Sciences
The Relationship between Hepatitis C Virus Infection and Diabetes Mellitus Type 2
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To determine the relationship between hepatitis C virus infection and Diabetic mellitus type 2 , twenty patient's with diabetic mellitus type 2 aged (30-61) years old have been investigated from 01/11/2014 to 01/02/2015 and compared with fifteen parentally healthy individuals. All the studies groups were carried out to measure anti-HCV Abs by enzyme linked immunosorbent assay (ELISA), There was significant elevation (P≤0.05) in the HCV Abs compared with control groups .The percentage of HCV Abs was 15% and there was highly significant (P≤0.01) differences between studied group, while there was non-significant differences (P≥0.05) between patients groups according to age and gender compared with control groups. These results indicated

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Publication Date
Mon Dec 20 2021
Journal Name
Baghdad Science Journal
Anemia and The Level of IL-6 in Children Infected with Entamoeba histolytica and Giardia lamblia
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The relation between anemia and inflammatory immune response has lately had much attention. This research was conducted from October 2018 until April 2019, including  (110) children below 12 years from both gender in some Hospitals, Primary Health care centers, Public Primary Schools and Kindergarten in Baghdad, Iraq. The objective of this study is to determine the possible correlation between iron deficiency anemia and inflammatory immune response  among children infected with Entamoeba  histolytica or Giardia  lamblia. Blood samples were taken from all groups to measure  hemoglobin level, serum iron, total iron binding capacity (TIBC), mean corpuscular volume (MCV), and mean corpuscular  hemoglobin concentration

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