The study included selection six species of the fungi related to Pleurotus genus were evaluated for their ability to production of Pleurotin, one of them, Pleurotus ostreatus (P.11) was isolated and identified in the present study. Pleurotin was extracted with screening by Thin Layer Chromatography (TLC) and quantification High Performance Liquid Chromatography (HPLC). Cytotoxicity of Pleurotin extracted from P. ostreatus (P.11) grown in different sugar sources (galactose, mannitol, sucrose, dextrose and lactose) liquid media was test against three selected cancer cell lines, CaSki, MCF-7 and A549 addition to Human Non Cancer Fibroblast Cell Line (MRC-5). Pleurotin of P. ostreatus (P.11) grown in galactose induced the significant highest growth inhibition against all three cancer cell lines MCF-7 CaSki, and A549 at 72 h treatment period with IC50 29.84 ± 2.37, 30.25 ± 2.40 and 37.60 ± 2.65 μg/ml respectively when the P≤0.01, while it showed no adverse effect on the non-cancer human fibroblast cell line MRC-5 with IC50 >200 μg/ml. Cytotoxicity of Pleurotin was compared with cytotoxicity of the positive controls (chemotherapeutic drugs) including Doxorubicin against CaSki and A549 cell lines and Cisplatin against MCF-7 and MRC-5 cell lines, although IC50 of pleurotin was higher (30.25 ± 2.40 and 37.60 ± 2.65 μg/ml) than Doxorubicin (0.18 ± 0.00 and 1.10 ± 0.02 μg/ml) of CaSki and A549 cell lines, respectively, and also IC50 of Pleurotin was higher (29.84 ± 1.73 and >200 μg/ml) than Cisplatin (8.20 ± 0.25 and 8.88 ± 0.13 μg/ml) of MCF-7 and MRC-5 cell lines, respectively, pleurotin was natural product from an edible fungus while Doxorubicin and Cisplatin were chemical drugs.
Toxicity with advanced glycation end products (AGEs) is a major problem in uremic patients. Treatment with peritoneal dialysis (PD) exacerbates AGE formation as a result of bioincompatibility of the conventional peritoneal dialysis fluid (PDF). The presence of glucose degradation products (GDPs) in PDF is the main cause of its bioincompatibility. Carnosine is an endogenous dipeptide with a powerful antiglycation/antioxidant activity. In an attempt to improve PDF biocompatibility, we evaluated the effect of carnosine in human peritoneal mesothelial cells (HPMC) incubated with PDF or GDPs in vitro. Methods: HPMC were incubated for short or prolonged time with PDF in the presence or absence of carnosine. Similarly, HPMC were incubated in the s
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Background: Gugglusterone has been reported to provide protection against inflammatory and oxidative reactions of different pathological conditions. Objectives: The main object of this research work is to evaluate the renoprotective effects of guggulsterone in the prevention of cisplatin-induced nephrotoxicity in rats via assessment of renal function and histological study. Materials and methods: Rats in this study were split into four groups which comprise a control group, an induction group, a third group receiving low-dose guggulsterone, and a fourth group receiving high-dose guggulsterone. Results: a single dose of cisplatin drug has jeopardisedrenal physiology that has been demonstrated in histopathology sections and elevation
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Rosemary (Rosmarinus officinalis L.) is one of the most economically important species of the family Lamiaceae. Rosemary extract was examined by applying 2.2-diphenyl-1- picrylhydrazyl (DPPH) radical-scavenging assays. The result proved that rosemary extract had a higher antioxidant activity by absorption at a wavelength of 517a nm by using three different concentrations (0.5, 1.0 and 3) mg/ml which performed the absorbance at (2. 314, 0. 211 and 0.296) nm in comparison with control (21.8, 92.2 and 90) nm respectively. Results obtained using chemical detection of the phytochemicals indicated the presence of flavonoids, phenols, saponins, Steroids and cardiac in rosemary water extract. Water extracts of R. officinalis leaves were inves
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The aim of this study was toward the possibility of producing antigen that has the ability to stimulate the immune response against the infection with the hydatid cyst. To do so antigens were extracted from sheep hydatid cyst fluid of Echinococcus granulosus .These were: 1- The hydatid cyst fluid called antigen B. 2- Excretion-secretion called ES antigen. 3-B/ES antigen is a mixture (1:1) of the above two antigens. Three concentrations (15, 30 and 60 µg/ml) from antigen B/ES were prepared to immunize the white mice (males) with 20 µg/gr body weight and one booster dose (10 µg/gr) to stimulate immunity. The efficiency of t
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Leishmania are protozoan parasites belonging to the family Trypanosomatidae that cause high morbidity and mortality levels with a wide spectrum of clinical syndrome. This study aimed to investigate the effect of liposomal amphotericine B (AmBisome) drug on promastigote and axenic amastigote stages of Leishmania donovani isolate (MHOM/IQ/2005/MRU15)in comparison with pentostam SbV drug. Different concentrations of AmBisome and SbV drugs were investigated against Leishmania donovani promastigote and axenic amastigote. The ICR50R values of SbV and AmBisome drugs on promastigote were10.12 mg/ml and 2.21μg/ml, respectively, while they were 0.77μg/ml for axenic amastigote for both drugs. The present study concluded that axenic amastigote was
... Show MoreLeishmania are protozoan parasites belonging to the family Trypanosomatidae that cause high morbidity and mortality levels with a wide spectrum of clinical syndrome. This study aimed to investigate the effect of liposomal amphotericin B (AmBisome) drug on promastigote and axenic amastigote stages of Leishmania tropica. From the 20 isolates of cutaneous leishmaniasis collected from patients attended to the AL-Karama Teaching Hospital in Baghdad during the period from October 2013 until February 2014, only three isolates successfully transformed to motile promastigote stage in the culture media. The most active one is included in this study. Different concentrations of liposomal amphotericin B (AmBisome) and pentostam Sb (V) drugs were inv
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