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The Relationship between Phylogenic Typing and Antimicrobial Susceptibility Patterns forEscherichia coliIsolatedfrom UTIs atMany Hospitals in Baghdad City
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Objective:The current study aime to isolate Escherichia colifrom urinary tract infections(UTIs) in many Baghdad hospitals. The study concentrate on phylogenic groups and this was done based on triplex PCRmethod by primers besieged to three genetic markers, chuA, yjaA and TspE4.C2. Evaluate the relationship of phylogenic groups of E. coli isolates with the antibiotic-non sensitive patterns. Methodology:Four hundredof E.coli bacteria isolated from urine samples from five hospitals in Baghdad city include: Ghazi AL-Hariri, Ibin- Al-Beledi , AL-Iskan , AL-Nooman and AL-Yarmoke hospitals. Phylogenetic categorization of E. coli isolates was completed by by means ofearlier reported triplex PCR-based on phylotypingprocedureusing primers besieged at three markers, chuA, yjaAand TspE4.C2. Phylogenetic combination was done on the starting point of the existence or nonexistence of the3 DNA fragments as follows: chuA–, TspE4.C2–, group A; chuA–, yjaA–, TspE4.C2+, group B1; chuA+, yjaA+, group B2; chuA+, yjaA–, group D. Because two possible profiles can be obtained for the groups A, B2, and D. Fifteen antibiotics second-hand for all foremost groups and their individual generations were used in this paper adjacent to all bacterial isolates. Results: The isolates ofE.colifrom UTI were distributed within thephylogroups B2 (43%);phylogroups D (28.25%);phylogroups A (24%) and B1 (3.5%), so phylogenic group B1 was prevalent among male patients of AL-Yermouk hospital ( 14%), followed AL-Iskan (3.75)% so, isolation rate of E.coli were higher among age (11-20 ) years (31.75 %), also(11%) were sensitivity to antibiotics, whilst (75.25%) were classified as extensive drug resistance pathogens, but (13.75% )were multidrug resistance and in Karkh areas, the frequency of phylogeneticB2 was (59.88%), followed phylogenetic A and D were ( 68.75 and 48.78%) respectively as compared to Rusafa areas were prevalence phylogenetic B2(40.12%),followedphylogenetic D and A were (51.22 and 31.25 %) , as well as most of these phylogenetic groups occurred more frequently in both groups B2 or D (43, 28.25%) respectively, also ( 2.75, 2.5% ) of phylogenic A were antibiotics sensitivity in both AL-Iskan hospital and Ghazi AL-Hariri hospital respectively whilst these phylogenic was multidrug resistance as percentage (9, 3.75) % respectively in both AL-Yermouk hospital and AL-Iskan hospital, Whilst in these hospital this phylogenic A was founded with extensive drug resistance (1, 0.75%) respectively, also most of phylogenetic group B2 occurred more frequently in AL-Yermouk hospital, so the sensitivity; multidrug resistance and extensive drug resistance as (2.25, 15 and 3.5%) respectively, also all phylogenetic groups E. coli isolates from UTI patients of five hospital weregrouped in two or three major group as A , B group contain major sub groups . Recommendations: The study recommends additional studies of the correlation between the Phylogenic group with antibiotic-resistance pattern for other bacterial types isolated from UTIs or the same bacteria isolated from other types of infections. From this study,it is recommendedto study onKlebsillaspp isolated from the infected patients urinewho submitted to the same hospitals orin the other provinces of the country.

 
 

 

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Publication Date
Fri Dec 30 2011
Journal Name
Al-kindy College Medical Journal
Early and late Biliary Complications of Laparoscopic Cholecystectomy in Acute Cholecystitis
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Background: Laparoscopic cholecystectomy
has become the standard of care for the
elective management of cholelithiasis. Little
information exists, however, regarding the
appropriateness of this procedure in the setting
of acute symptomatology.
Objective: This study was designed to
evaluate the outcome of laparoscopic
cholecystectomy in acute and severe acute
cholecystitis based on early and late biliary
complications, their incidence and
management, and conversion rates to open
surgery.
Methods: A prospective study done between
April 2007 and November 2010, in the
department of general surgery, medical city
teaching hospital, Baghdad. Includes patients
with acute cholecystitis admitted f

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Publication Date
Mon Jan 01 2007
Journal Name
Atti Della “fondazione Giorgio Ronchi”
Photo-physical characterization of Rhodamine B in liquid and solid solutions
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SUMMARY. – Absorption, flourescence, quantum yield and lifetime of rhodamine B in chloroform, methanol and dimethyl sulfoxide were measured. A comparison was done of these quantities with those for solid solutions, which are obtained by mixing constant volume proportions of dye at a concentration of 1×10–4M/l with different volume proportions from the concentrated solution of polymer in chloroform and dimethyl sulfoxide. The results showed that the addition of polymer to liquid concentrated solutions (1×10–4M/l) of rhodamine B dye from expecting, which leads to development of active medium for laser dye at high concentration, increase the spectra shift toward high energies, and the luminescence quantum yield but decreasing radiative

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Publication Date
Fri Jan 18 2019
Journal Name
Asian Journal Of Chemistry
Determination of Chemicals and its Isotopic Composition in Tigris River,Iraq
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Stable isotope (?18O, ?D) values were determined along with the chemical compositions at 10 different locations along the Tigris river between Baghdad-Ammara cities of Iraq. The physico-chemical parameters and isotopic data were measured. The sampling site represents 34 % of total Tigris river in the republic of Iraq. The systematically increased in values of stable isotope as move from the downstream of the river and the most significantly appears at Kut lake. This increase occurs as a result of several factors, viz. (a) evaporation occurs low water level in the river and its tributaries, and (b) return flow water to the river from irrigation water in groundwater systems. The change in ion distribution and in the isotopic values related di

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Publication Date
Sun Jan 01 2017
Journal Name
Journal Of Central European Agriculture
Seed priming effect on field emergence and grain yield in sorghum
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Most cultivars of sorghum have low field emergence as common problem. Three experiments were carried out at Agriculture College, Baghdad University. First experiment was conducted in laboratory according to Complete Randomized Design (CRD) with four replicates. Second and third experiments were conducted at field according to Randomized Complete Block Design (RCBD) during the spring and the fall seasons of 2015, respectively, with four replicates for each one. Same two factors were studied at each experiment. First factor was seed priming by seed soaking for 10 hours in distilled water, solvents of GA3, KCl and thiamine (300, 40 and 30 mg*L-1, respectively) and non-primed seed. Second factor was three cultivars of sorghum (Inqath, Kafier an

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Publication Date
Thu May 18 2023
Journal Name
Journal Of Engineering
Experimental and Theoretical Investigation of Noise Effect in Centrifugal Fan Impeller
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In this work a study was made in centrifugal fan blower to investigate the effect of impeller blade design on sound pressure level (SPL). Shroud and unshroud impeller of nine blades are used. The sound generation from flow inside the test rig at different positions was displayed by using spectral analyzer. The experiments were carried out in anechoic chamber with small holes in its walls, under ambient condition about (25-27) C ° to avoid the effect of temperature on the sound pressure level. The results showed that (SPL) decreased with the increase of distance from the source about (3-4)dB when distance varied about (0.8-1.06)m, and the (SPL) decreased with the decrease of velocity about (8-12)dB when velocity varied between (13000-260

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Publication Date
Sat Jan 01 2022
Journal Name
Materials Today: Proceedings
Application of levofloxacin and doxycycline in treatment of vaginal chlamydial infection
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Doxycycline and levofloxacin are heterocyclic organic compounds that are now popularly used to treat some bacterial infections. In this study which was conducted on patients from a private clinic, we compared the use of two commonly used antibiotics for the treatment of diagnosed cases with vaginal chlamydial infection: levofloxacin and doxycycline. Vaginal chlamydial infection is considered to be a very common sexually transmitted infection. It may have minimal symptoms which can lead to neglect by the patient until it leads to some important complications among which is the inability to become pregnant. This indicates early diagnosis and treatment of this infection. By comparing the results of treatment we concluded that there is no much

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Publication Date
Sat Jul 01 2017
Journal Name
Bulletin Of The Iraq Natural History Museum (p-issn: 1017-8678 , E-issn: 2311-9799)
EFFECT OF HONEY ON SPERM CHARACTERISTICS AND PREGNANCY RATE IN MICE
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    The aim of the current study is to demonstrate the effect of honey on the sperms characteristics (sperm concentration, sperm motility, grade of activity and sperm normal morphology) as well as pregnancy rate in mice. Sperms were obtained from caudal epididymis of male mice and prepared by adding10% of honey to the IVF medium using direct sperms activation technique for 30 minute incubation period before artificial insemination.

 

    The study revealed a significant (P > 0.05) increase in active sperm motility (grade A and grade B) 49% and pregnancy rate 53.3% in female mice artificially inseminated with sperms. On the other hand, there were no significant differences in sperm con

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Publication Date
Tue Jul 02 2024
Journal Name
Al-rafidain Journal Of Medical Sciences ( Issn 2789-3219 )
Formulation and Evaluation of Bilastine Thermosensitive Mucoadhesive Ophthalmic in situ Gel
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Background: Bilastine is a non-sedating, second-generation antihistamine used to treat urticaria and allergic conjunctivitis. Objective: to formulate and test bilastine as a mucoadhesive ophthalmic in situ gel in order to extend its presence at site for longer time and help treat conjunctivitis and allergic rhinitis. Methods: We prepared formulations using different concentrations of poloxamers (Poloxamer 407 (P407) and Poloxamer 188 (P188)) in combination with hydroxypropyl methyl cellulose (HPMC). The prepared formulas were evaluated for their physicochemical properties, sol-gel transition temperature, viscosity, mucoadhesive strength, drug release, and kinetic modeling. Results: The prepared in situ gels were clear and transparen

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Publication Date
Wed Jun 01 2022
Journal Name
Annals Of The Faculty Of Arts
Motivation and Second Language Acquisition: Motivational Orientations in Foreign Language Learning
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Numerous research studies have been conducted on why some learners acquire a second language more easily and quickly than others. Most of these studies have demonstrated that acquiring a second language does not depend only on learners’ cognitive ability or professional teaching strategies. The learning language process is more complicated than that. It is affected by crucial factors that are beyond the control of learners and teachers. These factors are known as sociolinguistic factors. These factors include culture, age, motivation, socio-economic status, and gender. This research paper mainly concentrates on the role of motivation in second language acquisition.

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Publication Date
Sun Aug 01 2021
Journal Name
Bulletin Of Electrical Engineering And Informatics
Robust speaker verification by combining MFCC and entrocy in noisy conditions
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Automatic speaker recognition may achieve remarkable performance in matched training and test conditions. Conversely, results drop significantly in incompatible noisy conditions. Furthermore, feature extraction significantly affects performance. Mel-frequency cepstral coefficients MFCCs are most commonly used in this field of study. The literature has reported that the conditions for training and testing are highly correlated. Taken together, these facts support strong recommendations for using MFCC features in similar environmental conditions (train/test) for speaker recognition. However, with noise and reverberation present, MFCC performance is not reliable. To address this, we propose a new feature 'entrocy' for accurate and robu

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