Klebseilla pneumoniae possesses many virulence factors and survival strategies to persist and overcome host defenses; one of these strategies is biofilm formation. Therefore, the aims of this study was to determine the antibacterial and antibiofilm effect of Rosmarinus officinelis L. essential oil (EO) and its effect on the genes encoding of fimbrial adhesions. The antimicrobial activity was investigated by MIC. The ability to form biofilm as well as inhibition of initial cell attachment and biofilm formation was performed. PCR was carried out to detect fimH-1 and mrkD genes of type 1 and type 3 fimbrial adhesions at different time of incubation. The study revealed that MIC value of EO was 104 μg/ml on 24 (83%) of isolates, 93% of them produced biofilm. Fifty percent reduction in biofilm formation was observed in 10% of isolates at concentration 104 μg/ml and increased to 45% when used 1.5×104 μg/ml of EO. PCR product of fimH-1 was detected at 24 h but absence at 0 and 4 h while mrkD product found in all incubation time. In conclusion, Rosemary EO had antibacterial and antibiofilm activity against Klebsiella pneumoniae. Moreover, it affected the type 1 fimbriae at gene level probably by mutation during initial attachment of biofilm formation.
Common carp ( Cyprinus carpio L.) juveniles averaging 7.68± 0.25 - 8.12± 1.14 g in weight were used for 6 weeks to investigate their survival and growth preferences. The experimented carp were stocked at 12 tanks formerly adjusted with different pH levels values as: 6.0, 7.0, 8.0 and 9.0. Growth and survival of common carp was assessed every Sunday of each week. Growth rates significantly (P < 0.05 ) increased at pH 7 and pH8 respectively. Therefore, the results suggest that the water with pH ranged from 7 to 8 was the best range for growth performance and survival rate of carp. Feed conversion ratio (FCR) improved at pH 6 and 9 respectively. In general, the results indicated that water p
... Show MoreThe Mishrif reservoir (Cenomanian - Turonian) in the Z, H, B and N oilfields in southern Iraq was investigated to clarify how nickel, vanadium, asphaltene, NSO and sulfur content affect the crude oil quality. The GC-Mass and ICP-MS analyses were used to provide fruitful hydrocarbon results. Classification of crude oil based on API gravity broadly indicates the oil's density and general properties. Typically, lighter crude oils are easier to refine, yield higher percentages of valuable products such as gasoline and diesel, and have a higher market value. Heavier crude oils require more processing and may yield more residual products, such as heavy fuel oil and asphalt. The Mishrif crude oil was classified as a medium sour crude oil c
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(3)
Soil defilement with "raw petroleum" is a standout amongst the most across the board and genuine ecological issues going up against both the industrialized and oil country like Iraq. Along these lines, the impact of "raw petroleum" on soil contamination is one of most critical subjects that review these days. The present examination expects to research "unrefined oil"effectson the mechanical and physical properties of clayey soils. The dirt examples were acquired from Al-Doura area in Baghdad city and arranged by the "Brought together Soil Grouping Framework (USCS)" as silty mud of low pliancy (CL). Research center tests were done on contaminated and unpolluted soil tests with same thickness. The dirtied tests are set up by blending
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Recently, Systemic lupus erythematosus (SLE) was considered as one of the autoimmune diseases that the genetic and environmental factors contributed in the disease etiological profile. According to the environmental factors, infectious agents have been concluded to have a role in the etiology and pathogenesis of SLE. Chlamydia pneumoniae and Mycoplasma pneumoniae are among these infectious agents that have been suggested to be involved in the etiology of SLE. Accordingly, the current study was designed to assess the anti-C. pneumoniae and anti-M. pneumoniae IgG antibody status by enzyme linked immunosorbent assay (ELISA) in the sera of 64 Iraqi SLE females' patients and 32 Iraqi healthy females as controls. The patients' group were distribu
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Three-dimensional seismic reflection study was conducted for the Eastern
Baghdad oil field which is located in the middle part of Iraq within Al-Madaaen
province that belong to Baghdad governarate, South of Diyala River , this field
includes two southern parts (S1 and S2), the study area was about 781.905 km 2 for
the upper Cretaceous age, synthetic seismogram was generated from data of EB-5
and EB-1 wells. Saadi reflector was picked and identified to determine the Hartha
Formation. The seismic sections and time slice maps confirmed that the upper
Cretaceous age was affected by faults and the indicators of faults ended within
Hartha Formation and continue to the deeper formations with increasing intensity.
The
(4)
The present research includes the effect of different concentrations of NaCl on some biological aspects (longevity, growth and molting) of Acanthocyclops bicuspidatus (Copepoda; Cyclopoida). The results showed a decrease of longevity with the increase of salt concentrations. The lowest mean of male′s longevity was 6.8 day in the 2ppt concen. compared with the control treatment 34.2 day, The lowest mean of female′s longevity was 5.1 day in the 2ppt concen. compared with the control treatment 30.1 day. The mean growth for males was lower than the mean growth of females. In males the lowest m
... Show MoreIn this study, the number of times of heating of olive oil was studied. For the different refineries, the study was conducted using a temperature of 300K and for several times to determine the validity of the olive oil which is used for frying purposes after it was used for more than once times. It was found that there were significant changes in the behavior of the photophysical behavior like absorption and emission; there are changes in the nature and composition of the olive oil molecule, which makes it not useful for human use. The number of heating times was ten. The heating times also showed significant changes in the nature of the oil, such as color, taste, density, and smell.
The effects of essential oilNigella sativa and Menthawas study on the chemical, microbial and sensory properties for soft white cheese that produced from it during storage at 0, 7 and 14 days .The results show significantly percent decrease in moisture for all samplesand maximum decrease was at the latest storage period for all them .The reduced in moisture was accompanied with increase in percentage of protein and fat during of storage period for all samples.
The control sample showed increased in bacterial logarithmic for total count bacterial, coliform, Staphylococcus aureus, proteolytic bacteria, lipolytic bacteria and mold and yeasts during of storage period , the highest results showed at the latest storage period 14days, it w
Stable new derivative of L-ascorbic acid, 5,6-O-iso propylidene 2,3-O,O acetic acid-Lascorbic acid (L) was synthesized in good yield by the reaction of 5,6-O-isopropylidene-Lascorbic acid with chloroacetic acid in presence of potassium hydroxide. The new product (L) was characterized by 1H, 13C–NMR, mass spectrum and fourier transform infrared (FTIR). The reaction of the ligand (L) with metal bivalent ion., M+2 = (Co, Ni, Cu, Cd, Hg, Mg, Ca, Pb) synthesized and characterized by FTIR, UV-Visible, Molar conductance, Atomic absorption and the molar ratio (Ni+2, Cd+2) complexes. Spectroscopic evidence showed that the binding of the M(II) ions with (L) are through the (C–I=O) Lacton and O-2-CH2COO– as a bidentate manar re
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