Background: Clostridium perfringens enterotoxin (CPE), which is one of the most common cause’s foodborne illnesses and contribute to diarrhea that is associated with broadspectrum antibiotic treatment.
Objectives: This study focuses on diagnosis of Clostridium perfringens enterotoxin (CPE) from patients suffering from food poisoning and diarrhea associated with antibiotic treatment cases in stool samples and to determine the resistance of isolated against antibiotics.
Methods: Samples were taken during the period of first of June 2015 until the end of April 2016 from Baghdad hospitals. Enzyme Linked Immunosorbent Assay (ELISA) was used to detect Clostridium perfringens enterotoxin in stool samples. Api 20A kit and culture to confirm isolation and identification was used, disk diffusion was performed for antibiotic resistance.
Results: The infection cases increased among old adult age group, were (8.7%) and their age range was (64≥) years old,and children (5.3%) their age range was(15≤) years old. Overall positivity was (23%) in present studied groups and infection increased with causes of food poisoning (61.5%).
Conclusion: This study revealed that the majority percent from age ≥64year (8.7%) and this percent decreased under this age. The future advances research should explain the epidemiology of enterotoxigenic C. perfringens and also participate to the prevention of C. perfringens food poisoning outbreaks and other CPE-associated human diseases.
In view of the huge and speedy development of the information and communications technology sectores which cover all the life aspects. The traditional government started to adopt the Electronic Government Concept to deliver the information, services and product marketing to the beneficiaries through the Internet and Computer system. The E-government became inevitable necessity that should be applied in every country wishes to face the Modern technical development.
E-government is not on alternative of the traditional government but a supporter which can increase its efficiency by keeping away from the routine and Beaurucratic complexity, this will save the efforts, increase achievement and reduce costs, in addition to Provide Man
... Show MoreDermatophytes are a group of morphologically and physiologically related molds some of which cause well defined infections: dermatophytoses (tineas or ringworm). The present study aims at identification of dermatophytes species and varieties from patients in Wasit province-Iraq using molecular approach based PCR fingerprint.
The short oligonucleotide (GACA)4 is a microsatellite primer was used in this study for identification of dermatophyte isolates. The results identified different species and varieties among dermatophytes. The numbers of resulting PCR bands ranged from 1 to 4 (size range, 600bp to 1600bp) for each species. The resulting patterns were distinct for Trichophyton and Microsporum species and varieties.
Trichophyton s
Background: The present article is concerned within the scope of Forensic Medicine, specifically Forensic Genetics. The case was taken care of in the Genetic-Molecular Laboratory of the Odessa Regional Bureau of Forensic-Medical Examinations, in Ukraine, during January and February of 2014.
Objectives: The aim of our work was to identify an unknown person, using the techniques: Y-chromosome markers and mitochondrial DNA typing.
Materials and methods: The materials available for our procedure were: pieces of tissue in paraffin blocks, saved from the corpse of the unknown person; blood from a living male subject, who claimed to be the grandfather, and from two females, allegedly the sisters. From all of them we extracted nuclear DNA
The study included the investigation of fungi ringed and inventory and Aflatoxins in rice and recorded average temperatures and humidity 22.75 degree Celsius and 13.2% respectively were obtained 1356 isolation innate possible diagnosis 15 species inherent in rice imported back to 8 races represented races b Fusarium , Cladosporium, Aspergillus and Alternaria
The plants of genus Heliotropium L. (Boraginaceae) are well-known for containing the toxic metabolites called pyrrolizidine alkaloids (PAs) in addition to the other secondary metabolites. Its spread in the Mediterranean area northwards to central and southern Europe, Asia, South Russia, Caucasia, Afghanistan, Iran, Pakistan, and India, Saudi Arabia, Turkey, and over lower Iraq, Western desert. The present study includes the preparation of various extracts from aerial parts of the Iraqi plant. Fractionation, screening the active constituent, and identification by chromatographic techniques were carried out.Heliotropium europaeum
... Show MoreWater samples from a variety of sources in Kelantan, Malaysia (lakes, ponds, rivers, ditches, fish farms, and sewage) were screened for the presence of bacteriophages infecting
Background: Hormones have very important role in spermatogenesis and production capacity of testis. Disturbances in their vlevels can be very crucial in dysfunction of testis which results in men infertility. This study carried out to evaluate the hormonal disturbances in men infertility and its correlation with semen parameters and types of infertility.
Patients and Methods: Blood and semen samples were collected from 91 infertile men and 20 healthy and fertile control who attended Al-Kadymiah hospital and some private clinics in Baghdad from January to December 2009.Semen and serum samples were analysed for semen parameter and FSH, LH, Testosterone and prolactin levels.
Results: Fifty one (56%) of in
A new series of Sulfamethoxazole derivatives was prepared and examined for antifibrinolytic and antimicrobial activities. Sulfamethoxazole derivatives bear heterocyclic moieties such as 1,3,4-thiadiazine {3}, pyrazolidine-3,5-diol {4} 6-hydroxy-1,3,4-thiadiazinane-2-thione {5} and [(3-methyl-5-oxo-4,5-dihydro-1H-pyrazol-4-yl)diazenyl] {8}. Their structures were elucidated by spectral methods (FT-IR, H1-NMR). Physical properties are also determined for all compound derivatives. Recently prepared compounds were tested for their antimicrobial activity in the laboratory. Each screened compound showed good tendency to moderate antimicrobial activity.