Rosemary is a well-known aromatic and medicinal plant used to treat various ailments. This study evaluated Rosmarinus officinalis essential oil for its phytochemical and antibacterial properties. The essential oil was analysed by using a gas chromatography-mass Spectrometry (GC-MS) that revealed the common chemicals containing verbenone 36.20% and 1,8-cineol (Eucalyptol) 12.14%. Extracted essential oils were tested for antibacterial activity against vancomycin intermediate Staphylococcus aureus (VISA), a strain of bacteria obtained locally from bacteremia patients. Three isolates were found to be VISA positive using the E-test (strips) and the population analysis profile method (PAP). VISA showed lower resistance to the essential oils (MIC: Minimum inhibitory concentration, MIC = 1.5 mg/ml). Overall, the genetic influence of VISA was evaluated by looking at the hld gene expression after being isolated from blood samples and RNA extraction for qRT-PCR to determine hld gene expression. R. officinalis gene expression was analysed and calculated by using real-time PCR of RNA which revealed that it had decreased.
Thirty swabes of medical implants were collected from Al-Yarmouk's hospital which were cultured on manitole agar to isolate Staphelococcus aureus . Only four samples gave positive results with this media. It was used ten types of antibiotics to test the sensitivity of this bacterium against them. All isolates of S. aureus were recorded as multidrug resistant and were considered as MRSA. One pledge alternative therapy is the utilize of certain pure bacterocin MIC (32.5 to 62.5 μg/ml) and it was compared with vancomycin (200-400 μg/ml) with average of (8 – 15) mm diameter of inhibition zones recpectively. The first reduction of biofilm formation ability has been proved in catheters when treatedby pure bacterocin. The test shows the highes
... Show MoreThe study included the extraction of volatile oil from Mentha piperita which was 1.3 % in the leaves and flowers . Volatile oil of the Mentha piperita leaves had special aromatic odour, pale yellow color, slightly pungent taste . The specific gravity and refractive index were (0.9794) and ( 1.464) respectively. The inhibition activity of the Mentha piperita Volatile oil extracts were studied on some pathogenic microorganisms like Staphylococcus aureus, Salmonella typhi, Escherichia coli, Proteus sp, and Klebsiella pneumoniae . The result showed that the volatile oil had an inhibition effect on the growth of all microorganisms, and it gave the higher inhibition effect on the growth of S. aureus in which the inhibition zone reached to 2
... Show MoreNormally, bacteria exposed to antibiotics at sub minimal inhibitory concentrations (MIC) inside the host. Therefore, the current study aimed to comprehend the association among hemolysins, biofilm, as well as gentamicin resistance in local MRSA isolates. Around 35 Staphylococcus aureus locally isolated from different clinical specimens were employed in this study. Methicillin resistance was detected via cefoxitin disk diffusion and mecA amplification methods. MIC of gentamicin was estimated by broth microdilution method. Hemolysin genes involving hla, hlb, hld, and hlg were determined using multiplex polymerase chain reaction (PCR) technique. Microtiter plate method was employed for biofilm assessment in the presence and absence of gentamic
... Show MoreThe study aimed to find an association between Type two diabetes mellitus (T2DM) patients, obesity and the rate of nasal carriage of Staphylococcus aureus (NCSA) producer of TSST-1 in patients with T2DM compared with non-diabetic control groups. T2DM patients and control subjects were selected from outpatient of "The Specialist Center for Diseases of Endocrine and Diabetes" in Baghdad. The subjects were divided into 4 groups: Group I included 21 obese T2DM patients; Group II included 20 lean T2DM patients; Group III included 20 obese as control group and Group IV included 21 lean as control group. The study included sample with size (n= 82), male and female, with the ages ranged from 35 to 75 years, and the patients were not on any kind
... Show MoreThis study includes isolation, purification, and identification of algae from the canal around Baghdad university Al-jadriah. Four unialgal cultures were obtained. These algal cultures included 3 species of cyanophyta ( Nostoc carneum, Westillopesis prolifica, Chroococcus turgidus), 1 species of chlorophyta (Chlorella vulgaris) . Different plants belonging to different families were collected and extracted for their oils which were Ricinus communis and Sesamum indicum (seeds), Matricaria chamomilla (flowers) .However, antialgal activity of the extracted oils were evaluated the isolated algae with 7 concentrations (0.09, 0.3, 0.5, 1, 10, 20 , 30) % using the agar wells diffusion method. Results showed that R. communis oil was more effecti
... Show MoreSilver nanoparticles synthesized from aqueoes extract for mushroom pleurotus ostreatus. Silver nanoparticles were showing good antibacterial activity. The antibacterial activity conducted against different pathogenic bacteria such as Serratia marcescens, Pseudomonas fluorescens, Listeria, Methicillin-resistant Staphylococcus aureus (MARSA), Proteus mirabilis, Proteus vulgaris and Klebsiella pneumoniae. The maximum inhibition zone was observed against S. marcesance. However, the lowest inhibition zone was found against P. fluorescens.
During 2011, 1900 clinical specimens and 240 hospital environment specimens were collected from four hospitals in Baghdad. 128 isolates of Acinetobacter baumannii were obtained from clinical and environmental specimens in a percentage of 6.05% and 5.42%, respectively. The highest percentage of isolation, 83.62% was of sputum specimens and lower percentage of burns specimens 5.22%. The lowest incidence was of age range (71-80) years old group whereas the highest incidence was of age range (31-40) years old group. Also we found that the incidence was higher in males (66.96%) than that of females (33.04%) and the frequency of positive A. baumannii isolates was higher in intensive care units (ICUs). Results revealed eleven different resistot
... Show MoreThree beach soils in Lagos, Nigeria were screened for the presence of antibiotic producing fungi against 8 test pathogenic bacteria & fungi. The physiochemical parameters of the soils were determined following standard procedures. Soil plate dilution method was employed for isolation of marine fungi and they were identified based on cultural and microscopic characteristics. Primary screening of isolated fungi for antibiotic potential was determined by perpendicular streak method against known pathogenic test organisms (Escherichia coli, Saphylococcus aureus, Klebsiella pnuemoniae, Pseudomonas aeruginosa, Candida albicans, Saccharomyces cerevisiae,Aspergillus niger and Aspergillus fumigatus). Further sreening of the product o
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