This study aimed for isolation and identification of Candida glabrata from specimens of blood collected from national center of hematology /Al - mustansiriya University of immunocompromised patients infection of candidemia after diagnosis by doctor. Results showed the morphological features on many media SDA and Corn meal and differential CHROMagar. Colonies appear small and pink pale to dark. Microscopic exam of show that C. glabrata not form pseudohyphae and not produce germ tubes, the cell size 2 - 3 microns, on the other hand, Biochemical test of C. glabrata have high ability for fermentation of glucose within three hours and trehalose given false positive within two hrs., and the Urease test given negative result, on the other hand , The results indicated that the DNA of isolated strains was extracted successfully. The results of PCR amplification which was performed on that DNA extracted from all isolates were confirmed by the electrophoresis analysis. The primer amplified of the ribosomal DNA in all strains tested, resulting in amplified products of approximately 610 bp in C. glabrata.
The present study aims to evaluate levels of calcium regulating hormones and some biochemical parameters in a sample of growth hormone (GH) deficient patients. Seventy five GH deficient patients and twenty healthy subjects used as a control group have been involved in this study during their attendance at the National Diabetic Centerfor Treatment and Research /Al-Mustansiriya University. The studied subjects were in an age range of 3-15 years. Blood samples were collected from the studied subjects to determine levels of basal GH,GH2. and GH3 after 60 mins. and 90mins. of provocation with clonidine. The study also included the measurement of the levels of insulin like growth factor (IGF-1); calcium regulating hormones [parathyroid hormone
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(2)
(1)
This study was conducted to evaluate the prevalence rate of
toxoplasmosis among 294 rheumatoid arthritis (RA) patients treated with
methotrexate (MTX), 50 RA patients without treatment and 50 samples as
healthy control. Blood samples were collected and the presence of T.gondii
IgG and IgM antibodies was determined by using Enzyme linked
immunosorbent assay (ELISA). Tumor necrosis factor alpha (TNF-α) was
also estimated in serum of all subjects by using ELISA method too. The
seroprevalence of toxoplasmosis IgM and IgG in RA+MTX was
60(20.408%), and 98(33.33%), in RA patients 4(8%), and 18(36%) while,
it was 2(24%), 6(12%) in healthy group. Tumor necrosis factor alpha
(TNF-α) was also estimated in serum of a
This study was conducted to determine the relationship between two most common diseases in Iraqis patients , which are Diabetic mellitus (DM) and Rheumatoid Arthritis (RA); seeking rheumatoid factor in hyperglycemic sera. The results revealed that ; 62.5% of hyperglycemic (HG) patients had positive rheumatoid factor (RF) . No difference in number between both gender of HG patients (20 males and 20 females ) , RF reaction was nearly similar in males and females of HG patients ( 12 &13 respectively ) . Only 40% out of patient controls had positive RF . None of the apparently healthy subjects had positive RF .
Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chromatography using DEAE- cell
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(5)
Celiac disease (CD) is the most common genetically - based disease in correlation with food intolerance. The aim of this study is to measure the activity of ALT enzyme and purify enzyme from sera women with celiac disease. Alanine aminotransferase (ALT) activity has been assayed in (30) women serum samples with celiac disease, age range between (20-40) year and (30) serum of healthy women as control group, age range between (22-38) year. In the present study, the mean value of ALT activity was significantly higher in patients with celiac disease than healthy group (p<0.01). The ALT enzyme was partial purified from sera women with celiac disease by dialysis, gel filtration using Sephadex G- 50 and ion exchange chr
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(5)
One of the most common forms of diabetes is Type-2 that occurs due to the failure of cells in recognizing and responding to insulin if not accurately treated. The aim of this work is to evaluate the relations of thyroid hormones, vitamins, and lipid peroxidation with the glycemic index in patients experiencing Type-2 diabetes. Some tests of biochemical parameters and vitamins were conducted on 35 patients experiencing Diabetes Mellitus (DM) and 35 healthy subjects. The results indicated the increase in the levels of MDA (3.86 ± 0.97 µmol/L), HbA1c (8.27 ± 1.66 %), FBS (198.34 ± 32.41 mg/dl) and TSH (5.67 ±0.34 mIU/L) in the blood of diabetic subjects in comparison to the controls at a P value lower than 0.05. These incr
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(6)
The study involved 120 women, who were distributed into two groups of breast tumor patients (30 malignant and 30 benign) and a group of controls (60 women). The patients were referred to the Center for Early Detection of Breast Tumor at Al-Alwayia Hospital for Gynecology and Obstetrics (Baghdad) during the period June-December 2011. They were investigated for the frequency of ABO blood group phenotypes, menopausal status, oral contraceptive use, body mass index and family history of breast cancer or other cancers. The results demonstrated that 60.0% of malignant cases clustered after the age 50 years, while it was 20.0% in benign cases. Fifty percent of malignant breast tumor patients reached menopause, while in benign cases, the corresp
... Show MoreCopper (Cu) Zinc (Zn) and Magnesium (Mg) in serum, RBC, urine and dialyzate fluids were
studied in 39 patients, who have been undergoing chronic haemodialysis treatment. They were
divided in to polyuric , oliguric and anuric depending on their urinary output. Elevated serum and
RBC Mg was observed before dialysis, while decreased serum and RBC level was noticed except
serum Mg of polyuric patients. Before dialysis elevated serum and RBC Zn were observed. While
after dialysis these parameters were increased. Normal RBC Cu value before dialysis was observed.
While low serum Cu was noticed. After dialysis serum Cu showed raised value, while RBC level
decreased in oliguric and increased in polyuric patients. Zn / Cu rati
Endocrine Abnormalities in β-thalassemia major are common disturbing
complications, that need prompt management. Importance of this work was to
evaluate the some biochemical parameters and endocrine hormones related to
the pubertal maturity and fertility status in cases with thalassemia. A sixty patients
[38 males and 22 females] with β - thalassemia major against 30 healthy subjects
[17 males and 13 females] were enrolle . The Blood levels of, Leptin, Vitamin D,
thyroid function test, parathyroid hormone, ghrelin, and sex hormones were
determined in the blood. Body Mass Index (BMI) was also evaluate. Results
showed that besides lower BMI, all hormones assessed were significantly lower in
thalassemia β-major
Total protein and total fucose were determined in sera of thyroid
disorder patients.
Sera of (40) diagnosed by consultant hyperthyroidism, and 40 hypothyroidism were analyzed for the above parameter for control, sera of (40) normal individuals were used.
They were healthy with no appearing disorder results analysis revealed no significant differences (P<0.05) in the (mean ±SD) of total protein values in sera of hyper and hypothyroidism were compared
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