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Purification of Red Wasp Vespa orientalis and Yellow Wasp Polistes olivaceaus Toxin and Estemating Some of its Biological Characteristics

This study included the collecting of 390 gland toxicity of the Red Wasp Vespa orientalis and 418 gland toxicity of yellow wasp Polistes olivaceaus from different areas of Baghdad city. It was classified depending on taxonomic keys in order to diagnose and identify the general characteristics of each species, and then extract gland toxicity to get poison. The poison was purified by gel filtration throw Sephacryl S-200 obtain three peaks of proteins. The activity of lysis and clotting was estimated for peaks separate P1, P2 and P3 for wasp red toxin, the lysis activity of separate P1 was 98% of the poison origin activity and the clotting time was 47.3 minutes, while when estimating the activity of lysis and clotting for peaks separate P1, P2 and P3 for wasp yellow toxin, the lysis activity of separate P2 was 91% of the poison origin activity and the clotting time was 43.8 minutes. The molecular weight of P1 and P2 wasp was 22387 and 22382 Da respectively by using the gel filtration Sephacryl S-200, while the carbohydrates content were 60.057 and 44.460 mg/ ml respectively, The percentage of iron was 0.88 and 0.44 ppm respectively, the optimum pH of lysis and clotting activity for P1 and P2 was 6.5, and noted that they stable at range 6-7.5 and 5.5 -8 respectively, the optimum temperature of lysis and clotting activity for P1 and P2 was 35oC, and noted that they stable at 35oC for 30 minutes.

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Publication Date
Sat Jun 01 2024
Journal Name
Russian Journal Of Bioorganic Chemistry
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Publication Date
Thu Oct 01 2020
Journal Name
Pakistan Journal Of Medical And Health Sciences
Relation of red cell width diameter with some electrophysiological parameters of symptomatic sinus node patients in Iraq(Article)

Background: Dysfunction of sinoatrial node is a set of abnormal rhythms which are resulted from the sinoatrial node malfunctionof the sinus node, the chief natural cardiac pacemaker. The common, and occasionally, the single method for treatment of heart arrhythmias wasimplantation of pacemaker, which reduce symptoms exactly occurs after implantation. Aim: To detect the association between red cell width diameter (RDW) and some cardiac electrophysiology parameters in sinus node dysfunction in Iraqi patients such as SNRT and AH. Methods: A cross sectional study, was conducted on 59 patient ranging between 20-50 years old and involving 35 female and 24 male patients, suffering from an unexplained symptoms of sinoatrial node dysfunction (SND).

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Assessment of risk factors for myocardial infarction and its relationship with some variables

The aim of the study is to assess the risk factors which lead to myocardial infarction and relation to some variables. The filed study was carried out from the 1st of April to the end of Sept. 2005. The Sample of the study consisted of (100) patients in lbn-Albeetar and Baghdad Teaching Hospital. The result of the study indicated the following; 45% of patients with age group (41-50) were more exposed to the disease and there is no significant difference was seen in the level of education, Martial status, weight and height. The result shows that there are significant difference in risk factors like hypertension, cholesterol level in blood and diabetes. When analyzed by T.test at level of P < 0.01 and there are significant difference in smoki

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Publication Date
Sun Sep 05 2010
Journal Name
Baghdad Science Journal
Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial ac

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Publication Date
Fri Jan 20 2023
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of the Crude Terpenoids Compounds of Melia Azedrach Leaves and Fruits on Some Biological Aspects of Whitefly Bemisia tabaci

This study was carried out to determine the effects of crude terpenoid compound extracts of Melia azedarach L. leaves and fruits on some biological aspects of whitefly Bemisia tabaci (Genn.) under laboratory conditions of 25 ± 2°C and relative humidity of 65-70%. The results indicated a significant effect on eggs and immature stages when treated with 0.5, 1, 1.5, and 2 mg/ml of leaves and fruits. The mortality rate increased with concentration. It reached 98.68, 100, 100, 95.70, 86.30, and 42.68% for eggs, 1st, 2nd, and 3rd nymphal instars, pupa, and adults, respectively, at a concentration of 2 mg/ml for leaves extract. At the same time, it reached 94.86, 100,100, 100, 87.89, and 44.90% for eggs, 1st, 2nd, and 3r

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Publication Date
Sun Mar 01 2009
Journal Name
Baghdad Science Journal
Purification and Characterization &#946; - lactamase produce from local isolate Klebsiella pneumonia

Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration.Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ? C .During storage the enzyme retained 72% at -20 ? C and retained 25% of the activity at the same period at 4 ? C.

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Publication Date
Sun Jul 24 2022
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Assessment of Smoking Dependence by FAGERSTRŐM Test and its Association with Some Comorbidities and Educational level

Background: Nicotine addiction is the inevitable result for many smokers. Regardless of control and intervention strategies for smoking cessation, it is important to determine the nicotine dependence score for each smoker. 

Objectives: To assess the nicotine dependence by FAGERSTRŐM Test for nicotine dependence (FTND score) and to find out the association of nicotine dependence with some smoking-related comorbidities and with the level of education.

Patients and Methods: This is a cross-sectional study on a total of 200 adult smokers who were attending different departments in Al-Imamain Al-Kadhimain Medical City during the period from April 2019 to October 2019. Demograp

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Publication Date
Wed Apr 26 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Adsorptive Removal Of Congo Red From Aqueous Solution By Local Chaff Surface: Thermodynamics And Kinetics Studies

This study is concerned with the adsorption of Congo red from solution on the surface of Chaff. The adsorption isotherm is of L-curve type according to Giles classification and the experimental data were best fitted to Langmuir isotherm model. The adsorption phenomenon was examined as a function of temperature (25, 40, 55 oC). The extent of adsorption of Congo red on Chaff was found to increase with the increase of temperature (endothermic process). The basic thermodynamic functions have also been calculated. The effect of contact time was investigated and found that the adsorption process of dye on Chaff surface reached complete equilibrium within 90 min. The maximum uptake of Congo red by Chaff was found to be 92.9% at 25oC. The kineti

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Publication Date
Tue May 12 2020
Journal Name
International Journal Of Pharmaceutical Research
Synthesis and Biological Activity of New Imidazaldinone Derivatives

A research include of synthesized five member ring, which has been synthesized by condensation of 2-(1-(4- hydroxyphenyl) ethylidene)hydrazine-1-carbothioamide[I] with α-chloro ethylacetate in sodium acetate (fused) to form ethyl 2-(4-(1-((4-oxo-2-thioxopyrrolidin-3-ylidene)hydrazono) ethyl)phenoxy)acetate [II]. After that reacted [II]with hydrazine hydrate to resulting compound [III]. Compound [III] was refluxed with 4-aminobenzoicacid in excess of phosphorus oxy chloride give compound [IV],the later compound [IV] interact with acetyl acetone or ethylacetoacetate give to pyrazole and pyrazoline derivatives[V],[VI]. While when reacted with different aromatic aldehydes and one ketone consistence Schiff base derivatives [VII]a-e . The FTIR a

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Publication Date
Mon Jun 01 2020
Journal Name
Journal Of Pharmaceutical Sciences And Research
Synthesis and Biological Activity of New Imidazaldinone Derivatives

A research include of synthesized five member ring, which has been synthesized by condensation of 2-(1-(4- hydroxyphenyl) ethylidene)hydrazine-1-carbothioamide[I] with α-chloro ethylacetate in sodium acetate (fused) to form ethyl 2-(4-(1-((4-oxo-2-thioxopyrrolidin-3-ylidene)hydrazono) ethyl)phenoxy)acetate [II]. After that reacted [II]with hydrazine hydrate to resulting compound [III]. Compound [III] was refluxed with 4-aminobenzoicacid in excess of phosphorus oxy chloride give compound [IV],the later compound [IV] interact with acetyl acetone or ethylacetoacetate give to pyrazole and pyrazoline derivatives[V],[VI]. While when reacted with different aromatic aldehydes and one ketone consistence Schiff base derivatives [VII]a-e . The FTIR a

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