The current study aimed to synthesize silver nanoparticles (Ag NPs) in a safe and eco-friendly biological method using green tea extract, characterize them using UV-vis spectroscopy, FTIR, XRD, FESEM, and AFM tools, then investigate their antioxidant potentials against DPPH and their cytotoxicity toward lymphoma cells using MTT and ethidium bromide/propidium iodide dual staining assays, and their genotoxic capacities by assessing their effects on the gene expression of p53, caspase3, bcl-2, and bax genes by qPCR and DNA fragmentation. Results confirmed the synthesis of silver nanoparticles; the UV-vis results showed a peak of absorbance at 414 nm; FTIR analysis showed absorbance belonging to the functional groups of green tea; XRD assay results confirmed the crystallinity of Ag NPs; and AFM revealed a mean diameter of around 24.37 nm. Finally, FESEM showed that the shape of Ag NPs was that of spherical aggregated nanoparticles. The synthesized nanoparticles showed antioxidant activity and cytotoxic effects against lymphoma cells, with an IC50 = 1.138 μg/ml. Cell staining results showed alternations in the shape of treated cells due to apoptosis. Ag NPs affected gene expression as they increased proapoptotic genes p53, caspase3, and bax by 3.3, 2, and 4.25fold, respectively, while decreasing antiapoptotic gene expression bcl-2 by 0.59fold. We concluded that the biological way is an efficient way to synthesize silver nanoparticles, and the synthesized NPs have antioxidant potential and are cytotoxic and genotoxic toward lymphoma cells; thus, they may be utilized in related biomedical applications.
Joints are among the most widespread geologic structures as they are found in most each exposure of rock. They differ greatly in appearance, dimensions, and arrangement, besides they occur in quite different tectonic environments. This study is important because joints provide evidence on what kind of stress produced them (history of deformation) and also because they change the characteristics of the rocks in which they occur. The Measured data of joints from the studied area which are located in the high folded zone – Northeast of Iraq, were classified according to their relationship with the tectonic axes by projecting them stereographically using Schmidt net in GEOrient ver.9.5.0 software. The joint systems revealed the orientation of
... Show MoreMarshlands environment in southern Iraq is unique and is considered a habitat of thousands of migratory birds as shelter and a source of livelihood for thousands of people living there. Its environment is characterized by a fragile ecosystem that requires great care and effort to achieve the greatest possible balance and parallelism of development, which necessarily require careful environmental planning that accurately regulates the resources of the environment and therefore, planned the best way to use them. The idea of research for creating the spatial organization of the development of the human settlements and taking into account the environmental aspect by thinking for the plann
Optic Disc (OD) localization is a basic step for the screening, identification and appreciation of the risk of diverse ophthalmic pathologies such as glaucoma and diabetic retinopathy.In fact, the fundamental step towards an exact OD segmentation process is the success of OD localization. This paper proposes a fully automatic procedure for OD localization based on two of the OD most relevant features of high-intensity value and vasculature convergence. Merging ofthese two features renders the proposed method capable of localizing the OD within the variously complicated environments such as the faint disc boundary, unbalanced shading, and the existence of retinal pathologies like cotton wall and exudates,which usually share the same
... Show MoreThe reticuloendothelial system (RES) play an important role in immunity against bacterial infection and Klebsiella pneumoniae one of the most common causes of hospital-acquired infections. Dextran70 (D70), a polysaccharide, may alter functions of this system through changing many biological activities in the tissues.
The media guide is a small-sized printed medium, whose importance is highlighted through the specific information it carriesThe task of the guide lies in the clarity of these indications and making a positive impact on the recipient with understanding so it is a means for the purpose of reaching the effect and effectiveness of the message symbols from the movements of lines in which elements that are supposed to be equal in the performance of their functions and the purpose of their existence gather to achieve the mental response of perception, understanding and enjoyment. It must be in an objective unity based on the balance in this distribution. To form a formal organizational unit and an integrated system, the result of which is to gi
... Show MoreThe acute phase response is a major pathophysiologic phenomenon that accompanies inflammation whether acute or chronic. Complement (C3 and C4) and C - reactive protein (CRP) are positive acute phase proteins (+ ve APPs ). Their production takes place in hepatocyte and the blood concentration of these parameters are increased in osteoarthritis (OA). Chloroquine (CQ) is a diprotic weak base traditionally used to treat malaria. Recently the phosphate salt of CQ is used to decrease this type of (+ve APPs) . In this study, patients who suffered from knee osteoarthritis (KOA) are treated with oral dosage form of chloroquine phosphate (CQP) for one month, twice daily. Our results demonstrate that CQP improves the patient status by decreas
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