The aims of this study were to prepare Cryptococcus neoformans killed whole cell antigen and evaluate their effects by using DTH- skin test and the differential white blood cells in local rabbits.
Fourteen animals of both sexes were divided randomly into two groups. The first group (7 animals) was immunized with 1 ml of Cryptococcus neoformans killed whole cell antigen (1x 10 cells/ml), subcutaneously. A booster dose was given after 14 days of the first dose. The second group (7 animals) was considered as control group. Based on results of DTH-skin test, no significant differences (P< 0.05) were recorded between the concentrations, 15 mg/ml and 7.5 mg/ml after 24, 48 and 72 hrs., but there was a significant differences (P<0.05) between these concentrations and 7.5 mg/ml and 3.75 mg/ml and control site. There was no significant difference between all types of cells (neutrophils, lymphocytes, monocytes, and eosinophils) of immunized and control groups.
Study Some Immunological Parameters in Rabbits Immunized with Cryptococcus neoformans
Cryptococcus neoformans
Local rabbit
Immune response
Experimental infection
Publication Date
Tue Nov 20 2018
Journal Name
Infection And Immunity
Acknowledgment of ad hoc reviewers
EDITORIAL
The editors of Infection and Immunity are grateful to the following individuals
who provided reviews during the period from October 2017 through September 2018. Thank you for your invaluable support.
Publication Date
Sat Oct 01 2022
Journal Name
Environmental Advances
Stability and performance studies of emulsion liquid membrane on pesticides removal using mixture of Fe3O4Â nanoparticles and span80
The current study investigated the impact of nonionic surfactant span 80 in the existence of Fe3O4 magnetic nanoparticles on the emulsification of mixture of kerosene as petroleum based organic solvents and corn oil as a green diluent in the ratio 1:1
HCl was used as internal phase and the stability of the emulsion was carried out. The proposed Pickering emulsion liquid membrane has been exploited to investigate it is ability in the extraction of Abamectin pesticides from aqueous solution without utilizing carrier agent. Further
the effects of experimental parameters on extraction efficiency and emulsion stability such as
homogenizer speed
emulsification speed
contact time
Fe3O4-Span 80 ratios
HCL concentration
internal to membrane volume ratio (I/O) and pH of the external feed solution were carried out. The results showed that more than 99% of Abamectin could be extracted at 10 min contact time with a minimum breakage percent of 0.52% at the optimal conditions. The kinetic of the extraction were studied and the mass transfer coefficient was found to be for external phase () of 1.2
interfacial reaction rate constant () of 5.83 and the overall mass transfer coefficient () of 3.91 . The results of recyclability of the PELM revealed that the emulsion stability and extraction efficiency was nearly unchanged after three cycles.
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