The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fermentum and L. plantarum) were more competitive than food lactobacilli (L. acidophilus and L. bulgaricus).

   In this study the Individuals of Ostracoda crustacean Cyclocypria were used as bioclaner of two species of bacteria namely Escherichia coli & Staphylococcus aurous, these bacteria have been introduced to an artificial aquatic environment with known number from pure cultures. The aim was to check the predation efficiency and ecological role of Cyclocypria as biological cleaners.      The results showed that the predation rate was between 6 106 ×  and 7× 10 6 colony/ ml/ 3 days/ 10 crustacean in the first three days. This rate increased remarkably in the 9th, 12th & 15th day from the beginning of the experiment and it was 10 × 10 6 and 15 × 10 6 respectively.  &nb

            Toxoplasma gondii is an opportunistic parasite in immune-compromised persons. The prevalence of toxoplasmosis in psoriasis patients is investigated. In addition, the treatment effect on psoriasis patients infected with toxoplasmosis through evaluating Tumor Necrosis Factor-α (TNF-α) cytokine levels is studied. Blood samples were collected from 130 individuals who involved 60 control samples and 70 samples with psoriasis. They attended Medical City Hospital in Baghdad province from October 2017 - February 2018. Then, the anti- T. gondii antibodies (IgM and IgG) and TNF- α in the sera were determined via the enzyme linked immune-sorbent assay. The highe

Background: Until today, the etiology of recurrent aphthous stomatitis (RAS) remains unknown, although hints of its etiologic basis lay on genetic susceptibility, infectious agents and alterations in immune mechanics.
Objectives: The aim of this study was to investigate the possible alterations in salivary tumor necrosis factor-alpha (TNF-α) and Immunoglobulin-A (IgA) level in patients with RAS and its relation with clinical types of disease.
Subjects and Methods: Salivary TNF-α levels were investigated in 50 RAS patients and 25 healthy controls by enzyme-linked immunosorbent assay (ELISA). Single radial immune diffusion method was used for estimation of IgA level in two studied groups.
Results: S

Extensive studies have been conducted on the microbial properties of Streptococcus pneumoniae  all over the world ,but there are few studies in Iraq on the most important factors of virulence possessed by S.pneumoniae isolates found in Iraq , 195 of sputum specimens were collected from patients with pneumonia acquired from the community who were clinically diagnosed by specialized doctors depending on symptoms and  Radiography of Chest . Eighteen isolates of  S.pneumoniae were  diagnosed  by  special traditional methods that used in the phenotypic identification . All isolates 18 (100%) have been  given  positive results for  the optochin test , bile solubility test

Background:  One of the most dominant bacterial infections is urinary tract infection (UTI), both in the community and in the hospital settings Since drug resistance become one of the predominant problems of health worldwide, it is necessary to use new methods to overcome drug-resistant bacteria. In this regard, medicinal plants (Medicago sativa )  are considered one of the richest sources to produce antibiotics.

Aim of the study: evaluate the antibacterial effect of the fraction of ethyl acetate of Medicago Sativa extract against Escherichia Coli isolated from urine samples of patients with urinary tract infections.

Material and methods:

Mycobacterium tuberculosis is the cause of the major world health issue, tuberculosis (TB). The cytokine, tumor necrosis factor alpha (TNF-α) has been implicated in protection against TB in the early stages of the disease. TNF-α is an effective cytokine in the killing of intracellular M. tuberculosis. This study inducted to investigate whether there is any relationship between levels of TNF-α in sera of TB patients and their recovery, and is there any difference in the level of this cytokine in sera of female and male TB patients. This study included 29 patients with pulmonary TB (18 female and 11 male), their ages ranging from 37 to 59 years. All of them received first line TB therapy. They were consulted at Pasture Center during Septem

Twenty-seven S. aureus isolates were obtained from patients referring various hospitals in Baghdad. Only 17 isolates produced DNase. SNase was extracted and purified from Saphylococcus aureus 3 isolate since it produces the largest zone of clearance on DNase agar. Nevertheless, only those phenotypically-producer of DNase harboured nuc gene. Present study revealed that the crude enzyme had a specific activity of 50.66 unit/mg; while it reached 241 unit/mg after ion exchange chromatography using carboxymethyl cellulose column. SDS-PAGE showed a single sharp band with an approximately 16.8 kDa molecular weight. A matter indicates that the enzyme is consistently pure. Results proved that SNase was able to significantly (P< 0.05) reduce th

Reactive arthritis (ReA) has been as joint developing after infection, it belongs to spongylo arthritis (SpA). The etiology of this disease was multi factorial, the combination between genetic and environmental factors for triggering this disease. This study included 75 Iraqi Arab patients and 39 healthy control. Urine samples and blood were collected from each subject. The results showed that Escherichia coli bacteria (E. coli) was isolated from 32% of urine samples. HLA-B*27 allele frequencies was higher in ReA patients infected with E. coli. This lead to suggest that E. coli may be trigger factor in ReA patients with UTI which had HLA-B*27 positive.

Interferon’s plays a role in innate immune responses through upregulation of costimulatory molecules and induction of proinflammatory cytokines. Interferon alpha (IFN α) type of Interferons. The present study characterized IFNα cDNA . The interferon’s play a great role in protection from infections, caused by microorganisms, and have powerful antiproliferative and immunomodulation activity. In this study DNA was isolated from bovine blood leukocyte, which was used in the quality of matrix for amplification of α-interferon gene with the use of PCR, and isolation of gene α-interferon and transformation in vector pUC18 and expression vector pET24b (+). All plasmids contained an additional DNA fragment size corresponding to the gene