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Potentials of Iron Oxide Nanoparticles (Fe3O4): As Antioxidant and Alternative Therapeutic Agent Against Common Multidrug-Resistant Microbial Species

     For the treatment of pathogenic bacterial infections, multidrug resistance (MDR) has become a major issue. The use of nanoparticles is a promising strategy for combating medication resistance in a variety of pathogens that cause deadly diseases. The goal of our research was to extract multidrug-resistant bacteria from wound infections and then use iron oxide nanoparticles (Fe3O4) as alternative therapeutic agents in vitro. Gram staining, morphological attributes evaluation, and biochemical testing were used to assess the microbes. The Kirby-Bauer disk diffusion method was used to test MDR-bacterial strains against several antibiotics; the majority of these isolates were resistant to ceftazidime, amoxicillin, Gentamicin, and tetracycline. the iron oxide nanoparticles were produced by the co-precipitation method and were confirmed by changing the color to dark black as well as the Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) analysis that shows the shape and average size between (29.03-56.54) nm. The highest effect of iron oxide nanoparticles (Fe3O4) on the growth of Proteus.mirabilis (P.mirabilis) was as it was found that the average diameter of the inhibition zone was 22.66±1.15 mm, followed by Staphylococcus.epidermidis (S.epidermidis), Acinetobacter.baumannii (A.baumannii) with the average diameter of the inhibitory zone it was 21.66±1.52 mm, 20.33±1.53 mm respectively, and Candida.albicans (C.albicans) was 18.33±1.15 mm at 100 µgmL-1 (stock). The synthesized iron oxide nanoparticles (Fe3O4) are used to capture rapidly microbes under the magnetic field effect. The antioxidant activity DPPH of the iron oxide nanoparticles (Fe3O4) showed 29.3%, 42.2%, 58.6%, 67.4%, and 74 % at a concentration (6.25, 12.5, 25, 50, 100) µgmL-1 respectively, it demonstrated that the scavenging percentage increase with increasing the iron oxide nanoparticles (Fe3O4) concentrations.

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Publication Date
Fri Jun 28 2019
Journal Name
Bioscience Research
Publication Date
Wed Nov 08 2023
Journal Name
Technologies And Materials For Renewable Energy, Environment, And Sustainability: Tmrees23fr
Determination microstructure parameters for copper oxide nanoparticles

In this research, the results of x-ray diffraction method were used to determine the uniform stress deformation and microstructure parameters of CuO nanoparticles to determine the lattice strain obtained and crystallite size and then to compare the results obtained by two model Halder Wagner and Size Strain Plot with the results of these methods of the same powder using equations during which the calculation of the size of the crystallite size and lattice strain, It was found that the results obtained the values of the crystallite size (19.81nm) and the lattice strain (0.004065) of the Halder-wagner model respectively and for the ssp method were the results of the crystallite size (17.20nm) and lattice strain (0.000305) respectively. The sa

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Publication Date
Fri Mar 01 2024
Journal Name
Baghdad Science Journal
Biosynthesis, Characterization, Adsorption and Antimicrobial studies of Manganese oxide Nanoparticles Using Punica Granatum Extract

Manganese sulfate and Punica granatum plant extract were used to create MnO2 nanoparticles, which were then characterized using techniques like Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy, atomic force microscopy, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The crystal's size was calculated to be 30.94nm by employing the Debye Scherrer equation in X-ray diffraction. MnO2 NPs were shown to be effective in adsorbing M(II) = Co, Ni, and Cu ions, proving that all three metal ions may be removed from water in one go. Ni(II) has a higher adsorption rate throughout the board. Co, Ni, and Cu ion removal efficiencie

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Publication Date
Sat Feb 14 2015
Journal Name
Global Journal Of Advanced Engineering Technologies And Sciences
EVALUATION OF ANTIBACTERIAL ACTIVITY OF METAL NANOPARTICLES AGAINST DIFFERENT PATHOGENIC BACTERIA

In recent years, infectious diseases are increasingly being encountered in clinical settings. Due to the development of antibiotic resistance and the outbreak of these diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and the researchers are now searching for new unconventional antibacterial agents. Recently, in this field, the application of nanoparticles is an emerging area of nanoscience and nanotechnology. For this reason, nanotechnology has a great deal of attention from the scientific community and may provide solutions to technological and environmental challenges. A common feature that these nanoparticles exhibit their antimicrobial behavior against pathogenic bacteria. In this report, we evaluate

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Publication Date
Fri Oct 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
A Novel Approach of CFIA Technique for Assaying of Furosemide (Sulfa Drug) as Antibacterial Agent in Pharmaceutical and Biological Samples Using Potassium Ferricyanide as Oxidizing Agent

Furosemide drug determination in pharmaceutical and biological urine samples using a novel continuous flow-injection analysis technique that is simple, rapid, sensitive and economical. The complex formed by the reaction of furosemide and O-phenylenediamine with oxidative agent K3[Fe(CN)6] to produce an orange-yellow colored product at 460 nm was the basis for the proposed method.  The proposed method’s linearity ranges (3-100) μg.mL-1and (1-50) μg.mL-1 for CFIA/merging zone methods and batch .The detection limit and Limit of quantification values were 2.7502 μg.mL-1  and 9.1697 μg.mL-1 the relative standard deviation was 0.7143 %, and the average recovery is 98.80%

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Publication Date
Thu Apr 18 2019
Journal Name
Iraqi Journal Of Science
Effect of iron nanoparticles and dry yeast extract on the yield and the productivity of corn (Zea maize L.)

This work was carried out at the Glass House at the Faculty of Agriculture/ Anbar University in the period between 2016 to 2017 Nano-iron and dry yeast extract were added to evaluate the response of maize productivity. Three concentrations of nano-iron, 0, 50, and 100 mg, were sprayed on the leaves The yeast extract of three concentrations 0, 100 and 150 grams per liter were sprayed on the leaves. The results showed the superiority of the treatments that were sprayed with nanoparticles with the highest rate of chlorophyll, the seed protein ratio and the percentage of seed oil. Significant levels were also recorded when spraying the plants with dry yeast extract. The interactions of 100 mg of nanotube and 150 g / l dry yeast extract chara

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Publication Date
Mon Dec 20 2021
Journal Name
Natural Volatiles & Essential Oils
Therapeutic Effects of Allicin against the Diabetes Mellitus Induced by Streptozotocin in Male Rats

This study aimed to see how allicin (45mg/kg BW) affected diabetic Mellitus in male rats (DM). Forty male rats were utilized, and they were split into four groups at random for 42 days. T2 was treated with 45 mg/kg B.W of allicin dissolved in 1 ml of D.W daily and injected with a single dose of sodium citrate buffer (0.5ml Intra-Peritoneal IP), DM was induced in T1 and T2 by injection of a single dose of streptozotocin 50 mg/kg B.W IP, T1 was assigned as a positive control, T3 received 45 mg/kg B.W. of allicin dissolved in 1 ml D.W. every day, and a single dose of sodium citrate buffer was injected (0.5ml IP). When diabetic rats treated with allicin in T2 were compared to diabetic rats in T1, the findings indicated a significant increase (P

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Publication Date
Tue Dec 29 2020
Journal Name
Iraqi Journal Of Science
Isolation of Enterococcus Species from Food Sources and Its Antibacterial Activity against Staphylococcus Aureus

The microbial production of substances that have the ability to inhibit the growth of other microorganisms is possibly the most common defense strategy developed in nature. Microorganisms produce a variable collection of microbial defense systems, which include antibiotics, metabolic by-products, lytic agents, bacteriocins and others. The aim of the present study was to isolate and identify Enterococcus spp. and  its most prevalent species from food samples and determine its antibacterial activity against Staphylococcus aureus isolates. A total of 50 food samples from different sources (dairy products (20 samples) and vegetables and fish (15 samples each)) were collected from different local markets in Baghdad and

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Publication Date
Mon Feb 28 2022
Journal Name
Sains Malaysiana
Green Synthesis of Nickle Oxide Nanoparticles for Adsorption of Dyes

The green synthesis of nickel oxide nanoparticles (NiO-NP) was investigated using Ni(NO3)2 as a precursor, olive tree leaves as a reducing agent, and D-sorbitol as a capping agent. The structural, optical, and morphology of the synthesized NiO-NP have been characterized using ultraviolet–visible spectroscopy (UV-Vis), X-ray crystallography (XRD) pattern, Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) analysis. The SEM analysis showed that the nanoparticles have a spherical shape and highly crystalline as well as highly agglomerated and appear as cluster of nanoparticles with a size range of (30 to 65 nm). The Scherrer relation has been used to estimate the crystallite size of NiO-NP which ha

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Publication Date
Sun Jun 02 2019
Journal Name
Baghdad Science Journal
Stimulation of Macrophage Cells Against Cutaneous Leishmaniasis Using Silver Nanoparticles

Cutaneous leishmaniasis is a disease caused by Leishmania tropica parasite. Current treatments for this parasite are undesirable because of their toxicity, resistance, and high cost. Macrophages are key players against pathogens. Nitric oxide (NO), a molecule produce by immune cells, controls intracellular killing of pathogens during infection. Silver nanoparticles (Ag NPs) demonstrated broad-spectrum activity against various types of infectious diseases. It has the ability to stimulate oxygen species production.  This study aims to analyze the macrophages activation through NO production and estimate the cytotoxicity based on the lactate dehydrogenase (LDH) release upon exposure to L. tropica and

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