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Antibacterial Activity of Silver nanoparticles Against Pathogenic Bacterial Isolates from Diabetic Foot Patients
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     Diabetic foot is a catastrophic complication of diabetes. This study included isolation and identification of three types of bacteria that cause diabetic foot ulcers,  fifty-five isolates of Staphylococcus aureus, thirty-five isolates of Acinetobacter baumannii, and thirty isolates of Serratia marcescens. These isolates were obtained from diabetic foot patients at different private clinics in and around Baghdad and Medical City Hospital. The proportion of male patients was greater than females, and it was noted that the age group (51-68 years) was more ages affected by diabetic foot. These isolates showed high resistance to most of the antibiotics used, Staphylococcus aureus was resistant to Penicillin, Tetracycline, and Ciprofloxacin in the percentage of 100 %, 65 %, and 26 %, respectively. Acinetobacter baumannii, showed high resistance to Penicillin, at a percentage of 80%, and Ciprofloxacin at  60%. Serratia marcescens was resistant to most antibiotics that were used in this study, Tetracycline, Penicillin, Cefotaxime, and Amoxicillin in the percent of (100, 95, 91, 88,70, and 64) %, respectively. The chemical reduction method was used in this study to synthesize silver nanoparticles. The characterization of silver nanoparticles was done by Field Emission Scanning Electron Microscope, and transmission electron microscope, which showed particle sizes of 24.56 to 66.2 nm, which proved that silver nanoparticles had nano size and spherical shape. The result of antibacterial activity of silver nanoparticles and silver nitrate against Staphylococcus aureus bacteria showed the highest effect of silver nitrate than other bacteria tested in this study, the diameter of the inhibition zone reached 15.66mm. Likewise for silver nanoparticles where the diameter of the inhibition zone of highest effect reached 29.33mm for Staphylococcus aureus bacteria. The test of silver nanoparticles' ability to inhibit bacterial growth produced the greatest results for Staphylococcus aureus bacteria, which were inhibited after 60 minutes. Based on these research findings, silver nanoparticles have demonstrated their efficacy in this study against isolated bacteria from diabetic feet.

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Publication Date
Thu Apr 18 2019
Journal Name
Al-kindy College Medical Journal
Congenital Club Foot Treated By Of Ponseti Method : A Short-Term Results
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Background: Congenital club foot is a complex deformity of foot .It is a collection of different abnormalities, with different etiologies. Consequently, Severity varies with   difficulties in evaluating treatment strategies with outcome results. The treatment of congenital club foot remains controversial. Usually, the orthopedist's goal is to obtain anatomically and functionally normal feet in all patients.                                Objective: To asses short term follow up result of conservatively treated club feet in relation to the age

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Publication Date
Sun Mar 01 2020
Journal Name
Indian Journal Of Forensic Medicine And Toxicology,
Effect of algal extracts on the growth of tow bacterial types isloated from pollutants discharge
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Effect of Chlorococcum humicola alcoholic algae extract was studied on the growth of, Pseudomonas aeruginosa, and Klebsiella pneumonia, which were isolated from contaminated water. The extract of Ch. humicola showed a high efficiency in reducing the numbers of the two types of bacteria. . The removal rate of K. pneumonia were 0.0, 48.4 and 57.0, The removal rate of P. aeruginosa were 63.1, 79.8 and 82.9% after24,48, 72 h respectively. The results improved that the K. pneumonia is more sensitive than P. aeruginosa for algae extract concentrations used in study ,and the beast effective time is 24h for the two bacterial species The aim of the study was to eliminate microorganisms using the Alcoholic algae extract. Especially P. aeruginosa and

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Publication Date
Sat Oct 01 2022
Journal Name
The Egyptian Journal Of Hospital Medicine
Detection of Bacterial Resistance Genes from Neonatal’s Incubators Environment at Selected Sites of Baghdad Hospitals
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Publication Date
Tue Jan 01 2019
Journal Name
Veterinary World
Bacterial isolation from internal organs of rats (Rattus rattus) captured in Baghdad city of Iraq
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Aim: Rats are accused in disseminating many zoonotic diseases. This study aimed to isolate and identify bacteria from internal organs of rats captured in Baghdad City, Iraq. Materials and Methods: A total of 120 black rats (R. rattus) were trapped from different areas in Baghdad city. Rats were kept in individual plastic cages for 3 h before euthanizing. Deep pharyngeal swab, intestinal content, urine, and pieces of the liver and spleen, lung, kidney, and brain were obtained aseptically. The specimens were inoculated into peptone water and incubated at 37°C for 24 h for enrichment. A loopful of each specimen was then subcultured onto MacConkey Agar, Blood Agar, and Mannitol Salt Agar. CHROMagar O157 H7 and CHROMagar Listeria were u

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Publication Date
Sun Sep 02 2012
Journal Name
Baghdad Science Journal
Synthesis , Spectroscopic and Antibacterial Studies of Zinc(II) Complexes Derived from Salicylaldehyde, Leucylalanine and Glycylglycine
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Two Schiff base ligands L1 and L2 have been obtained by condensation of salicylaldehyde respectively with leucylalanine and glycylglycine then their complexes with Zn(II)were prepared and characterized by elemental analyses , conductivity measurement , IR and UV-Vis .The molar conductance measurement indicated that the Zn(II) complexes are 1:1 non-electrolytes. The IR data demonstrated that the tetradentate binding of the ligands L1 and L2 . The in vitro biological screening effect of the investigated compounds have been tested against the bacterial species Staphlococcus aureus, Escherichia coil , Klebsiella pneumaniae, Proteus vulgaris and Pseudomonas aeruginosa by the disc diffusion method . A comparative study of inhibition values of

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Publication Date
Sun Mar 02 2014
Journal Name
Baghdad Science Journal
Phenolic Content and Antioxidant, Antibacterial Activities of Ethanolic Extract From Lemon Balm and Oregano Plants
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The antioxidant and antibacterial activities of ethanolic extract and phenolic compounds extract of Lemon balm (Melissa officinalis) and Oregano (Oreganum vulgare) plants were studied; the phenolic content and the relationship between these compounds and the above activities were also investigated. The results showed that the Lemon balm had the highest phenolic content (56.5% mg g) and the phenolic content of Oregano was twice lower than Lemon balm. Lemon balm has the highest antioxidant activity which causes lipid peroxidation inhibition activity of linoleic acid (90.5%), this activity was more than ?-tocopherole antioxidant activity (79.3 %). It was found that the main source of antioxidant activity o

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Publication Date
Thu Oct 01 2020
Journal Name
Biochemical And Cellular Archives
ISOLATION OF SOME PATHOGENIC BACTERIA FROM STUDENTS’ MAKEUP AS A PART OF BIOSAFETY IN THE MEDICAL LABORATORIES
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The bacterial contamination of lipsticks and face cream may become a great important matter in the medical laboratories. The present study was designed to determine the types of bacterial contamination in the face cream and lipsticks of undergraduate students. Also, the study aimed to determine the sensitivity of the isolated bacteria against many antibacterial agents. The study included 190 swabs samples from 190 face cream and lipsticks samples of the females’ students from five departments in the Medical Technology Institute, Almansour, Middle Technical University were collected in February 2018. The swab samples were collected with sterile condition and cultured on enriched Blood agar and MacConkey agar. Serial dilutions were made up

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Publication Date
Tue Dec 05 2023
Journal Name
Baghdad Science Journal
Synthesis and Antibacterial Evaluation for Some New Schiff-bases Derived from P-aminoacetanilide
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Derivatives of Schiff-bases possess a great importance in pharmaceutical chemistry. They can be used for synthesizing different types of bioactive compounds. In this paper, derivatives of new Schiff bases have been synthesized from several serial steps. The acid (I) was synthesized from the reaction of dichloroethanoic acid with 2 moles of p-aminoacetanilide. New acid (I) converted to its ester (II) via the reaction of (I) with dimethyl sulphate in the present of anhydrous of sodium carbonate and dry acetone. Acid hydrazide (III) has been synthesized by adding 80% of hydrazine hydrate  to the new ester using ethanol as a solvent. The last step included the preparation of new Schiff-bases (IV-VIII) by the reaction of acid hydrazide with app

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Publication Date
Tue Dec 05 2023
Journal Name
Baghdad Science Journal
Synthesis and Antibacterial Evaluation for Some New Schiff-bases Derived from P-aminoacetanilide
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Derivatives of Schiff-bases possess a great importance in pharmaceutical chemistry. They can be used for synthesizing different types of bioactive compounds. In this paper, derivatives of new Schiff bases have been synthesized from several serial steps. The acid (I) was synthesized from the reaction of dichloroethanoic acid with 2 moles of p-aminoacetanilide. New acid (I) converted to its ester (II) via the reaction of (I) with dimethyl sulphate in the present of anhydrous of sodium carbonate and dry acetone. Acid hydrazide (III) has been synthesized by adding 80% of hydrazine hydrate  to the new ester using ethanol as a solvent. The last step included the preparation of new Schiff-bases (IV-VIII) by the reaction of acid hydrazide with

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Publication Date
Sun Oct 01 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Immunostimulatory, Antibacterial and antibiofilm activity of purified Donkey colostrums lactoferrin on multidrug resistance Serratia liquefaciens producing Intl gene
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Background: Modern methods of biofilm prevention depended on the utilize of normal foodstuffs can solve antibacterial and antibiofilm problems.
Objective: To purify donkey lactoferrin, and evaluate antibiotic resisted Serratia liquefaciens which producing Intl gene and investigate the inhibitory action of lactoferrin on biofilm and stimulate immune response.
Methods: lactoferrin extracted from donkey milk, and purified by ammonium sulfate and Sephadex chromatography. Antibacterial and antibiofilm activities of lactoferrin on Serratia liquefaciens were assayed, and effect of lactoferrin on the innate immune response of mice was determined.
Results: Lactoferrin contains 9.88% carbohydrates, 128 ppm iron, and molecular weight was 8

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