Background: Ischemic heart diseases (I.H.D) become the most common cause of sudden death, and is also the most common reason for death of man and women over 20 years of age in the world. Many Factors Play a role in pathogenesis of I.H.D. among those could be Immune – inflammatory markers which may lead to development of this disease.The present study was conducted to obtain more clarification about the impact of some immuno – inflammatory markers (IL- 8 , IL- 4 and IFN- Ճ) on the Clinical expression of heart disease among Iraqi patients .
Patients and methods: Hundred Iraqi patient with I.H.D. (80 male and 20 female) ages ranged between 20 to 80 year were involved in this study, include 4 subgroups: 17 with heart failure (H. F) ; 26 with myocardial infraction (M.I) ; 12 with stable angina (S.A) and 45 with unstable angina (U.A) who attending the Iraqi Center for Heart Disease and Baghdad Hospital from December 2006 to march 2007 . For quantitative estimation of serum levels of IL- 8, IL- 4 and IFN- Ճ by Enzyme Amplified Sensitivity Immuno Assay (EASIA) technique for both patients and healthy control groups.
Results: Significant increased (P< 0.05) in the serum level of interleukin – 8 in all patient subgroups: H.F., M.I, S.A and U. A. respectively Compared with control group, also all patient subgroups: H. F., M. I, S.A and U. A. Consequently revealed Significant increased (P< 0.05) in the interleukin – 4 Concentration compared with control group. More over, highly Significant increased (P< 0.01) in the interferon – Ճ concentration in the patients Subgroups: H. F, M. I, S.A and U. A respectively compared with control group.
Conclusion: Elevated level of inflammatory Markers (IL- 8, IL- 4 and IFN – Ճ) in all patients of I.H.D reflects the importance immuno – inflammatory elements as risk factor in the pathogenesis of Heart Disease.
This study is to evaluate plasma levels of several cytokines in preeclamptic pregnancies compared to those of healthy pregnancies. Ninety pregnant women with preeclampsia (37 mild & 53 severe) and thirty healthy pregnant women were enrolled in the study. Blood samples were taken and plasma levels of IL-8, IL-10, and IFN-? were measured by enzyme-linked immunosorbent assay (ELISA). Preeclamptic women and their severe cases but not mild cases had significantly (P<0.05) increased levels of plasma IL-8, and IFN-? as compared with healthy pregnancies. By contrast, plasma levels of IL-10 was significantly (P<0.05) increased in healthy pregnant women as compared to all groups of preeclampsia. Preeclampsia is associated with an imbalance betwe
... Show MoreTrichomonas vaginalis is a unicellular flagellated protozoan that resides in female and male genital tract and considered the most prevalent sexually transmitted infectious parasite. The infection rate is relatively equivalent between male and female but trichomoniasis is usually asymptomatic in men. Primary triggering of host inflammatory response to this parasite is not fully understood and most studies address the local reaction of the parasite in female genital tract. In this study, two pro-inflammatory cytokines, IL-8 and IL-12, were investigated in the serum of infected women with Trichomonas vaginalis during acute and chronic stages of the disease. The results demonstrated that the level of IL-8 was significantly
... Show MoreBackground: the different roles of cytokines IL-4, IL-10, IL-12p40, IFN- during acute toxoplasmosis disease in pregnant women.
Determination of Interleukin-8 (IL-8) Levels In Pneumonia Patients
Background: Cytokines are the messengers of the immune system.They are mostly secreted by macrophages and lymphocytes and their production is induced in response to injury or infections.
Objective: Biopsy speciemens from the middle body of the stomach were obtained from 18 patients with gastric carcinoma, 32 patients with atrophic gastritis, 50 patients with chronic gastritis and 20 healthy subjects and IL-8 and IL-1B levels were detrmined.
Methods: Sandwich-type enzyme-linked immunosorbent assay kit was used to determine the IL-8 and IL-1B levels.
Results: IL-8 and IL-1B levels were significantly higher in gastric carcinoma patients than patients with atrophic gastritis and both significantly higher than healthy subjects.
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In this work an enzyme linked immunosorbent assay (ELISA) technique has been used for detection of some inflammatory markers in serum of acute coronary syndrome (ACS)-Patients Admitted to the cardiac care unit (CCU) of Iraqi Centre For Heart Diseases and Ibn AlNafees Teaching Hospital. The present method includes quantitative measurement of interleukine-6 (IL-6) and C-reactive protein (CRP), as their increase during symptoms may be responsible for identifying the mechanism of myocardial damag, in addition to their best performance than other quantitative tests perhaps due to their association with atherosclerotic process that belongs to the endothelial dysfunction. Aim of this study is to estimate the prevalence and correlation of IL-6 w
... Show MoreBackground: Tinea versicolor is a common dermatological problem ,world wide distribution , caused by a dimorphic fungus called Malassezia furfur ,it live normally on skin as a commensal . Many factors play a role in the etiology of TV among these could be the disturbed immune system which may be related to the ability of TV alter the immune system by a process called Immunomodulation leading to subsequent infection. Immuno-inflammatory activity mediated by different cytokines could have a role in the etiology of TV.
Objective: To evaluate the serum level of the inflammatory cytokines ,IL-4andIL-8,in patients with TV as compared to immunocompramized patients and healthy groups.
Patients and methods
Background: Periodontal disease increases local and systemic inflammatory responses in pregnant women, which may lead to premature delivery.
Objectives: To detect maternal serum levels of proinflammatory cytokine (Interleukine-6, Interleukin-8) with preterm delivery in pregnant women suffering from gingivitis.
Cases and methods: In this case-control study, a total of 85 pregnant women, were included in the study, of whom 55 had gingivitis: 25 preterm deliveries and 30 full-term deliveries. Thirty healthy pregnant women with healthy gingiva were in the control group. They were selected from Al-Ramadi Teaching Hospital for Gynecology and Pediatrics, from November 2022 to May
... Show MoreBackground: Visceral Leishmaniasis( VL ) is a systemic infection of the reticulo- endothelial system that could affect the immune system and biochemical parameters like the concentration of Copper which may be significantly hanged .
Objective: The study aims to evaluate the level of cytokines (INF- γ ,IL-10 ) and trace element (Cu ) in Visceral Leishmaniasis in children after diagnosis .
patients and Methods: A total of 98 children, whose their ages ranged (6 menthes -5 years) were attending the Central Public Health Laboratory and Teaching Laboratories of Medical City, who were suspected to be infected with kala- azar, who were diagnosed by both IFAT technique and Rapid Kala-azar (r-K39) detecting test and trying to evaluate the