Background:- Cryoprecipitate (CRYO) is the cold- precipitated concentration of factor VIII , it is prepared from fresh frozen plasma (FFP) by rapid freezing within
six hours of collection and thawed slowly between 1 - 6 Cº and removed from the supernatant . The product contain most of F VIII and part of fibrinogen from the
original plasma as well as F XIII Von Willebrand (vWF) and fibronectin 

Aim of the study: -This study is conducted to provide more information about significant contents of cryoprecipitates in regard to factor VIII, fibrinogen, and von
Willebrand factor as part of the quality assurance in blood transfusion centers and to provide competent and efficient therapeutic materials to patients with bleeding
disorders.
Materials and Methods: -In this study 98 samples were taken from the Iraqi blood donors at National Blood Transfusion Centre ( NBTC ) within 9 months from
October 2005 to the end of June 2006. The samples were arranged in two groups. Group I; 56 random samples of CRYO of different blood groups (before
modification) were used , of them 28 bags were used for FVIII and 22 bags for fibrinogen measurement . Another 25 bags were used for to measure vWF before and
after processing. Group II-A :- 16 random plasma bags were pooled together and divided into 15 bags each containing 200 ml of plasma which were frozen and thawn at ( 1-6 Cº )
after 21, 22, and 23 hours. Group II-B :- Another 25 random samples of cryoprecipitates were taken from NBTC after thawing the plasma for 22 hours and using plasma volume above 150 ml (after modification). The statistical methods used were independent sample T – test and analysis of variance (ANOVA).
Results: - In group I (n=28 ) only 6 bags (21.4 % ) contain more than 70 units of FVIII which did not meet the COE criteria , while 18 bags (81.8 %) contain more
than 140 mg of fibrinogen per bag which met the COE criteria. In group II A (n=16) thawing after 22 hours was the optimal time for separation of
FVIII (P=0.027). In group II B (n=25), FVIII and fibrinogen level separated from 200ml and 170 ml of plasma respectively were significantly more than those separated from less than
200 and 170 ml respectively (P=0.015). Moreover FVIII and fibrinogen separated from group II Bsamples were significantly more than those separated from group I. (P=0.001, P=0.027
respectively).