The isolates were screened according to their capability for pectinase production, screening process identified the best pectinolytic isolate and it was characterized by cultural and biochemical, as Pesudomonas sp. Pectinolytic enzyme producing bacterium Pesudomonas sp. was isolated from the Iraqi soil on nutrient agar plate. Optimiztion of process parameters were carried out by altering some of environmental conditions of chemo-physical environment for the production medium. The highest pectinase production was observed at 48 hrs of incubation at 35 °C with the initial pH of 6.0. Different nutrients and environmental conditions were investigated in terms of their effect on the production of extracellular pectinase using citrus pectin as substrate. The results exhibited that Yeast extract 0.15% with 0.5% of citrus pectin supported maximum pectinase production in the optimum conditions.
Biogas is one of the most important sources of renewable energy and is considered as an environment friendly energy source. The major goal of this research is to see if rice husk (Rh) waste and pomegranate peels (PP) waste are suitable for anaerobic digestion and what effect NaOH pre-treatment has on biogas generation. Rice husk and pomegranate peels were tested in anaerobic digestion under patch anaerobic conditions as separate wastes as well as blended together in equal proportions. The cumulative biogas output for the blank test (no pretreatment) was 1923 and 2526 ml, respectively using a single rice husk (Rh) and pomegranate peel (PP) substrates. The 50% rice husk digestion and 50% of pomegranate peels for blank test gave the result 224
... Show MoreThis study was done to determine the effect of watery and alcoholic extracts of Elettaria cardamomum on growth of bacteria isolated from Otitis media Infections which are : Pseudomonas aeruginosa , Staphylococcus aureus and Klebsilla spp. The biochemical analysis revealed that watery extracts of Elettaria cardamomum contains: Glycosides, Alkaloids, Saponines, Tannins, and Volatile oils, ( This compound is Soluble in water ) , while the alcoholic extracts contains : (in addition to the above mentioned compounds ) Phenols , Resins , Flavonids and Coumarins. ( This compound is Soluble in organic solutions ). To study the e
... Show MoreMost approaches to combat antibiotic resistant bacteria concentrate on discovering new antibiotics or modifying existing ones. However, one of the most promising alternatives is the use of bacteriophages. This study was focused on the isolation of bacteriophages that are specific to some of commonly human pathogens namely E. coli, Streptococcus pyogenes, Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella spp. and Klebsiella pneumoniae. These bacteriophages were isolated from sewages that were collected from four different locations in Kirkuk City. Apart from S. pyogenes, bacteriophages specific to all tested bacteria were successfully isolated and tested for their effectiveness by spot test. The most effective
... Show MoreUrinary tract infections (UTIs) mean microbial pathogens in the urethra or bladder (lower urinary tract). Important risk factors for recurrent UTI include obstruction of the urinary tract, use of a bladder catheter or a suppressed immune system. This study aims to isolate and identify bacteria from patients with TCC-bladder cancer or patients with a negative cystoscope and estimate antibiotic susceptibility patterns and evaluate some of the virulence factors. From a total of 62 patients with TCC-BC or negative cystoscope, only 35 favorable bacterial growths were obtained, including Escherichia coli (UPEC), a significant bacterial isolate, and Stenotrophomonas maltophilia. The percentage of multi drug-resistance bacteria
... Show MoreElectro-kinetic remediation technology is one of the developing technologies that offer great promise for the cleanup of soils contaminated with heavy metals. A numerical model was formulated to simulate copper (Cu) transport under an electric field using one-dimensional diffusion-advection equations describing the contaminant transport driven by chemical and electrical gradients in soil during the electro-kinetic remediation as a function of time and space. This model included complex physicochemical factors affecting the transport phenomena, such as soil pH value, aqueous phase reaction, adsorption, and precipitation. One-dimensional finitedifference computer program successfully predicted meaningful values for soil pH profiles and Cu
... Show MoreBackground: Urinary tract infections (UTIs) and their complications such as Bladder cancer (Bl. C.) are a health growing problem worldwide. Objective: To shed light on this subject, present study was done to investigate relationship between recurrent urinary tract infection (RUTI) due to Escherichia coli (E. coli) and Bl. C.Type of study: Cross-sectional study. Methods: This study included 130 patients with RUTI, 50 patients with Bl. C. and 50 control of both sexes (aged 7-85 years) attending Al-Zahra Teaching Hospital in Al-Kut/Wassit governorate and Al-Harery Teaching Hospital of specialized surgeries/Baghdad. The patients were divided into two groups: the first group (n=130) included those who were suffering from recurrent UTI without
... Show MoreThe tested isolate of Rhizobium leguminosarum bv. viciae was isolated from root nodules of vicia faba plant from Baghdad ,the isolated bacteria was examined for bioemulsifier production when growing in mineral salt medium that containing 1% sunflower oil as sole carbon source . Rhizobium isolate was able to produce bioemulsifier with initial emulsification index E24% was 38%. Many of physical and nutritional cultural conditions for optimum emulsifier production was examined including : pH , temperature ,incubation period ,carbon and nitrogen source. The maximum bioemulsifier production form Rhizobium isolate was 64.5% when bacteria growing in mineral salt medium with pH 9 ,incubated at temperature 30 ºC , incubation period was 10 days ,
... Show MoreBackground: A Catheter-associated with candidiasis infection is the most common nosocomial infection and the objective of this work is to isolate and identify Candida species from catheterized patients by ordinary culture and PCR.Objective:To study the isolation and identification of Candida species from catheterized patients by culture media and polymerase chain reaction(PCR).Methods: One hundred and thirty five Candida species isolates were obtained from urine culture of catheterized specimens from male and female patients , During the period between October 2011 to April 2012 , attending AL-Ramadi general teaching Hospital. A quantitative urine culture for isolation and identification of Candida species was. The isolation of Candida s
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