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iqjmc-1180
Role of mitochondria transmembrane potential in lymphocyte apoptosis
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Background: Mitochondria play an important role in the regulation of physiological type of cell death (Apoptosis) this type of cell death can be stimulated by two major pathways: external (Fas-Fas ligand interaction) and internal mitochondrial pathway which require disruption of mitochondrial transmembrane potential (ΔΨm ) which leads to opening of mitochondrial channels that lead to release of cytochrome –C which would execute apoptotic process.
Patients and Methods:The study included 32 normal subjects; 2 ml of venous blood were aspirated from each of them and processed for peripheral blood lymphocytes separation (PBL)
Then lymphocyte apoptosis was studied before and after exposing (PBL) to hyperthermia by these three methods to compare which of them can detect earliest apoptotic changes: Cell count and viability, morphological changes (by acredine orange stain) and the changes in mitochondrial membrane potential by Mitolight Apoptosis Detection Kit.
Results: Our results showed that the percentage of lymphocyte apoptosis detected by mitolight was significantly higher than those detected by other two methods.
Conclusion: This study shows that mitochondrial changes can detect earlier apoptotic process than morphological features detected by DNA stains.

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Publication Date
Mon Jun 05 2023
Journal Name
Journal Of Engineering
Potential Use of Recycled Asphalt Pavement (RAP) in Hot MixAsphalt
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The objective of the present paper is to examine the effect of Recycled Asphalt Pavement (RAP) on marshall properties and indirect tensile strength of HMA through experimental investigation. A mixture with 0% RAP was used as a control mix to evaluate the properties of mixes with 5%, 10%, and 15% RAP. One type of RAP was brought from Bab Al-moadam’s road in Baghdad for this purpose. The experimental testing program included Marshall and Indirect Tensile Strength tests. The results indicated that the bulk density, flow and VFA increase with the increasing of the percentage of RAP, while increasing in RAP results decreases in VTM and VMA values. Furthermore, the stability is changed from 10.1 kN for the control mix to12, 13.6 and 11.7 kN

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Publication Date
Wed Mar 01 2017
Journal Name
Neural Computing And Applications
The potential of nonparametric model in foundation bearing capacity prediction
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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Potential Using of Mixed Microalgae in the Bioremediation of Domestic Effluents
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Microalgae widely used for bioremediation of inorganic (NH4and PO4) nutrients as single isolates. This study aims to use a mix microalgae isolated from Dijlah river for bioremediation of municipal waste water .The experiments had been start with two different concentrations of nutrients. NH4-N values were 23.4 and 31.7mg/l, phosphorus PO4-P 6.7and 10.7 mg/ l and BOD5 were 66 and 83 mg/l. both cycles of treatment showed complete removal for NH 4-N and PO4-P levels after treatment were 1.7 and 0.7 mg/l for both of treatment cycle .Biological oxygen demand reduced to lowest value were18.9 in 2nd cycle while in 1st cycle of treatment reduced to21.3mg/l.The results showed that mix microalgae have the ability to remove nutrients and organic po

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Publication Date
Fri Aug 28 2020
Journal Name
International Journal Of Molecular Sciences
Δ9-Tetrahydrocannabinol Prevents Mortality from Acute Respiratory Distress Syndrome through the Induction of Apoptosis in Immune Cells, Leading to Cytokine Storm Suppression
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Acute Respiratory Distress Syndrome (ARDS) causes up to 40% mortality in humans and is difficult to treat. ARDS is also one of the major triggers of mortality associated with coronavirus-induced disease (COVID-19). We used a mouse model of ARDS induced by Staphylococcal enterotoxin B (SEB), which triggers 100% mortality, to investigate the mechanisms through which Δ9-tetrahydrocannabinol (THC) attenuates ARDS. SEB was used to trigger ARDS in C3H mice. These mice were treated with THC and analyzed for survival, ARDS, cytokine storm, and metabolome. Additionally, cells isolated from the lungs were used to perform single-cell RNA sequencing and transcriptome analysis. A database analysis of human COVID-19 patients was also performed t

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Publication Date
Wed Mar 30 2022
Journal Name
Iraqi Journal Of Science
Cytotoxic effects of CeO2 NPs and β-Carotene and their ability to induce apoptosis in human breast normal and cancer cell lines
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     Cerium Oxide Nanoparticles (CeO2 NPs) and β-carotene are natural-source products that have recently gained an increased interest as pharmaceutical additives because of their effectiveness in living systems, but the behavior of these substances varies according to factors and conditions. The above mentioned materials were evaluated in breast normal (HBL-100) and cancer cell lines (CAL-51 and MCF-7 ) by  different techniques ; MTT assay for studying cytotoxic effects, morphological changes, sqPCR, including gene expression of caspases 8 and 9, and P53. All experiments were conducted on cell lines by the use of the materials alone as well as their combination.                              

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Publication Date
Mon Jan 01 2024
Journal Name
Tropical Journal Of Natural Product Research
Cytotoxic Potential of Neem (Azadirachta indica A. Juss) Oil
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An increasing interest is emerging in identifying natural products to overcome drug resistance in cancer patients. In this context, the present study was conducted to investigate the cytotoxic effects of neem plant (Azadirachta indica) oil in three different biological models (breast cancer cell lines, Allium cepa root tip, and mice vital organs). The cytotoxic potential of the neem oil was evaluated with two human cell lines (MCF7 and MDA-MB231) and an Allum cepa root tip bioassay. Histopathological analysis was conducted on the neem oil-treated and untreated control mice. The results revealed an anti-proliferative effect for neem oil on both estrogen receptor-positive (MCF7) and estrogen receptor-negative (MDA-MB231) breast cancer cell li

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Publication Date
Sun Dec 31 2023
Journal Name
Tropical Journal Of Natural Product Research
Cytotoxic Potential of Neem (Azadirachta indica A. Juss) Oil
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Publication Date
Sat Oct 01 2022
Journal Name
The Egyptian Rheumatologist
Diagnostic potential of interleukin-40 (IL-40) in rheumatoid arthritis patients
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Publication Date
Mon Jun 01 2015
Journal Name
Iraqi Journal Of Science
Simulation of Solar Coronal Magnetic Field Using Potential Field Model
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In this paper, 3D simulation of the global coronal magnetic field, which use observed line of sight component of the photosphere magnetic field from (MDI/SOHO) was carried out using potential field model. The obtained results, improved the theoretical models of the coronal magnetic field, which represent a suitable lower boundary conditions (Bx, By, Bz) at the base of the linear force-free and nonlinear force free models, provides a less computationally expensive method than other models. Generally, very high speed computer and special configuration is needed to solve such problem as well as the problem of viewing the streamline of the magnetic field. For high accuracy special mathematical treatment was adopted to solve the computation comp

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Publication Date
Mon Jan 29 2024
Journal Name
Proceedings Of The International Conference On Research Advances In Engineering And Technology - Itechcet 2022
Liquefaction potential effect in Makhool Earth dam under seismic impact
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This study is directed at investigating the liquefaction potential within earth dams using numerical modelling by two-dimensional finite element analyses method for considering the Makhool earth dam on the Tigris River in Iraq. The effect of peak ground acceleration of 0.02g, 0.04g, 0.06g, and 0.08g is viewed for a shell, and the crest is presented for all scaled earthquake duration 25 s, 50 s, 75 s, and 100 s. The current study program comprises selecting a representative history point within the Makhool earth dam as a case study. Many points were allocated at different locations within the shell and crest to observe the fluctuation in the factor of safety against liquefaction. The seepage analysis results viewed graphically for the operat

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