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The effect of Fenugreek oil (Iraqi fenugreek seeds' extract) on Adultuncoupled rats and mice ovaries Histological and hormonal assay.
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Background: Fenugreek herb is one of the most abundant plants in our country. The dried ripe seeds of this plant are the effective medicinal part the plant.<br />Aim of the work: The aim of this study is to determine the pharmacological effect of a new chemical substance that has been extracted from crude fenugreek seeds which has a hormonal like action and to assess the safety of this experimental material in order to recommend it in future as a stimulator for ovulation or a contraceptive pill.<br />Materials and Methods: Sixty uncoupled female rats and mice were enrolled in this study, categorized into groups as mentioned in the text. Prolactin, estradiol and progesterone serum levels where measured for all groups. Histological and statistical analytical methods were applied to identify the increase in the folliculogenis process within the ovaries of the studied animals.<br />Results: There was an increase in folliculogenesis process in all experimental groups studied when compared to the control (group II, then group III respectively in ascending way). These findings were confirmed histologically as shown in the figures presented in the text showing the mean number of various ovarian components of experimental groups69.2±8.2 in groupli and 103.9 ±14.7 counted as the mean values these componentsand compared to the mean values of the control group which is equal to35.2 t 10.3. Hormonal assay levels, showed increase in the serum levels of hormones studied (prolactin, estradiol & progesterone) in all the experimental groups with percentage in elevation of prolactin in groups II and III were<br />(57% and 44.3% respectively). While the percentage of elevation of estradiol in groups II and III were (76% and 65% respectively). And the percentage of elevation in progesterone hormone in groups II and III were (78% and 73% respectively). The significancy of this elevation was more significant in groups IIR and IIM than. As shown in Figures (12, 13, & 14).<br />Conclusion: It has been concluded that fenugreek oil has a significant effect on folliculogenesis process within the ovary and it increases sex hormones level in the blood due to its wide biochemical effective components.<br />Key words: Fenugreek herb(Trigonella-foenum graecum). Fenugreek oil Rats(Rattus norvegicus). Mice. Ovaries. Folliculogenesis. Crude Fenugreek seeds. a contraceptive pill. Hormonal Assay. mini VIDAS technique. electrone microtome. light microscope. Histological morphometry. H & E stain (Haematoxylin&Eosin stain). Statistical analysis. Photography. Prostaglandins. tocopherol (vitamin E). haematoxylin and eosin stain.<br />

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Publication Date
Tue Mar 28 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Protective Effect of Ginger Extract Against Cisplatin-Induced Hepatotoxicity and Cardiotoxicity in Rats.
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The protective effect of ginger extract against cisplatin-induced hepatotoxicity and cardiotoxicity was evaluated in 30 albino white rats(weighing 200-300 gm ) classified into 5groups (6 rats per each group). The rats were treated with 0.5g/kg/day or         1g/kg/day ginger extract orally 5 successive days before and 5 successive days after induction of toxicity with intraperitoneal (IP) injection of (10mg/kg ) cisplatin, resulted in a significant reduction in the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) , total serum  billirubin(TSB) , lactate dehydrogenase (LDH) and creatine kinase(CK) enzymes in comparison with the cisplatin treated animals; ginger extract

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Publication Date
Fri Mar 18 2022
Journal Name
International Journal Of Health Sciences
Histological and Immunohistochemical Study of the Protective Effect of Virgin Coconut Oil on Cyclophosphamide-induced Immunotoxicity of the Spleen and Peyer’s Patches
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Publication Date
Wed Oct 31 2018
Journal Name
Iraqi Journal Of Science
The effect of Enalapril drug on some histological aspects of testes tissue in the mature albino male mice
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The study aimed to evaluate whether Enalapril has any effects on the testicular tissue of the albino male mice which received or did not receive treatment with Enalapril.

Mature mice were divided into three groups: control group which fed with 0.1mg of normal saline, the second group was orally fed with 10 mg/Kg of Enalapril drug while the third group was fed with 20 mg/Kg of drug, one time a day for 30 successive days by using gavage tube (0.1ml).  The histological sections were performed and the slides were examined using the light microscope.

 The present study result of light microscopic images of mice testes cross sections which treated with 20 mg/Kg of Enalapril showed coagulated necrosis on spermatogonia

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Publication Date
Sun Mar 01 2015
Journal Name
Baghdad Science Journal
The Effect of Ethanolic Extract for Cyperus rotundus Rhizomes on Liver in Male Albino Mice
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The present study designed to determine the ethanolic extract of Cyperus rotundus on Liver in Albino Male Mice . In the present study 18 Male mice were used they divided into six groups ( 1st group consedered control group , 2nd group injected by 250 mg/ml from extract , 3th group injected by 300 mg/ml from extract , 4th group injected by 350 mg/ml from extract , 5th group injected by 400 mg/ml from extract and the 6th group injected by 450 mg/ml from extract ) . the expermint lasted for two days and the doses gived by intraperitonial injection . showed from the study results that ethanolic extract for Cyperus rotundus have negative effect on Liver tissue in 250 , 300 , 350 mg/ml concentrations when comparsion with control group . the re

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Publication Date
Sun Sep 03 2017
Journal Name
Baghdad Science Journal
A study of histological changes in the Diaphragm of male albino mice administered with aqueous extract of chamomileflowers Chamomillarecutita
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The chamomile is one of the most important medicinal plants recommended for treatment of asthma and some respiratory system diseases. This research was designed to research the effects of aqueous extract of chamomillarecutita on histological structure of Diaphragm of albino mice. The study included 40 male albino mice Musmusculus, their age ranged from (5-7) weeks.The mices were divided randomly to 5 groups and oral administered with 1 ml every day for 10 days:- First Group G1: consider as control group and treated with normal saline,Second Group G2: was treated with aqueous extract of chamomile with concentration of 3 gm /100 ml D.W, Third Group G3: was treated with aqueous extract of chamomile with concentration of 5 gm /100 ml D.W.Fourth

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Publication Date
Fri Nov 03 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences( P-issn 1683 - 3597 E-issn 2521 - 3512)
Possible Protective Anticancer effect of Ethanol Fraction of Iraqi Hibiscus Tiliaceus L. Leaves Extract on Diethylnitrosamine-induced Hepatocarcinogenesis in Male Rats
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Abstract  Liver cancer with hepatocellular carcinoma a serious clinical illness that progresses quickly and has a bad prognosis because to increased malignancy. Fibrosis is the precursor of liver cancer, which progresses to cirrhosis and carcinoma Diethylnitrosamine (DEN) is a chemical molecule that has been used as a carcinogenic agent to promote cancer in test animals because of its strong carcinogenic potential. Herbal plants have long been used as inexpensive, effective alternatives to pharmaceuticals in various liver-associated complications, since they contain many bioactive compounds useful in liver disorders. Hibiscus tiliaceus L. (Malvaceae) contain various phytochemicals in the plant extracts such as Flavonoids, phe

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Publication Date
Sun Mar 13 2011
Journal Name
Baghdad Science Journal
Effect of Manganese Chloride in Histological Testes of Male Albino Mice
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The present study was conducted to determine the effect of Manganese on Histopathological changes in testes. Manganese Chloride was given to white mice with oral containing 150_200 and 250 mg/kg for periods of 15_30 and 45 days. The present study recorded the existence of histopathological symptoms in the testes, such as degeneration and necrosis in the tubules, congestion inside blood vessels and Edema in the interstitial tissue, as well as the appearance of giant cells inside the seminiferous tubules.

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Publication Date
Sat Oct 01 2011
Journal Name
Baghdad Science Journal
Studying the Effect of Aqueous Extract from Curcuma Longa on Some Parameters of Cytogenetic, Immunity and Fertility in Female Mice
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The research work was conducted to investigate the effect of oral administration of aqueous extract of turmeric at doses of (5, 10) mg/kg body weight for two weeks daily by determining the genotoxic effect (mitotic index), evaluation of immunological effect (IgG, IgM, IgA, C3, C4) and measuring fertility hormones (follicles stimulation hormone/FSH, lutenising hormone/LH) levels with histological examinations of female albino swiss mice ovaries in comparison with control (normal saline). A clear effect in increasing mitotic activity was reveled for both doses in comparison with control. Results also showed a significant increase in the value of all immunological parameters at both doses, in comparison with control. Also, obvious raise was se

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Publication Date
Sun Mar 06 2011
Journal Name
Baghdad Science Journal
Studying the Effect of Aqueous Extract from Curcuma Longa on Some Parameters of Cytogenetic, Immunity and Fertility in Female Mice
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The research work was conducted to investigate the effect of oral administration of aqueous extract of turmeric at doses of (5, 10) mg/kg body weight for two weeks daily by determining the genotoxic effect (mitotic index), evaluation of immunological effect (IgG, IgM, IgA, C3, C4) and measuring fertility hormones (follicles stimulation hormone/FSH, lutenising hormone/LH) levels with histological examinations of female albino swiss mice ovaries in comparison with control (normal saline). A clear effect in increasing mitotic activity was reveled for both doses in comparison with control. Results also showed a significant increase in the value of all immunological parameters at both doses, in comparison with control. Also, obvious rais

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Publication Date
Sun Dec 04 2011
Journal Name
Baghdad Science Journal
Effect of Insecticide Dichlorvos on Ovaries of Mosquito Fish Gambusia affinis (Baird and Girard)
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The toxicity of insecticide dichlorvos (90%) was tested in ovaries of mosquito fish using three sublethal concentrations (0.3, 0.6, 0.8 µg/L) and studied their effects on the means of body and ovary weights, gonadosomatic index, ovum size and ova number. Results showed that there was a significant (P<0.05) decrease in body and ovary weights, ovum size and ova count. However, there was no significant (P<0.05) difference in gonadosomatic index in treated groups with the three concentrations of dichlorvos compared with the control group. This study was added evidence on the ovular toxicity of dichlorvos concerning its effect on fish production as well as reduction in the means of productive offspring and their survival.

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