Ninety five samples were collected from different samples (urine, ear and wounds swaps), from hospitals in Baghdad city. The results of cultural, microscopic , biochemical tests indicated that in urine samples E.coli have high occurrence frequency 19 (47.5%) followed by Proteus mirabilis 18(45%) and Klebsiella species 1 (2.5%), while in wounds samples each of Pseudomonas spp. and Proteus mirabilis 10 (25%) , then followed by E.coli with 5 (12.5%) and Klebsiella species 3 (7.5%). Ear swaps samples revealed that Pseudomonas aeruginosa 7 (46%) was the major bacterium followed by Proteus mirabilis 4(26.6).Sensitivity test against eleven antimicrobial agents was done for all of the Proteus mirabilis isolates (32 isolates). The results displayed that most of the isolates were resistant to Methicillin (96.8%), and Rifampin (93.7%) followed by trimethoprim–sulfamethoxazole (71.8%), chloramphenicol (62.5%), and cefazoline (59.3%). while the most effective antimicrobial agents against P. mirabilis were Imipenem (96.9%), Azetronam (81.3%), Azithromycin (71.9%) ,Ciprofloxacin (69%).Whereas a moderate effect appeared against both gentamycin and tobramycin in a percentage of (53.2 and 62.5) % respectively. More resistant isolate was selected, and lipopolysaccharide was extracted by hot EDTA method and the yield was (051) mg LPS from (22)g dry weight cell of pathogenic P.mirabilis and (95) mg as LPS from (16) g dry weight cell of standard bacteria were obtained. After partial purification ,chemical analysis of crud and partial purified LPS showed that the carbohydrate percentages were (35 , 44.3) % and (49 , 62)%, while the protein percentage (0.98 ,0.1) % and ( 1.3 ,0.1)% for the standard and isolated bacteria respectively ,whereas both extract appeared free from nucleic acid . Molecular weight of LPS was estimated and it was equivalent to (63095 and 70794) Dalton for the standard bacteria and pathogenic one respectively.
The Antimicrobial activity of Probiotic Bifidobacterium sp and Prebiotics : chicory roots ( Hot water extract ) and Inulin ( 10 % ) against some Pathogenic bacteria (Esherichia coli , Proteus mirabilis , Klebsiella sp , Pseudomonas aeruginosa , Serratia marcescens was studied . The combination of Probiotic and Prebiotics (Synbiotic) (Bifidobacterium sp + chicory ) and (Bifidobacterium sp + Inulin ) also tested for their antimicrobial activity against Pathogenic bacteria . Results showed that Bifidobacterium sp had good antimicrobial activity against all the Pathogenic bacteria tested , followed by chicory and inulin . The synergistic inhibitory effect of Synbiotic (Bifidobacterium sp + chicory ) and ( Bifidobacterium sp + inuli
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leishmaniasis is a disease of global concern, Leishmania types and distribution rates vary from country to another. For visceral leishmaniasis serum samples were examined by the kala-azar dipstick rapid test. Meanwhile smears were taken from lesions, air dried, fixed and Leishman stained then microscopically examined for amstigote stage in cutaneous leishmaniasis. From April 2014 to April 2015, 150 samples for cutaneous leishmaniasis (CL) and 108 serum sample for visceral leishmaniasis (VL) from in and out patients in Pediatric, Azadi Teaching and Kirkuk General Hospitals were examined. The incidence of visceral leishmaniasis was very low (2.7%) comparing to that in cutaneous leishmaniasis (64.6%). No significant differenc
... Show MoreBackground: To test effectiveness and safety of topical methotrexate 0.5%gel and to introduce new formula of methotrexate gel using suitable media for delivery.
Patients and Methods: The clinical work was performed at the Department of Dermatology and Venereology in Baghdad Teaching Hospital during the period from January 2008 to October 2008. While preparation of formula was performed in the laboratories of the Department of Pharmacology- College of Medicine-University of Baghdad. Patients were divided in to two groups according to the type of treatment, group (I) 32 patients treated with methotrexate 0.5% gel and group(II) (placebo group) included 33 patients treated with placebo gel.
Results: A tota
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Background:
Bronchiolitis is the first episode of wheezing associated with low-grade fever, rhinitis, tachypnea, and increase respiratory effort in a previously healthy infant during the winter
months. It is the most common disease of the lower respiratory tract.
Methods:
This is a case control study carried out on one hundred infants with a mean age of 3.9 months ± 2.2 months admitted to Children Welfare Teaching Hospital in Medical City –
Baghdad with acute bronchiolitis during the period from 1st January 2006 to 1st April 2006. epidemiological risk factors, clinical presentations, chest X-ray findings, treatment,
complications and outcome were analyzed. Another one hundred infants (age and sex
Al-Yusifia river was assessed at three sampling stations with study period from Autumn 2010 to the end of Summer 2011. The present investigation was carried out on diversity of fungi and bacteria from Al-Yusifia river, Baghdad city. During the study, a total of 12 fungal genus and 6 bacterial genus were isolated during the year seasons. The dominant fungus at the three stations were Penicillium sp., then Rhizopus and Trichophyton megninii while the dominant bacteria was Escherichia coli and Klebsiella sp.
The higher
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In this study, we investigated the prevalence of aminoglycosides modifying enzymes (AMEs)-encoding genes, including aac(3′)-ΙΙ, ant(3′′)-Ι, aph(3′)-VΙ, and aac(6′)-Ιb-cr and their potential effect on the development of resistance to aminoglycosides and fluoroquinolones in clinical isolates of Klebsiella pneumoniae. According to the phenotypic and biochemical characteristics of 150 clinical samples, 50 (33%) isolates were identified as K. pneumoniae. These isolates were collected from different clinical sources, including urine (15, 30%), blood (12, 24%), sputum (9, 18%), wounds (9, 18%), and burns (5, 10%). The minimum inhibitory conce
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ArcHydro is a model developed for building hydrologic information systems to synthesize geospatial and temporal water resources data that support hydrologic modeling and analysis. Raster-based digital elevation models (DEMs) play an important role in distributed hydrologic modeling supported by geographic information systems (GIS). Digital Elevation Model (DEM) data have been used to derive hydrological features, which serve as inputs to various models. Currently, elevation data are available from several major sources and at different spatial resolutions. Detailed delineation of drainage networks is the first step for many natural resource management studies. Compared with interpretation from aerial photographs or topographic maps, auto
... Show MoreOne of the most significant difficulties raised by the phenomenon of multiple nationalities is the designation of the law applicable to the dispute involving a foreign element in which the rules of attribution refer to the application of personal law in matters of personal status, which has resulted in jurisprudence and jurisprudence in this area to several criteria that can be applied to weighting one of the nationalities held by the foreign national, as well as the position of national legislation that adopted the most likely of these standards, It can be embodied in two traditional directions represented by the standards of the judge 's law and the law of actual nationality, while the modern trend is the criterion of functional soluti
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