Background: Hydatid disease also known as echinococcosis or hydatidosis , is caused by infection with larva (metacestoide) of tape worms of the genus Echinococcus.
Materials and methods: Twelve Rabbits were immunized with three types of antigens (Hydatid cyst fluid antigen; Protoscoleces antigen and Germinal and Laminated layer antigen) according to a specific immunization program and we used indirect hemagglutination test( IHAT) and Enzyme Linked Immuno Sorbent Assay(ELISA) for detecting the anti-Echinococcus antigens in the serum of these animals.
Results: The highest titter of antibodies were recorded in the Rabbit serum when use the first antigen (Hydatid cyst fluid) by using IHA method (1:64)after the end of immunization program while in ELISA method reached (1:128), The serum level for the rabbit serum that immunization with the second antigen(Protoscoleces) has reached (1:256) by using IHAT and (1:512)when use ELISA, The highest titter of third antigen (Germinal and Laminated layer)has reached (1:128) by using IHAT and (1:256) when we used ELISA method.
Conclusion: The highest antibodies titer could be found when use the Protoscoleces antigen more than Cyst fluid and Germinal and Laminated layer antigen.
Fifty one patients with serologically confirmed brucellosis and 70 healthy controls were phenotyped for HLA-A, -B, -DR and -DQ antigens by using standard microlympho-cytotoxicity method, and lymphocytes defined by their CD markers (CD3, CD4, CD8 and CD19). The results revealed a significant (Pc = 0.001) increased frequency of HLA-DR8 (41.18 vs. 10.0%) in the patients . A significant increased percentage of CD8+ lymphocytes was also increased in the patients (25.15 vs. 22.0%; P = 0.006), while CD3+ lymphocytes were significantly decreased (75.1 vs. 79.4%; P = 0.02).
An enzyme linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin G (IgG) antibodies against vero- cytotoxine (VT) producing Escherichia coli serogroup O157:H7 was produced. E. coli O157: H7 lipopolysaccharide was extracted from locally isolated strains by using hot phenol- water method, followed by partial purification using gel filtration chromatography by sepharose- 4B. The purity of the lipopolysaccharide was checked by measuring the protein and nucleic acid content and then used as antigen. Four isolates of vero- cytotoxin producing E. coli serogroup O157:H7 was obtained by culturing 350 stool samples from children suffering from bloody diarrhea. These isolates were identified on bacteriological, s
... Show MoreBackground: Traumatic brain injury (TBI) is a serious public health problem. Even injuries classified as mild which is the most common, can result in persistent neurobehavioural impairment. Diffuse axonal injury is a common finding after TBI, and is presumed to contribute to outcomes, but may not always be apparent using standard neuroimaging. All severities of TBI can result in a degree of axonal damage, while irreversible myelin damage was only apparent for moderate to severe TBI.
Objectives: To evaluate the effects of moderate closed head injury on visual evoked response (VER) and to assess the changes in VER 6 months later as follow up indices.
Subjects and methods: T
... Show MoreThe thermal distribution in the diseased tissue treated by different methods faces the problem of an uncontrollable defused heat. In the present article, we use a plasmonic bowtie nanoantenna working in the near infrared region to enhance the temperature confinement in the tissue. The Computer Simulation Technology Studio Suite package version 2019 was used to execute the design of both plasmonic nanoantenna and the tissue. Gold nanostructure and silicon carbide dioxide are the components the plasmonic nanoantenna in the bowtie shape. The results showed that the distance between the tumor tissue and the antenna is important to determine the intensity field where the maximum field is 5.9*107 V/m at a distance of 100 nm. The maximum
... Show MoreHydatid cyst disease is one of the most common diseases in many places in the world. The infection occurs when human and livestock drinking or eating contaminated water and food with eggs of Echinococcus granulosus worm. Surgery is the best solution to eradicate cysts and rapid healing, but it may be accompanied by some risks such as rupture of the cyst and leakage its contents of protoscolices, which leads to the return of infection and spread in the body. Several methods have been used to reduce the risks of surgery, including withdrawal of hydatid fluid and its contents and injection scolicidal substances like ethanol and others. Researchers have recently tested the efficiency of nanoparticles such as selenium, silver, and gold nanoparti
... Show MoreDiabetes is a chronic disease which has reached pandemic proportions in a lot of countries of the world. In spite of the remarkable development in therapeutics chemistry, the usage of customary drugs is still a common practice for the treatment of diabetes. The current study was designed to investigate the effects of pomegranate seed oil (PSO) on kidney functions in the experimentally-induced diabetes in male rabbits. Diabetes was experimentally introduced by the intraperitoneal injection of alloxan monohydrate (150mg/kg BW). After three days of alloxan injection, samples were taken for the determination of glucose concentration. Serum glucose concentration of 200mg/ml was considered as an indication of animal diabetes. The experimental
... Show MoreChildren who have a health problem need special requirements for their clothes. The purpose of the study is to design functional and aesthetic clothes to meet their needs. The research used the applied descriptive approach. The research sample comprised 120 women whose children suffered from hip dislocation. Designs for injured children presented and submitted to the arbitrators for evaluation regarding the functional and aesthetic aspects. The finding was achieving the design solutions. The most important recommendations are to increase attention to designing clothes to suit all the needs of society.
The long healing time of bone after tooth extraction in order to construct artificial teeth is uncomfortable to the patient because of aesthetic or masticatory problems in addition to the daily visit to dental clinic. The objective of this study was to evaluate the effect of 805 nm diode laser with long time intervals on repair of bone and skin incisions in rabbits through biochemical, radiological and histological findings. Eighteen New-Zealand rabbits were undergone surgical operations to make a cavity in the bone of the lower jaw, the rabbits were divided into two groups:- Group A (control group) containing nine rabbits. Group B (lased group) containing nine rabbits in which two cavities were done, one on the right side and the other
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