Background:
Hepatitis C Virus is the main causative agent of hepatitis among blood transfused patients, in which most chronic cases result in liver carcinoma.
Materials & Methods:
Enzyme-Linked Immunosorbent Assay for HCV, FNF- and IL-12p40 estimation with Recombinant Immunoblotting Assay (RIBA) as a confirmatory test for HCV, have been
applied for HCV detection in 80 HCV patients' samples in comparison with 30 samples for apparently healthy control, while viral load has been estimated using Branched-DNA (b-
DNA) technique for 32 randomly selected positive cases for HCV. Liver function test has been applied for patients' sera in comparison with control.
Results & Conclusions:
This study reveals highly specificity & sensitivity of ELISA technique for HCV detection which results in 100% positivity by RIBA methods. Moreover, Viral load estimation shows that (71.9%) of HCV sera samples with viral load >615 IU /ml and only (28.1%) with viral load <615 IU/ml with highly significant difference between them (P = 0.013). Furthermore, there is a highly significant variations between liver function test in comparison with control group (P< 0.01), while SGPT is the only parameter which significantly affected by viral load (P= 0.01 1). Beside that, neither INF- nor IL-12p40 level has been affected by viral loads more or less 6151U /ml. It was concluded that ELISA technique is still the best accurate reliable method for viral detection and SGPT is a good marker for highly viral loaded samples.
