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Preparation of Nanoliposomes by Size Exclusion Chromatography to Entrap Soluble Antigens from Leishmania Donovani
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In this study, we investigated the ability of nanoliposomes preparation, as a nanoadjuvant, to entrap soluble Leismania donovani antigens (SLAs) and release in vitro. The parasite reactivation was carried out when inoculated into Rosewell park memorial institute media (RPMI) and incubated at 23 °C for 4 days. L. donovani promastigote inoculum (104 cell / ml) of 4 days was used to inoculate modified medium of Saline - Neopeptone and Blood agar 9 (SNB 9) to produce promastigote mass. SLAs were extracted from the promastigotes ghost membrane after fourth passages of subculturing in SNB. The membrane pellet obtained was suspended in 5 mM Tris buffer (pH 7.6) and sonicated three times at 4 °C and entrapped in freshly prepared nanoliposomes. Lipids mixture of 4mM Phosphatidylcholine, 2.2 mM Cholesterol and 0.55 mM Phosphatidylethanolamine in a ratio of 7:2:1 were used to prepare nanoliposome. Physio-chemical characterizations of prepared nanoliposomes was performed by using Scanning Electron Microscope (SEM) , Atomic Force Microscope (AFM) and Zeta Potential assays to determine the size, morphology and charge. The efficiency of freshly prepared nanoliposoms to entrap SLAs was determined by measuring the nanoliposome efficiency entrapment (EE). The percentage of EE was 50 and 27.5 of SLAs entrapped nanoliposomes prepared by Sephadex G25 and Sephadex G75, respectively. Moreover, stability of SLAs entrapped nanoliposomes was examined at 4 and 37 °C as a storage temperature.

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Publication Date
Mon Jan 30 2023
Journal Name
Iraqi Journal Of Science
Texture Features of Grey Level Size Zone Matrix for Breast Cancer Detection
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    The texture analysis of cancer cells leads to a procedure to distinguish spatial differences within an image and extract essential information. This study used two test tumours images to determine cancer type, location, and geometric characteristics (area, size, dimensions, radius, etc.). The suggested algorithm was designed to detect and distinguish breast cancer using the segmentation-based threshold technique. The method of texture analysis Grey Level Size Zone method was used to extract 11 features: Small Zone Emphasis, Large Zone Emphasis, Low Grey Level Zone Emphasis, High Grey Level Zone Emphasis, Small Zone Low Grey Level Emphasis, Small Zone High Grey Level Emphasis, Large Zone Low Grey Level Emphasis, Large Zone High Gre

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Formation Silver Nanoparticles of Different Size Using Different Reductants with AgNO3 Solution
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In this study, silver nanoparticles (AgNPs) are synthesized using different chemical routes to obtain different sizes and shapes of nanoparticles by colloid chemistry with using stabilizing and reducing agent, which make them interesting for variety of physical applications. The morphology and structure of the synthesized AgNPs were characterized by UV-VIS spectra, Scanning Electron Microscopy (SEM) and Zeta potential to demonstrate that different sizes and shapes can by synthesized by different reductants in the presence of various stabilizing agents.

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Publication Date
Sun Apr 30 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Modeling of the Effect of Grain Size on Final Stage of Sintering Al2O3
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  This research studies the effect of grain size for the final stage in sintering Al2O3 .The experimental results for α- Al2O3 powder are modeled using ( L2-Regression ) technique in  order to study the effect grain size distribution on densification rate using four kinds for the initial particle size which were ( 1.44 , 2.54 , 0.7-2.54 ,1.15-3.53 ) µm , and for sintering time  (0-241) min. The mathematical simulation for grain size changing shows that the densification rates boots up as the grain size goes lower, this was due to the increase of contact area between the grains.

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Publication Date
Wed Sep 01 2010
Journal Name
The International Journal Of Biochemistry & Cell Biology
A plate-based assay system for analyses and screening of the Leishmania major inositol phosphorylceramide synthase
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Sphingolipids are key components of eukaryotic membranes, particularly the plasma membrane. The biosynthetic pathway for the formation of these lipid species is largely conserved. However, in contrast to mammals, which produce sphingomyelin, organisms such as the pathogenic fungi and protozoa synthesize inositol phosphorylceramide (IPC) as the primary phosphosphingolipid. The key step involves the reaction of ceramide and phosphatidylinositol catalysed by IPC synthase, an essential enzyme with no mammalian equivalent encoded by the AUR1 gene in yeast and recently identified functional orthologues in the pathogenic kinetoplastid protozoa. As such this enzyme represents a promising target for novel anti-fungal and anti-protozoal drugs. Given

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Publication Date
Wed Nov 01 2017
Journal Name
Research Journal Of Pharmaceutical, Biological And Chemical Sciences
Preparation, Spectral studies and Biological Efficiency of Metal (II) Mixed Ligand Complexes Schiff Base derived from (Benzaldehyde and OAminoaniline) with 8-Hydroxyquinoline
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fication of benzaldehyde (C6H5CHO) and O- amino aniline O-C6H4(NH2)2 in ethanol with 8- Hydroxyquinoline (8HQ) . Formed compounds were acquired of 1:1:2 molar proportion reactions for metal ions and ligands (L) and 2(8HQ) during reaction for MCl2 .nH2O salt products complexes conformable into the forms [M(L)(8HQ)2] ,where M = Mn(II),Co(II) and Ni(II). Whole the compounds were identified during the basis of their; FT-IR and U.V spectrum, melting point, molar conduct, identify of the percentage from the metal at the complexes via flame (AAS), C, H and N content of the Schiff base (L) and metal complexes were analysis and magnetic susceptibility menstruations. A hexagonal coordinated metal complexes were proposed to the separated complexes of

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Publication Date
Mon Oct 01 2018
Journal Name
Journal Of Economics And Administrative Sciences
Comparison of the statistical methods used to Forecast the size of the Iraqi GDP for the two sectors (public and private) for the period (2025-2016)
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Gross domestic product (GDP) is an important measure of the size of the economy's production. Economists use this term to determine the extent of decline and growth in the economies of countries. It is also used to determine the order of countries and compare them to each other. The research aims at describing and analyzing the GDP during the period from 1980 to 2015 and for the public and private sectors and then forecasting GDP in subsequent years until 2025. To achieve this goal, two methods were used: linear and nonlinear regression. The second method in the time series analysis of the Box-Jenkins models and the using of statistical package (Minitab17), (GRETLW32)) to extract the results, and then comparing the two methods, T

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Publication Date
Fri Jan 01 2021
Journal Name
Aip Conference Proceedings
Preparation and characterization of biomass-alumina composite as adsorbent for safranine-o dye from aqueous solution at different temperatures
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Publication Date
Thu Feb 07 2019
Journal Name
Iraqi Journal Of Laser
Investigation of Size-dependent Nonlinearity of Ag Nano-Fluids using Self-defocusing Technique
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In this work, the effect of different particle size on the nonlinear optical properties of silver nanoparticles in de-ionized water was studied. The experimental observation of the far field diffraction patterns by CCD camera in two and three dimensions. The maximum change of nonlinear refractive index and the relative phase shift were calculated. The self-defocusing technique was used with a continuous-wave radiation from DPSS Blue laser .The wavelength is 473 nm with an output power of 270 mW. All the Ag colloids samples containing the sizes 15, 30, 50, and 70 nm of silver nanoparticles used in the study were chemically prepared. It was found that the nonlinear refractive index is a particle size dependent and of the order of 10-7 cm2/

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Publication Date
Tue Jan 18 2022
Journal Name
Materials Science Forum
The Crystallite Size Dependence of the Transmittance Property of Vanadium Dioxide Nano Films
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Bruggeman's symmetric effective-medium model of vanadium oxide is introduced, in which the transmittance was studied because of its importance in the subject of smart windows, it was studied from ( 5 nm-1000 nm) for each of the regions of the electromagnetic spectrum, the ultraviolet and visible region, and the near and medium sub-regions of the infrared and the results showed that the importance of studying the transmittance of vanadium oxide as a good candidate For this kind of industries. Our results showed that the small sizes of the material guarantee an almost constant and high transmittance to the visible region; this is due to the agreement of the direction of the dipoles in the material with the direction of the internal el

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Preparation of phenolic resin complexes
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in this worl three types of complexed phenolic resins were prepared using various additives such as and improving the aim of this work higher mechanical properties this work is done

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