Preferred Language
Articles
/
iqjmc-1217
A New Method for DNA Isolation from Bone Marrow & Sperms of White Mice
...Show More Authors

Background: A quick and easy method was developed for extraction of DNA of eukaryotes from different samples, which are bone marrow and sperms in white mice Mus musculus strain (Balb/c).
Patients and Methods: this method using high salt buffer, Ethylene diemine tetracetec acid (EDTA), Trypsine,Sodium Dodecyl Sulfate(SDS), and urea without using Proteinase-K digestion or ultracentrifugation.
Results: This method was successful in extracting DNA from different samples in eukaryotic and this DNA is suitable for Hind III digestion.
Conclusion: Without further clean-up, the extracted DNA can be used for restriction endonuclease digestion or for numerous applications.

View Publication Preview PDF
Quick Preview PDF
Publication Date
Sun Jun 25 2017
Journal Name
Biomedical And Pharmacology Journal
The Impact of Toxoplasma gondii on Mitochondrial DNA of Sub-fertile Men Sperms
...Show More Authors

Toxoplasmosis is the most common, widespread disease in the world which is caused by Toxoplasma gondii.The objective of the current study is to determine the effect of the Toxoplasma gondii infection on male sperm, especially on the mitochondria of sperm for men who suffer infertility and the possibility of a hereditary mutation. Sixty seminal fluid and serum samples were taken from sub- fertile patients who attended Teba center for in vitro fertilization / Babylon and similarly samples were also obtained from healthy individuals as a control group, their ages ranged from 20 to 60 years old during the period from 1st may /2016 till 25th January/2017. All samples subjected to the tests included Macroscopic and microscopic examination, molecu

... Show More
View Publication Preview PDF
Crossref (1)
Crossref
Publication Date
Wed Sep 27 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Evaluation of the Genotoxicity of the Aerial Parts of Iraqi Euphorbia cyathophora on Bone Marrow and Spleen Cells in Mice
...Show More Authors

The aim of the study was extraction of arial part of Euphorbia cyathophora constituents with methanol and evaluate its effect on mitotic index and total chromosomal aberration bone marrow cell and spleen cell in mice  200 gm of E. cyathophora fine powder was defatted then extracted by cold maceration 80% ethanol for seven days. The extract was filtered and dried in a rotary evaporator then the dried extract was suspended with water and consecutively extracted using chloroform, ethyl acetate for each. The aqueous layer was then mixed with 100ml methanol. These fractions are dried under reduced pressure to obtain the dry extract. Twenty-four Albino mice were used for the experiment. The animals were divided into four groups: Gr

... Show More
View Publication
Scopus Crossref
Publication Date
Mon Apr 17 2023
Journal Name
International Journal Of Veterinary Science And Medicine
Donor variability of ovine bone marrow derived mesenchymal stem cell - implications for cell therapy
...Show More Authors

View Publication Preview PDF
Scopus (2)
Crossref (4)
Scopus Clarivate Crossref
Publication Date
Mon Jan 02 2006
Journal Name
Journal Of The Faculty Of Medicine Baghdad
cooagen type lll in the bone marrow of normal indivituals
...Show More Authors

Background:

View Publication Preview PDF
Crossref
Publication Date
Tue Sep 29 2020
Journal Name
Iraqi Journal Of Science
A New Beta Chaotic Map with DNA Encoding for Color Image Encryption
...Show More Authors

Images hold important information, especially in military and commercial surveillance as well as in industrial inspection and communication. Therefore, the protection of the image from abuse, unauthorized access, and damage became a significant demand. This paper introduces a new Beta chaotic map for encrypting and confusing the color image with Deoxyribonucleic Acid (DNA) sequence. First, the DNA addition operation is used for diffusing each component of the plain image. Then, a new Beta chaotic map is used for shuffling the DNA color image. In addition, two chaotic maps, namely the proposed new Beta and Sine chaotic maps, are used for key generation. Finally, the DNA XOR operation is applied between the generated key and shuffled DNA i

... Show More
View Publication Preview PDF
Scopus (6)
Crossref (2)
Scopus Crossref
Publication Date
Thu Apr 28 2022
Journal Name
Iraqi Journal Of Science
A comparative study between the effect s of Arctium lappa L. leaves extract and Pentoxifylline on DNA of sperms rats treated with Gentamicin
...Show More Authors

This study were designed to make comparison between the influence alcoholic extract of Arctium lappa L. leaves and Pentoxifylline on treatment the adverse effect of gentamicin on DNA of sperms. Thirty five male rats were divided into 5 groups: the 1st group (T1) was made as negative control group, the 2nd group (T2) was given distilled water and gentamicin 5mg/kg (positive control group), the 3rd group (T3) was given a dose 600 mg/kg of Arctium lappa L and gentamicin, the 4th group (T4) was given dose 100mg/kg of pentoxifylline and gentamicin, the 5th group (T5) was given a dose 300mg/kg of Arctium lappa extract with dose 50mg/kg of pentoxifylline and gentamicin. The results of DNA damage% and agglutination % in T3, T4 and T5 appeared an

... Show More
View Publication Preview PDF
Publication Date
Sun Apr 03 2011
Journal Name
Journal Of The Faculty Of Medicine Baghdad
A comparative study of effectiveness of diagnostic tests (r K39 strip, IFAT and bone marrow stained smear) for visceral leishmaniasis.
...Show More Authors

Background: Iraq is an endemic area for visceral leishmaniasis (VK=L) (kala-azar) which is caused by the intracellular parasite leishmania donovani.

View Publication Preview PDF
Crossref (1)
Crossref
Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Bone Marrow Procedures in Children Welfare Teaching Hospital/Medical City Complex Indications and results
...Show More Authors

Background: Bone marrow aspiration (BMA) and biopsy is a procedure that is used to evaluate the cause of abnormal blood test results, to confirm a diagnosis or check the status of severe anemia of unknown cause, to evaluate abnormalities in the blood's ability to store iron and also to diagnose infection.

Objectives: To identify the main indications of bone marrow aspiration and the most common diagnoses encountered in children welfare teaching hospital.

Patients and methods: This was a prospective and retrospective descriptive study over 6- month period from 8th of February 2010 to 8th of August 2010 in children younger than 14 years. All bone marrow aspirate results wer

... Show More
View Publication Preview PDF
Crossref
Publication Date
Tue Nov 17 2020
Journal Name
Journal Of Physics: Conference Series
A trial treatment of Hydatidosis in white mice by Immunization and Chemicals
...Show More Authors

The present study was conducted as atrial to treat secondary hydatidosis in white mice of species Mus musculus. (Balb/c strain), experimentally infected with secondary hydatid cysts of sheep origin. The immunization process was carried by using CF/PS antigen which consist hydatid cyst fluid (CF) antigen, and protoscolices (PS) antigen mixed together and by using two concentrations 7.5, 15 mg/ml. Then derivatives of Benzimidazole, Mebendazole (M) and drugs Albendazole (A) were used 10 and 40 μm/gm body weight respectively. The mixture of drugs was used one week after challenged dose. Criteria taken into consideration to define the activity of the immunization and chemotherapy in this study were reduction in the numeral of cysts, the coeffic

... Show More
View Publication Preview PDF
Scopus (1)
Scopus
Publication Date
Sat Feb 25 2017
Journal Name
International Journal On Advanced Science, Engineering And Information Technology
A Novel DNA Sequence Approach for Network Intrusion Detection System Based on Cryptography Encoding Method
...Show More Authors

A novel method for Network Intrusion Detection System (NIDS) has been proposed, based on the concept of how DNA sequence detects disease as both domains have similar conceptual method of detection. Three important steps have been proposed to apply DNA sequence for NIDS: convert the network traffic data into a form of DNA sequence using Cryptography encoding method; discover patterns of Short Tandem Repeats (STR) sequence for each network traffic attack using Teiresias algorithm; and conduct classification process depends upon STR sequence based on Horspool algorithm. 10% KDD Cup 1999 data set is used for training phase. Correct KDD Cup 1999 data set is used for testing phase to evaluate the proposed method. The current experiment results sh

... Show More
View Publication
Scopus (9)
Crossref (5)
Scopus Crossref