The aims of this study were to prepare Cryptococcus neoformans killed whole cell antigen and evaluate their effects by using DTH- skin test and the differential white blood cells in local rabbits.
Fourteen animals of both sexes were divided randomly into two groups. The first group (7 animals) was immunized with 1 ml of Cryptococcus neoformans killed whole cell antigen (1x 10 cells/ml), subcutaneously. A booster dose was given after 14 days of the first dose. The second group (7 animals) was considered as control group. Based on results of DTH-skin test, no significant differences (P< 0.05) were recorded between the concentrations, 15 mg/ml and 7.5 mg/ml after 24, 48 and 72 hrs., but there was a significant differences (P<0.05) between these concentrations and 7.5 mg/ml and 3.75 mg/ml and control site. There was no significant difference between all types of cells (neutrophils, lymphocytes, monocytes, and eosinophils) of immunized and control groups.
Back ground: Visceral leishmaniasis is an endemic protozoan disease in Iraq. Recovery from this disease confers a solid and permanent immunity. Immunological assessment of our patients was carried out and the results showed a significant reduction in the percentage of CD3, CD56 and a significant increase in the percentage of CD19 in the peripheral blood lymphocyte of VL in comparison with control group.
Patients and methods: Indirect immunofluorescence technique analysis was performed to detect the percentage of CD3, CD19and CD56 positive lymphocytes.
Results: Our results in the patients groups showed decrease in the percentage of CD3, CD56 and increase in the percentage of CD19. Follow up of patients after
Out of 1279 stool sample only 245 were found to be infected with E.histolytica with total percentage 19% . Diagnostic study for E.histolytica by using techlab Eliza test showed that the non-pathogen E.dispar was significantly higher (78.9%) than E.histolytica (22%).We test the effect of the attenuated E. histolytica by gamma Radiation on the mortality rate in laboratory animals infected with E. histolytica by using increasing doses of radation (5,10,15,20and 25) Rad., the results showed that the percentage of mortality decrease when increase the dose of attenuated E.histolytica cyst in comparison with positive control group (non – attenuated group) it reached (100% ,66.7%, 33.3% 0% and 0%)respectively. Also we found that gamma radiatio
... Show MoreBreast cancer (BC) is the most commonly diagnosed cancer in women. The metabolism of iron is closely regulated by hepcidin which exerts its action by interacting with a ferroportin.
The aim of the present study was to assess the alterations in the levels of some serum biomarkers that have a role in iron homeostasis (hepcidin and ferroportin) in addition to hematological parameters (hemoglobin, leukocyte and platelets count) in different stages of BC.
This study included 66 women with BC. The patients were categorized as follows : group 1 includes :22 patients with stage I disease ,group 2 includes: 22 patients with stage II disease ,and group 3 include: 22 patients with stage III disease .Group 4 includes :22 appare
... Show MorePregnancy-induced hypertension (PIH) is a major pregnancy complication that causes pregnant women mortality. Here, we had scrutinized the correlation between serum levels of -hCG and biochemical parameters in PIH. Serum samples were collected from eighty Iraqi women (forty women with pregnancy-induced hypertension as patients group, twenty normotensive pregnant women as a positive control, and twenty normotensive non-pregnant women as a negative control) all groups were diagnosed clinically. All -hCG hormone level was measured for all studied groups, also serum uric acid, albumin, and total protein levels were measured as biochemical parameters. Data analysis showed that serum level of -hCG hormone was significant increase (p≤
... Show MorePre-eclampsia complicates 2-8% of all pregnancies and it is one of the leading causes of maternal mortality and pre-term delivery in the world. Unfortunately, there is scarcity of documents discussing the circulating level of several essential trace elements in Pre-eclampsia patients in Baghdad especially in the last trimester of the pregnancy. The present study was designed to quantitative evaluation the whole blood concentration of two trace elements, copper (Cu), and iron(Fe), in pre-eclamptic women in the third trimester of pregnancy. The study was conducted on 18 Pre-eclamptic pregnant women as patients group with clinical detected high blood pressure ≥140/90 mmHg and 13 normotensive pregnant women as control group from Al-Alwiya
... Show MoreIn this study, the attention was focused on the protective role of seeds oil from local Onopordum acanthium L. (cotton thistle) against tissues damage in the liver, kidney and spleen in male albino rats. Forty adult male rats were randomly divided into four equal groups including control group, rats were treated orally with seeds oil (0.5ml/kg), carbon tetrachloride (CCL4) injected group, and last group was intoxicated with CCL4 and daily treated with seed oil (0.5ml/kg). After four weeks of the experiment, rats were anaesthetized and blood was taken directly by cardiac puncture for the evaluation of studied parameters. Samples of liver, kidneys and spleen were fixed in 10% formalin for histological studies
... Show MoreCarbamazepine (CBZ) is a narrow therapeutic index drug used in the treatment of trigeminal neuralgia and psychiatric disorders. Valerian (VAL) is a popular herbal product which should be prescribed to treat insomnia and anxiety. The study was designed to investigate the presence of significant pharmacokinetic (PK) interaction between Valerian (VAL) at different concentrations on Carbamazepine (CBZ) pharmacokinetic parameters in healthy male rabbits. In an in vivo, parallel-randomized controlled trial, the rabbits in three groups "first (control), second and third" were given oral doses of CBZ (50 mg/kg), for "second and third" groups (as test groups) rabbits were given (20 and 40 mg/kg/day) of the VAL respectively, as suspensi
... Show MoreThe effect of myeloid leukemia, especially cute myeloid leukemia (AML), has been widely noticed on the parameters of liver and kidney functions and the levels of certain hormones. This study aimed to evaluate a number of biochemical parameters of liver and kidney functions and hormones in Iraqi subjects with newly diagnosed acute myeloid leukemia. Eighty newly diagnosed AML adult patients (40 males and 40 females) and forty healthy individuals (20 males and 20 females) with an age range of 16-75 years were involved in this study during their attendance at the Hematology Department of Baghdad Teaching Hospital/ Medical city in Baghdad province from March 2019 to February 2020. Blood samples were collected from all subjects for
... Show MoreIn recent years, the consideration of natural products as anti-inflammatory and antioxidative treatments has more interested worldwide. Moreover, natural products are easily obtained and are relatively safe the Royal jelly (RJ) is one of them. The current study was carried to evaluate the effects of pregabalin (PGB) on physiological activity of sperms, reproductive hormones assay and some biochemical analysis. Forty (40) male albino rats (10-weeks-old) were divided into four groups (10 rats each): G1 (treated with PGB drug, 150 mg/kg B.wt (Lyrica-Pfizer-Pharmaceutical Industries), G2 (treated with RJ 1g/kg), G3 (treated with PGB drug and RJ together), and G4 control treated with norma