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The Biological Effect of Rosmarinus officinelis L. Essential Oil on Biofilm Formation and Some Fimbrial Genes (fimH-1 and mrkD) of Klebseilla pneumoniae
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Klebseilla pneumoniae possesses many virulence factors and survival strategies to persist and overcome host defenses; one of these strategies is biofilm formation. Therefore, the aims of this study was to determine the antibacterial and antibiofilm effect of Rosmarinus officinelis L. essential oil (EO) and its effect on the genes encoding of fimbrial adhesions. The antimicrobial activity was investigated by MIC. The ability to form biofilm as well as inhibition of initial cell attachment and biofilm formation was performed. PCR was carried out to detect fimH-1 and mrkD genes of type 1 and type 3 fimbrial adhesions at different time of incubation. The study revealed that MIC value of EO was 104 μg/ml on 24 (83%) of isolates, 93% of them produced biofilm. Fifty percent reduction in biofilm formation was observed in 10% of isolates at concentration 104 μg/ml and increased to 45% when used 1.5×104 μg/ml of EO. PCR product of fimH-1 was detected at 24 h but absence at 0 and 4 h while mrkD product found in all incubation time. In conclusion, Rosemary EO had antibacterial and antibiofilm activity against Klebsiella pneumoniae. Moreover, it affected the type 1 fimbriae at gene level probably by mutation during initial attachment of biofilm formation.

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Publication Date
Sat Jan 01 2022
Journal Name
Indian Journal Of Ecology
Effect of Mycorrhiza, Cytokinin and Organic Fertilizer on the Growth and Yield of Chamomile Plant L)
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The experiment was carried out in College of Agricultural Engineering Sciences, University of Baghdad during November 1, 2019 to June 1, 2020. The experiment was designed according to a randomized complete block design. Each block contained 18 experimental units that included the three study factors. The first factor interaction between two inoculum densities and application methods, three levels control, treatment of seeds inoculation with 5g plant and treatment of seedlings inoculation whith 30g plant . The second factor was three -1 -1concentrations of spraying of kinetin 0, 75 and 150 mg L , and third factor include two levels of organic manure) and addition of fertilizer 2% of -1 the weight of the soil (O2). The results showed a signif

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
THE EFFECT OF CHEMICAL AND BIOLOGICAL TREATMENTS ON IMPROVING THE NUTRITIVE VALUE OF CORN COBS AND WILD REED
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This study was conducted in Animal Resources Department , College of Agriculture to estimate the effect of chemical and biological treatments to improve the nutritive value of poor quality roughages ( corn cobs and wild reed ) . The feeds were treated chemically with 4% NaoH solution ,whereas Aspergillus niger was used to ferment corn cobs and wild reed samples . The chemical analysis showed that protein percentages of corn cobs and wild reed was increased significantly (P<0.05) from 6.05% to 10.51% and 17.70% and from 3.10 %to 6.50% and 9.96% for both chemical and biological treatments respectively. The crude fiber percentages decreased significantly (P<0.05) from 29.19% and 26.10% to 23.60% and 20.10% for chemical treatment and was 20

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Publication Date
Tue Apr 22 2014
Journal Name
International Journal Of Advanced Research
Effect of tillage system and deficit irrigation on consumptive use, some soil physical properties, growth and yield of potato Solanum tuberosum L.
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Publication Date
Thu Oct 01 2020
Journal Name
Biochemical & Cellular Archives
THE STUDY ON ABILITY OF ESCHERICHIA COLI ISOLATED FROM DIFFERENT CLINICAL CASES TO BIOFILM FORMATION AND DETECTION OF CSGD GENE RESPONSIBLE FOR PRODUCE CURLI (FIMBRIAE)
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A total of 165 clinical sample included Urine, Swab wounds and Burns were collected from Baghdad Governorate. Results showed that rate all isolates of E. coli was 50(30.3%) and rate of urine infection was 46(92%) and rate of swab wounds infection 4(8%). Where was diagnostic based on streaked on MacConkey agar, then single colony was transferred to Eosin Methylene Blue (EMB). Identification some of the biochemical test included: Catalase test, Oxidase test, Indole test, Methyl red, Vogues - Proskauer test and Citrate Utilization test. Then confirmed by the Vitek - 2 Compact System. The ability of E.coli isolate to biofilm formation to be studied it is considered one of the most important factors of virulence and has role in causing injury an

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Scopus
Publication Date
Wed Jan 01 2020
Journal Name
Plant Archives
The effect of adding some essential oils to the physicochemical properties of frozen beef sausage with different storage periods
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Scopus (1)
Scopus
Publication Date
Wed Jul 01 2020
Journal Name
Plant Archives
THE EFFECT OF ADDING SOME ESSENTIAL, OILS TO THE PHYSICOCHEMICAL PROPERTIES OF FROZEN BEEF SAUSAGE WITHI DIFFERENT STORAGE PERIODS
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RMK Al-Zaidi, MM Ahmed

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Influence of the Surrounding Plants by Rapeseed Field on Population Density of Cabbage Aphid (Brevicoryne brassicae L.) and its Biological Enemies
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The result revealed that the peak of population density of cabbage aphid Brevicoryne brassicae was 523.20 individuals/plant on 21 March in edges of rapeseed field and was 1141.67 individuals/plant in center of the field. Results revealed that population density of cabbage aphid in rapeseed fields surrounded by cover crops significantly were low compared with that of monoculture rapeseed. The location of rapeseed plants (in edges or in center) significantly affected (p<0.05) the tested pest density, e.g. optimum density was 146.69 individuals/plant in the center of the field. Whereas was 93.32 in the edges. Effect of the interaction between location and surrounding vegetation was significant on aphid density, which their population densit

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Publication Date
Mon Apr 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Effect of Extract of Nerium oleander L. Leaves and Proline Acid on Growth and Yield of Chict (Cicer aeitium L.)
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stract The experiment aimed to study the effect of different concentrations (10 and 20)% of Nerium oleander L. leaves extract and proline (20 and 30) ppm on growth and yield of Cicer aeitium L. The field experiment was conducted during the growth season of 2012-2013. The results showed that the best concentration of N. oleander extract leaves was 10% that showed significant increasing in plant height, branches and leaves number and dry weight during first and second periods measurement as well as the chlorophyll containing in leaves , crop growth ratio (CGR), number of pods, weight of 100 seeds, percentage of seed carbohydrates. The results revealed that two concentrations of proline (20 and 30) ppm showed a significant increase in the c

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Determination of Oil Biodegradation Activity of Pseudomonas aeruginosa Isolated from Soil and Molecular Detection of Responsible Genes
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This study concerns the isolation of oil degraded bacterial samples from oil polluted soil in Al-Dora refinery/ Baghdad – Iraq. Soil samples (15) were on mineral salt agar medium (MSM) used to screen the oil degrading bacteria by forming clear zones around the colonies. To confirm the degradation of oil by these bacteria, the isolates were inoculated in mineral salt broth, 15 isolates of Pseudomonas spp. was detected from which two isolates identified as P. aeruginosa by morphological, physical and biochemical characteristics that confirmed by using Vitick identification system. Growth was estimated in terms of whole cell by measuring optical density at 620 nm and free extract protein was estimated by protein measurement with Folin phe

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Publication Date
Thu Jun 15 2023
Journal Name
Bionatura
Study the effect of essential oils of some plants in protection from Cowpea beetle, Callosobruchus maculatus in laboratory
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The experiments were conducted in laboratory conditions of a temperature of 25± 2C and relative moisture of 40± 5 % to evaluate the effectiveness of the cinnamon, lavender and clove essential oils on some biological life aspects of cowpea beetle, C. maculates. Results of the obligative experiment for the effect of the oils on insect adult killing showed that the concentration of 5% caused a mortality percentage averaged 13.33% of the insect males. The mortality percentage of the insect females was 11.3% for the cinnamon and lavender oils. The lavender oil had the lowest effect on adult killing, not exceeding 0. For the effect of the oils on egg laying, clove oil affected the number of eggs highly at the concentration of 5%, result

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