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Evaluation of the protective effect of Omega-7 against Methotrexate Genotoxicity in bone marrow Cells of Mice
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Background: A substance that can affect DNA or chromosomes is defined as a genotoxin. DNA damage in a somatic cell may result in a somatic mutation (cancer). In contrast, damage to a germ cell (germline mutation) may result in a heritable changed characteristic.Omega-7 is a non-essential monounsaturated free fatty acid with anti-inflammatory, anti-obesity, and antidiabetic effects.

Objectives: Evaluation of the possible protective effects of omega seven against methotrexate genotoxicity.

Method: Two major equal groups were obtained from seventy mice, and five subgroups (each of seven) were created from these groups as follows: Group I received liquid paraffin orally for seven successive days. Group II: received liquid paraffin orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group III: received omega-7 (50mg/kg) orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group IV: received omega-7 (100mg/kg) orally for seven successive days, followed by a single intraperitoneal dose of methotrexate (20 mg/kg) on the eighth day. Group V: received omega-7 (100mg/kg) orally for seven successive days. The first major group was intraperitoneally injected with 1mg/kg colchicine, and then after two hours, all mice were killed by spinal dislocation. Bone marrow cells from the first major group were used to measure the mitotic index and chromosomal aberrations, and bone marrow cell of the second group was used to measure the appearance of the micronucleus. Statistical Package for Social Sciences (SPSS) and ANOVA test were used to compare groups.

Results: Treatment of mice with omega-7 led to a significant decline in chromosomal aberration and micronucleus aberrance with a significant elevation of the mitotic index.

Conclusion: Omega-7 has been shown to have a protective role against methotrexate genotoxicity.

Received: April, 2023

Accepted: July, 2023

Published: Jan.2024

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Publication Date
Sun Jun 07 2009
Journal Name
Baghdad Science Journal
The effect of hot water and ethanol extract of Nigella sativa in immune system of Albino Mice
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The result showed that hot water and ethanol extracts of Nigella sativa contain alkaloids ,saponins, flavonoids,tannins,glycosides,terpins and steroids. Albino mice were administered orally with 0.1 of the extract at dose of 100 mg/kg, body weight the results showed high level of white blood cell ,total and differential count of WBC,phagocytosis index, mitotic index, Arthus and delayed type hypersensitivity. The result, also showed high level of hemoglobin (Hb) and the packed cell volume (PCV) the alcoholic extract has been found more efficient than hot water extract on mice.

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Publication Date
Sat Jan 04 2025
Journal Name
Journal Of Baghdad College Of Dentistry
Computed tomography bone density in Hounsfield units at dental implant receiving sites in different regions of the jaw bone
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Background: Determination of local bone mineral density (BMD) with cortical thickness and bone height may offer a comprehensive description of the bone the surgeon will encounter when he or she actually sets the implant. Quantitative computed tomography (CT) (i.e., quantitative interpretation of values derived from Hounsfield units with a suitable calibration procedure) is the modality of choice to determine BMD. The aim of the present clinical study is to determine the local bone density in dental implant recipient sites using computerized tomography. Material and method: The sample consisted of (72) Iraqi patients whom referred to Al-Kharkh General hospital, Spiral CT scan Department for bone quality and quantity assessment after one wee

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Publication Date
Tue Aug 09 2022
Journal Name
Journal Of Research In Medical And Dental Science
Evaluation of the Antibacterial Efficacy of Electrolyzed Oxidizing Water as an Irrigant against Enterococcus faecalis (An In vitro Study)
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Evaluation of the Antibacterial Efficacy of Electrolyzed Oxidizing Water as an Irrigant against Enterococcus faecalis (An In vitro Study), Noor A Khait*, Muna Saleem Kalaf

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Publication Date
Sun Mar 12 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of Copper Nanoparticles on Seminal Vesicles and Testosterone Hormone in Male Albino Mice
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The study conducted to demonstrate the effect of copper nanoparticles (Cu-NPs) on the seminal vesicle and testosterone hormone of males' albino mice. Twenty mice were used and divided into four groups control group and three groups that orally administrated with 100 mg/kg Cu-NPs for 7, 14 and 21 days and each group have 5 animals. Then, the blood was withdrawn from the animals to measure the level of the hormone testosterone in the next day after all the dosages period and then the animal was sacrificed. Seminal vesicles isolated from each animal and measured weight then histological sections were prepared to observe the changes of seminal vesicles sections. Then the morphometric was carried out to the lining cells and their nucleus and

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Publication Date
Tue Nov 13 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Tegretol (Carbamazepine) Effect on the Morphometric Assay of Liver in Female White Mice (Mus musculus)
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Carbamazepine (CBZ) is one of many anticonvulsants used to treat trigeminal neuralgia and epilepsy. Antiepileptic drugs (AED`s) are the second most important class of medications that lead to hepatotoxicity and induced liver injury, this study was conducted to evaluate the effects of CBZ on the liver. A total of 40 female mice were taken and divided into four groups (A/treated for 14 days, B/ control, C/ treated for 30 days, D/ control), the drug was given as an oral suspension formula 100mg/5ml at dose 20 mg/kg/mouse via gastric gavage daily for 14 and 30 days. Statistical analysis revealed that there were no significant differences in the white female mice body weight (P>0.05) in the treated group for 14 days as well

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Publication Date
Sun Mar 01 2015
Journal Name
Baghdad Science Journal
The Effect of Ethanolic Extract for Cyperus rotundus Rhizomes on Liver in Male Albino Mice
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The present study designed to determine the ethanolic extract of Cyperus rotundus on Liver in Albino Male Mice . In the present study 18 Male mice were used they divided into six groups ( 1st group consedered control group , 2nd group injected by 250 mg/ml from extract , 3th group injected by 300 mg/ml from extract , 4th group injected by 350 mg/ml from extract , 5th group injected by 400 mg/ml from extract and the 6th group injected by 450 mg/ml from extract ) . the expermint lasted for two days and the doses gived by intraperitonial injection . showed from the study results that ethanolic extract for Cyperus rotundus have negative effect on Liver tissue in 250 , 300 , 350 mg/ml concentrations when comparsion with control group . the re

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Publication Date
Wed Jul 08 2015
Journal Name
Ibn Al-haitham J. For Pure & Appl. Sci.
Evaluation of the Efficacy of Arbuscular Mycorrhizal Fungi in Enhancing Resistance of Lycopersicon esculentum Roots Against Fusarium oxysporum Wilt Disease
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The objective of this investigation was to study the effects of amixture of three arbuscular mycorrhizal species : Glomus etunicatum , G. leptotichum and Rhizophagus intraradices on the induced resistance of Lycopersicon esculentum roots infected with Fusarium oxysporum f.sp.lycopersici which is causal agent of wilt in the presence of organic matter peatmose (O). The work was achieved in aplastic house ( Shed) using pot culture planted for 10 weeks. Results indicated significant increase of all mycorrhizal colonization parameters ( F% , M% , m% , a% , A% ) . Highest percentage of mycorrhization was detected in roots infected with the pathogen 4 weeks after mycorrhizal colonization . On the other hand least colonization was shown in the dual

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Publication Date
Sun Apr 09 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Evaluation of the Efficacy of Arbuscular Mycorrhizal Fungi in Enhancing Resistance of Lycopersicon esculentum Roots Against Fusarium oxysporum Wilt Disease
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   The objective of this investigation was to study the effects of amixture of three arbuscular mycorrhizal species : Glomus etunicatum , G. leptotichum and Rhizophagus intraradices on the induced resistance of Lycopersicon esculentum roots infected with Fusarium oxysporum f.sp.lycopersici which is causal agent of wilt in the presence of organic matter peatmose (O). The work was achieved in aplastic house ( Shed) using pot culture planted for 10 weeks. Results indicated significant increase of all mycorrhizal colonization parameters ( F% , M% , m% , a% , A% ) . Highest percentage of mycorrhization was detected in roots infected with the pathogen 4 weeks after mycorrhizal colonization . On the other hand least colonization was s

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Publication Date
Sun Sep 07 2014
Journal Name
Baghdad Science Journal
Effect of Aspirin drug in Liver tissue of swiss white mice Mus musculus
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This study was designed to identify the extent of the effect of drug Acetylsalicylic acid (aspirin) on histological structure of liver in Swiss albino mice Mus musculus.(16) mice were used and divided into two groups. The first groupwas orally treated with )0.5ml(of aspirinat concentration of (50 mcg / kg) and the second group was considered as control group Treated with distiled water lasted for 30 days. Treatment with Aspirin led to the occurrence of histopathological cases included liver necrosis ,cellular infilitration, congestion in central vein and a case of Hemorrhage. The results also showed the absence of radial arrangement of the constituents of hepatic cells from tissue due to these changes with the control group.

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Publication Date
Sat Jun 27 2020
Journal Name
Iraqi Journal Of Science
Evaluation of the Activity of Essential Oil and Hydrosol from Eucalyptus Camaldulensis Against Some Bacterial Species
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Nowadays, the use of medicinal plants is being practiced at a wide range as a result of antibiotics resistant for the vast majority of microorganisms. Eucalyptus camaldulensis essential oil and hydrosol were used in this study against planktonic forms and biofilms of some species of Gram negative and Gram positive bacteria. The antibacterial and antibiofilm activities of this plant were detected using the microtiter plate and MIC approaches. The results proposed that the oil and hydrosol preparations have antibacterial activities against planktonic cells in different concentrations depending on the type of isolate. For antibiofilm activity, the results showed that E. camaldulensis oil was highly effective against bacter

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