According to the high operational costs, low stability, and reusability of enzymes, immobilization by nanoparticle gathering has increased in recent years. Iron oxide nanoparticles (magnetite nanoparticles, Fe₃O₄) have been prepared by mixing one volume of iron dioxide ions and two volumes of iron trioxide ions with HCl via the precipitation of iron salts by NH₄OH. The features of magnetic nanoparticles have been studied by Atomic Force Microscopy (AFM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy (SEM). The prepared Fe3O4 was used in the adsorption method to immobilize the galactosidase enzyme. The immobilized enzyme has been compared with the crude one by optimizing time, PH, temperature, substrate solution concentration, and enzyme solution concentration. As a result, the immobilized enzyme has exhibited higher activity over long storage times, and a wide PH and temperature range than crude enzyme. In addition, whatever the substrate and enzyme concentrations, the activity of the immobilized enzyme increased over the non-immobilized one. As a result, a lower Km value for immobilized D-galactosidase indicates a higher affinity for the substrate than crude D-galactosidase.
Background: It is well known that the low level laser irradiation has act on immune system cells in a number of ways, one of them includes increasing phagocytic activity of irradiated cells. This study was established to shed light on the possible effect of laser irradiation on phagocytic activity of polymorphoneutrophils.
Subjects and methods: Fresh blood samples were obtained from twenty healthy volunteers for phagocytosis assay. The polymorphoneutrophils were isolated from blood and examined their phagocytic capacity befor and after exposure to laser irradiation.
Results: The present study revealed a significant increase in the mean percentage of phagocytosis after exposure to diode laser of wave le
The aim of this study was to know the inhibition activity of squeezed grape waste extract on Bacillus stearpthermophilus by using three different tempretures degree 40, 60 and 80c, in order to reduce the time exposure of food for preservation. This study include two branchs: First: isolation and identification of Bacillus stearothermophilus from soil, 5 sample were collected from the soil of the college agriculture/Baghdad university. Samples were cultured on nutrient agar, microscopic and culturing tests were conducted and many biochemical tests were done. The isolates were cultivated at 55 c and 65 c for differentiate it from Bacillus coagulans which is can't grow at 65 co. The c
... Show MoreSome Results on Fuzzy Zariski
Topology on Spec(J.L)
Nowadays, people's expression on the Internet is no longer limited to text, especially with the rise of the short video boom, leading to the emergence of a large number of modal data such as text, pictures, audio, and video. Compared to single mode data ,the multi-modal data always contains massive information. The mining process of multi-modal information can help computers to better understand human emotional characteristics. However, because the multi-modal data show obvious dynamic time series features, it is necessary to solve the dynamic correlation problem within a single mode and between different modes in the same application scene during the fusion process. To solve this problem, in this paper, a feature extraction framework of
... Show MoreDeveloping a solid e-voting system that offers fairness and privacy for users is a challenging objective. This paper is trying to address whether blockchain can be used to build an efficient e-voting system, also, this research has specified four blockchain technologies with their features and limitations. Many papers have been reviewed in a study covered ten years from 2011 to 2020. As a result of the study, the blockchain platform can be a successful public ledger to implement an e-voting system. Four blockchain technologies have been noticed from this study. These are blockchain using smart contracts, blockchain relying on Zcash platform, blockchain programmed from scratch, and blockchain depending on digital signature. Each bl
... Show MoreNitrogen (N) is a key growth and yield-limiting factor in cultivated rice areas. This study has been conducted to evaluate the effects of different conditions of N application on rice yield and yield components (Shiroudi cultivar) in Babol (Mazandaran, Iran) during the 2015- 2016 season. A factorial experiment executed of a Randomized Complete Block Design (RCBD) used in three iterations. In the first factor, treatments were four N amounts (including 50, 90, 130, and 170 kg N ha-1), while in the second factor, the treatments consisted of four different fertilizer splitting methods, including T1:70 % at the basal stage + 30 % at the maximum tillering stage, T2:1/3 at the basal stage + 1/3 at the maximum ti
... Show MoreThe experiment was conducted to study the effect of leaves extract of Salvia sclarea , Rosmarinus officinalis and Thymus vulgaris with 10% and 30% concentration on germination of seeds and growth of seedlings . The effect of these extracts on infection percentage of seeds decay and surface growth of Rhizoctonia solani . The results showed that the three extracts effected significantly to reduced percentage of seeds germination, acceleration of germination , promoter indicator , infection percentage of seeds decay and surface growth of R. solani especially in 30% concentration .
In the current research, the work concentrated on studying the effect of curvature of solar parabolic trough solar collector on wind loading coefficients and dynamic response of solar collector. The response of collector to the aerodynamic loading was estimated numerically and experimentally. The curvature of most public parabolic trough solar collectors was investigated and compared. The dynamic response of solar collector due to wind loading was investigated by using numerical solution of fluid-structure interaction concept. The experimental work was done to verify the numerical results and shows good agreement with numerical results. The numerical results were obtained by using finite element software package (ANSYS 14). It was found
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