Peroxynitrite is one intermediate of reactive nitrogen species with bactericidal and cylotoxic effects. Fluoroquinolones. drugs used for salmonella infections, are interacted v\ith nitrogen species and their baeterieida effect is influenced by these species. This study aims to assess serum peroxynitrite level in patients with enteric (typhoid) fever and. to investigate the effect of ciprofloxacin or amoxicillin on serum peroxynitrite level as well as in aqueous buffer solution in vitro. Thirty patients with enteric fever diagnosed clinically and serologically and twenty healthy individuals served as controls were admitted in this study. None of our sample was received anli-salmonellosis agents. Our results show that serum peroxynitrite level tended to be significantly less in patients with typhoid fever in comparison with controls. In in vitro experimental model, ciprofloxacin but not amoxicillin at 6.25 ug elevate significantly serum peroxynitrite level. In aqueous solution, the ability of ciprofloxacin to produce peroxynitrite is higher than that of amoxicillin. We conclude that Ciprofloxacin . as bactericidal agent against salmonellosis, may act via producing or elevating peroxynitrite level.
Aim: The aim of this study is to determine the correlation between levels of certain seminal biochemical parameters and serum reproductive hormones, on the one hand, and sperm function tests, on the other hand, in asthenospermic patients. Patients and Methods: Sixty asthenospermic patients and twenty fertile men as a control group were included in this study. Semen samples were collected to perform seminal fluid analysis. Total protein, cholesterol, calcium, creatine kinase, and fructose were measured in the seminal plasma. Blood samples were collected for hormonal assay of serum reproductive hormones: testosterone, prolactin, luteinizing hormone, and follicle-stimulating hormone. Results: The results revealed a significant positive correla
... Show MoreTo investigate the effects of losartan and enalapril on serum uric acid in hypertensive patients with metabolic syndrome, one hundred and twenty six newly diagnosed mild hypertensive patients, having markers of metabolic syndrome included in the study. The patients were divided into two groups. Group 1 (60 patients) was given losartan (50 mg/ day) and group 2 (66 patients) enalapril (20 mg/ day) for a duration of 2 months. A control group of seventy apparently healthy individuals were included. Metabolic syndrome was diagnosed according to diagnostic criteria of metabolic syndrome related to the American National Cholesterol Education Program-Adult Treatment Panel III. Serum uric acid levels were measured bef
... Show MoreBackground: Polycystic ovarian syndrome (PCOS) is one of the most common cause of anovulation during reproductive life.Resistin can increase ovarian androgen production by directly stimulating ovarian theca cell or indirectly by augmenting pancreatic – B cell production of insulin.
Patients and Methods: Sixty patients with PCOS who were non diabetic and not taking any medicine for the last three months were involved in the study .Thirty normal fertile female serves as control group. Fasting blood samples were aspirated from all individuals from 3rd - 6th day of the menstrual cycle to measure resistin, insulin, glucose, LH, FSH, TT3, TT4, Prolactin , Total Testosterone and lipid profile, by ELISA and rou
Axial spondyloarthritis (axSpA) is a chronic rheumatic inflammatory disease affecting mainly the spine and sacroiliac joints. Since the copper-to-zinc ratio (Cu/Zn) indicates an inflammatory response, the change in ratio is expected to correlate with axSpA. This study compared levels of Cu/Zn in the serum of axSpA patients. Serum samples were obtained from 53 patients with axSpA divided according to biological treatment into cohorts A and B, and 28 healthy control as cohort C. Serum levels of Cu and Zn were determined first by a fully automated chemistry analyzer TC-Matrix Plus, then the ratio was obtained. The elevated serum Cu concentration means of cohort B (189.32 ± 13.808 µg/dL) compared to cohort A (168.85 ± 7.244 µg/dL) a
... Show MoreBackground: Osteoporosis is a frequent disease that is manifested by reduced in mineral density and raised in fracture risk. Recent studies have indicated that osteoporosis is caused by composite connections among local and systemic regulators of bone cell function.
Objective: The purpose of this study was to investigate the relationship between interleukin-2, interleukin-4, and some biochemical markers in Iraqi patients with osteoporosis.
Patients and Methods: Forty five osteoporotic patients were incorporated in this study (30 women and 15 men). Serum fasting glucose, lipid profile, alkaline phosphatase activity, calcium, magnesium, interleukin-2, and interleukin-4 were measured in osteoporotic patients and compared them with the
Background:
Objectives : This prospective study aimed to estimate interleukins 1 and 10 levels in acute hepatitis (G) in Iraqi patients with leukemiaHepatitis G virus and GB virus C (GBV-C) RNA viruses that were independently identified in 1995, and were subsequently found to be two isolates of the same virus. Blood transfusion is the main risk factor for HGV transmission Immune-mediated mechanisms are believed to play an important pathogenic role in hepatitis G virus infection. Interleukin-1 (IL-1) plays an important role in the inflammatory process. implying that IL-1 may play a role in viral clearance and suggesting that IL-1 has direct antiviral
... Show MoreDuring infection, T. gondii disseminates by the circulatory system and establishes chronic infection in several organs. Almost third of humans, immunosuppressed individuals such as HIV/AIDS patients, cancer patients, and organ transplant recipients are exposed to toxoplasmosis. Therefore, the study aimed to investigate the possibility that Toxoplasma infection could be a risk factor for COVID-19 patients and its possible correlation with C-reactive protein and ferritin. Overall 220 patients referred to the Al Furat General Hospital, Baghdad, Iraq were enrolled from 2020–2021. All serum samples were tested for T. gondii immunoglobulins (IgG and IgM) antibodies, C-reactive protein and ferritin levels. In patients with COVID-19, the results
... Show MoreBackground: Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disease mediated by autoreactive T cells against myelin-basic proteins. Cytokines are suggested to play a role in the etiopathogenesis of the disease. Among these cytokines is interleukin-2 (IL-2). Aim of the study: To investigate the association between IL2+166 G/T single nucleotide polymorphism (SNP: rs2069763) and MS in Iraqi patients. Serum level of IL-2 was also detected. Anti-rubella IgG antibody was further determined in the sera of patients. Patients and methods: Eighty MS patients (28 males and 52 females; age mean ± SD: 39.2 ± 16.1 years) and 80 healthy control matched patients for age (32.15 ± 16.13 years) and gender (28 males and 52 females) were en
... Show MoreNew membrane electrodes for determination of ciprofloxacin hydrochloride were prepared depending on ciprofloxacin hydrochloride - phosphotungstic acid (CFH-PT) as an active material and these electrodes were made with three plasticizers: Di-octylphenylphosphonate(DOPH), Di-butyl phosphate (DBP)Tri-n-butyl phosphate(TBP), in PVC matrix. One of the ciprofloxacin electrodes was gave Nernstian slope equal to 57.21 mV/ decade for DOPH membrane with concentration range from 1.5×10-5 to1.0×10-1 M, and detection limit equal to 1.5×10-6 M .Lifetime was 93 days. Non- Nernstian responses equal to 39.40 and 30.70 mV/ decade for membranes DBP, TBP, respectively. These electrodes were gave concentration range from 1.0× 10-5 to 1.0×10-2 and from 4.0
... Show MoreHypertension is one of the leading causes of the global burden of disease, which causes serious health problems. The aim of this study is to investigate the lipid profile levels in sera of Iraqi hypertensive patients by measuring Total cholesterol (TC), triglyceride (TG) and low density lipoproteins (LDL) and kidney function levels by measuring uric acid, urea and creatinine. Seventy five individuals of Iraqi adults (Males) were divided into three groups: 25 hypertensive patients with duration of disease (1-10) year (group 1), 25 hypertensive patients with duration of disease (11-30) year (group 2) and 25 normal individuals as control group (group3). The findings indicate that serum (TC, TG and LDL) levels were significantly elevated (
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