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Immunohitochemical expression of p53 in human colocrectal carcinoma
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Background:- Colorectal carcinoma is the most common cancer after the breast cancer in female and bronchus cancer in male. P53 is a tumor suppressor gene, approximately half of colorectal cancers present mutation in p53 gene.
Objectives:- To determine the frequency and the pattern of p53expression in colorectal carcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.
Materials and methods:-Thirty cases of colorectal carcinoma were included in this study, these cases were diagnosed in private pathology laboratories in Baghdad / Iraq from January 2015 to Jaune 2015. Clinicopathological parameters such as age , gender , pathological diagnosis , including the tumor site , lymph nodes status , grade and stage of tumor were taken from patients files.Sections of 4μm stained by hematoxylen and eosin stain and immunohistochemical stained for p53.
Results: Nineteen (63.3%) of the cases were males, 11(36.7%) cases were females, with age distribution ranging from (39-89) years with a mean age of 56.5 years . Ten cases(33.3%) located in the cecum , 3(10%)cases from each right colon , sigmoid 8 (26.7%) cases were from left colon and 6(20%) cases were from the rectum. Histologically the tumor grade range from moderately differentiated in 27 (90%) cases, and poorly differentiated in 3 (10%) cases. Regarding pathological staging (TNM system),2 (6.7%) cases were T2 ,24 (80%) were T3 , 4(13.3%) cases were T4. Lymph node involvement found in 14(46.7%) cases, and distant metastasis was found in 3(10%) cases. P53 expression was present in 11(36.7 %) cases, were distributed as follows:- weak in 1(3.3%) case,moderat in 3(10%) and marked in 7(23.4%) cases. There was no correlation between p53 expression and the clinicopathological parameters age , sex , histopathological grade, location , lymph nodes status and tumor stage.
Conclusion: There was no significant statistical correlation between P53 expression by tumor and different clinicopathological parameters in this study

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Publication Date
Thu Jul 28 2022
Journal Name
The Iraqi Journal Of Veterinary Medicine
Evaluating Expression of the STAG1 Gene as a Potential Breast Cancer Biomarker
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STAG proteins, which are part of the cohesin complex and encoded by the STAG genes, are known as Irr1/Scc3 in yeast and as SA/STAG/stromalin in mammals. There are more variants as there are alternate splice sites, maybe three open reading frames (ORFs) code for three main proteins, including: SA1 (STAG1), SA2 (STAG2) and SA3 (STAG3). The cohesin protein complex has various essential roles in eukaryotic cell biology. This study compared the expression of the STAG1 gene in four different breast cancer cell lines, including: MCF-7, T-47D, MDA-MB-468, and MDA-MB-231 and normal breast tissue. RNA was extracted from these cell lines and mRNA was converted to cDNA, and then expression of the STAG1 gene was quantified by three sets of specific p

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Publication Date
Wed Apr 01 2015
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Detection of Human Papilloma Virus type 6 and type 11 in women with Breast Cancer by in situ hybridization technique.
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Background: Breast cancer is one of the common malignancies among women worldwide. Human papillomavirus (HPV) infections have been linked to many human cancers in addition to cervical cancer and one of them is breast cancer.
Objective: To investigate the presence of human papilloma virus type 6 and type 11in breast cancer tissue specimens by in situ hybridization technique.
Patients and Methods: Thirty four formalin-fixed, paraffin embedded tissue blocks from breast cancer patients were obtained from the archives of the pathology laboratory of Al-Yarmouk Teaching Hospital from January 2011 to July 2012. In addition formalin-fixed, paraffin embedded blocks tissue for twenty fibroadenoma of breast were collected and used as control g

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Publication Date
Sun Jan 03 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
MicroRNAs (20a, 146a, 155, and 145) expressions in a sample of Iraqi patients with multiple sclerosis
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Background: Multiple sclerosis is a devastating central nervous system autoimmune disorder that is characterized by a series of inflammations, demyelinations, and neurodegenerations that affect the brain and spinal cord. The epigenetic studies specially micro Ribonucleic acid expression represent an important field of researches that probably uncover the obscurities behind the multiple sclerosis pathogenesis.
Objectives: to study the expression of micro Ribonucleic acids (20a, 146a, 155, and 145) in multiple sclerosis patients by the use of real time polymerase chain reaction.
Patients and Methods: A case-control study was performed using real time polymerase chain reaction technique to measure the relative expression of micro Ribo

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Publication Date
Fri Feb 08 2019
Journal Name
Journal Of The College Of Education For Women
Impact of Computer on Human Health
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As computers become part of our everyday life, more and more people are experiencing a
variety of ocular symptoms related to computer use. These include eyestrain, tired eyes, irritation,
redness, blurred vision, and double vision, collectively referred to as computer vision syndrome.
The effect of CVS to the body such as back and shoulder pain, wrist problem and neck pain.
Many risk factors are identified in this paper.
Primary prevention strategies have largely been confined to addressing environmental
exposure to ergonomic risk factors, since to date, no clear cause for this work-related neck pain
has been acknowledged. Today, millions of children use computers on a daily basis. Extensive
viewing of the compute

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Publication Date
Thu Jan 16 2020
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
Technological Literacy in Educational Programs in The Department of Accounting: As one of the Resources of Human Development
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Education is considered the corner stone of all nations development. It is the principal way for the development of human sources and the most achievements of the age due to the knowledge of these resources .                                           

   As its active role which accounting departments implement in Iraq universities , (public and private) through their teaching programs , they aim to supply labour-market with qualified cadre graduated as accountants auditors , tax auditors , financial analysts , ac

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Publication Date
Wed Jan 01 2020
Journal Name
Indian Journal Of Forensic Medicine And Toxicology
Mouse hepatocellular carcinoma sensitivity to cisplatin and docetaxel and analysis of related proteins
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Publication Date
Thu Dec 13 2018
Journal Name
Iraqi National Journal Of Nursing Specialties
The Effect of Toxoplasma Gondii on DNA SequenceAlteration among BreastCancer Patients
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Objective: The study aims to determine the effect of Toxoplasma gondii infection on the
genetic sequence of breast cancer patients in the Medical City Hospital – Tumor Unit /
Iraq-Baghdad.
Methodology: A study was carried out in the City of Medicine / Oncology Unit / Baghdad,
during the period 1st June 2016 to 15
th March 2017. Forty samples of tissue and serum
were collected from patients who complaining from Breast cancer and infected with
Toxoplasmosis. Forty sera samples were taken from patients complaining from parasitic
infection only; without breast cancer as control group. Data is analyzed by using of
descriptive and inferential data analysis methods.
Results: The results show that there is an effe

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Publication Date
Sun Jul 30 2023
Journal Name
Iraqi Journal Of Science
Analysis of Acetylcholinesterase Gene Expression in Male Mice Exposed to Sublethal Dose of Endosulfan Pesticide
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     The aim of the current study was to investigate endosulfan effects on the Acetylcholinesterase (AChE) enzyme and gene in albino mice. Thirty selected male albino mice were randomly divided into 3 groups. The first group was control group (G1), while the other two treated groups were injected intraperitoneally twice per week with two doses of endosulfanG2 (3 mg/kg) and G3 (17 mg/kg) for 21 and 45 days respectively. The results recorded a significant decrease in AChE enzyme in the group treated with 17 mg/kg b.wt. (3986.67 ±170.32 U/L),compared to the control (5584.33 ±140.35 U/L)and treatment group with 3 mg/kg b.wt.(5556.00 ±341.01) U/L for 21 days. Also, there was a significant decrease in the enzyme level

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Publication Date
Thu Jul 21 2022
Journal Name
Journal Of Advanced Biotechnology And Experimental Therapeutics,
Effect of gentamicin and doxycycline on expression of relB and relE genes in Klebsiella pneumonia
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Publication Date
Sun Jun 03 2018
Journal Name
Baghdad Science Journal
Gene Expression and Polymorphism of Interleukin-4 in a Sample of Iraqi Rheumatoid Arthritis Patients
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It was aimed to understand the interleukin-4 (IL-4) role in etio-pathogenesis of rheumatoid arthritis (RA). Two approaches were adopted. In the first one, a quantitative expression of IL4 gene was assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and such findings were correlated with some demographic, clinical and laboratory parameters, which included gender, duration of disease, disease activity score (DAS-28), rheumatoid factors (RFs), C-reactive protein (CRP) and anti-cyclic citrullinated peptide (ACCP) antibodies. In the second approach, a single nucleotide polymorphism (SNP) of IL4 gene (rs2243250) was inspected by DNA sequencing using specific primers. Fifty-one Iraqi RA patients (22 males and 29 fem

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