MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post-transcriptionally. Transitional cell carcinoma(TCC) of the bladder Cancer (C) account 95 percent of bladder malignancies, with males having a greater prevalence than females. The current study sought to determine whether there is a link between miRNA-29c, miRNA-125, miRNA-141, miRNA-145 and miRNA205 expression levels and TCC/BC risk in Iraqi bladder cancer patients. In the current prospective cross-sectional investigation, 149 samples were collected (95 urine and 54 tissue biopsies). From November 2018 to August 2019, 37/95 urine samples were randomly taken from healthy persons. Total RNA was extracted from tissue and urine samples, and then converted to cDNA via reverse transcription. Quantitate Real-Time-PCR was done using specific primers for quantification of gene expression level of the studied miRNAs. The results showed that 32/49 (65%) patients had non-muscles invasive bladder cancer (stage T1), while 17/49 (35%) patients had muscles invasive bladder cancer (stage T2-T4). Fold change in miRNA-125 expression level showed highly significant differences between non-muscles invasive bladder cancer (T1) and muscles invasion bladder cancer (T2-T4) in both urine and tissue samples biopsies using Chi-square test at p ≤ 0.01. Whereas miR-29c, miR-141, miR-145 and miR-205 showed no significant differences at p > 0.05 between muscles invasive bladder cancer (T1) and muscles invasive bladder cancer (T2-T4) in both urine and tissue biopsies samples. Thus, miR-125 can be associated with the development of invasive stages of TCC-BC as there is an increase in miR-125 expression level in the urine of patients during the final stage of cancer.Hence, this gene could be considered a good predictor in advanced stages of bladder cancer.
Background: Polycystic ovarian syndrome is one of the common gynaecological diseases encountered nowadays in the gynaecological clinic. Many criteria and diagnostic test had been evolved to be used with different classifications methods.Objectives: The present study aimed to measure the anti-mullerian hormone levels in serum of the women with Polycystic Ovary Syndrome and to test the possibility that if it can be used as a marker for diagnosis of polycystic ovary syndrome patients.Methods: A cross sectional study that had been conductedat Kamal AL-Samaraee Hospital, AL-Suwayrah Hospital andAl-Elwiya Maternity Teaching Hospital during the periodfrom July, 1st, 2013 – Jan. 1st, 2014. Where forty women withPolycystic ovarian syndrome (wit
... Show MoreBackground: Oral carcinogenesis is a molecular and histological multistage process featuring genetic and phenotypic markers for each stage, which involves enhanced function of several oncogenes and/or the deactivation of tumor suppressor genes, resulting in the loss of cell cycle checkpoints. The progression towards malignancy includes sequential histopathological alterations ranging from hyperplasia through dysplasia to carcinoma in situ and invasive carcinoma. The p16 gene produces p16 protein, which in turn inhibits phosphorylation of retinoblastoma, p16 play a significant role in early carcinogenesis. Human papillomavirus is a well established heterogeneous virus and plays an important role in oral cancers. The aims of the study were to
... Show MoreIn this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay
tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients
hospitalized with signs and symptoms of an upper respiratory tract infection in Central
Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus
infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was
done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell
line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples.
Background: Breast cancer is the most common
malignancy affecting females worldwide. The association
of Epstein-Barr virus (EBV) with this cancer is a longstanding
interest to this field.
Aim: to investigate the presence of EBV in breast tumor
tissue in relation to age.
Patients and Methods: Paraffin-embedded tissue blocks
from 45 female patients with breast tumors (ranged in age
from 28 to 85 years) were retrieved. The cases were
grouped into two categories: group (A): included 30 cases
with breast carcinoma and group (B): included 15 cases
with benign breast diseases as a control group .The
expression of EBV protein was examined
immunohistochemically.
Results: Twelve (40%) of the 30 breast canc
Ninety eight specimens were collected from patients referring different hospitals in Baghdad in period from August to November in 2012. Specimens including (swabs from (Sputum, burn, wound, urine, ear, and eye).Sixty six isolates were identified as Pseudomonas aeruginosa. The isolates were identified according to morphological, cultural, biochemical characteristics and API 20E test. 90% of P. aeruginosa isolates produced pyocyanin pigment on King A medium in different amounts, whereas other isolates were produced other types of pigments such as (pyoverdine-yellow, pyorubin-red, and pyomelanin-black) on King B medium and also in different amounts.Quantitative assay of pyocyanin production was conducted. The results were shown that the iso
... Show Moreيعتبر "تاج الأشواك" أو نبات شوكة المسيح، وهو من نباتات الزينة الطبية ، ينتمي إلى جنس يوفوربيا. E. milii يحتوي كميات وفيرة من المركبات الفينولية ، التربينات، الستيرويدات والقلويدات. كانت الأهداف الرئيسية لهذه الدراسة هي فحص مستخلصات الفلافونويد والنانو فلافونويد ضد نوعين من خطوط الخلايا السرطانية. تم تصنيع مركبات الفلافونويد النانوية عن طريق تفاعل مركب الكيتوسان والماليك اسد. تم تحليل مركبات الفلافونويد ال
... Show MorePsoriasis is a chronic inflammatory skin disease that is closely related to the oxidative stress state of the body. The current study focused on some immunological indicators directly related to inflammation (Procalcitonin, and lysozyme) with the aim of investigating their potential as a prognostic factor for psoriasis. This study included 150 samples, including 90 patients with psoriasis and 40 people who appeared to be healthy, and was conducted from November 2021 to April 2022. The results showed a significant increase in the level of procalcitonin in patients with psoriasis compared to its level in the control group, while it was noted that the increase in the level of the other indicators was not significant, according to the statis
... Show MoreMany studies of the relationship between COVID-19 and different factors have been conducted since the beginning of the corona pandemic. The relationship between COVID-19 and different biomarkers including ABO blood groups, D-dimer, Ferritin and CRP, was examined. Six hundred (600) patients, were included in this trial among them, 324 (56%) females and the rest 276 (46%) were males. The frequencies of blood types A, B, AB, and O were 25.33, 38.00, 31.33, and 5.33%, respectively, in the case group. Association analysis between the ABO blood group and D-dimer, Ferritin and CRP of COVID-19 patients indicated that there was a statistically significant difference for Ferritin (P≤0.01), but no-significant differences for both D-dimer and CRP.
... Show MoreBackground: Excision repair cross-complementing group 2 gene (ERCC2) polymorphisms have been linked as being a risk factor for colorectal cancer (CRC) emergence. However, data from several studies are contradictory. To validate genetic biomarkers of the CRC; the impact of the following ERCC2 polymorphism (rs1799793 and rs238406) was examined on CRC susceptibility among sample of Iraqi population. Methods: A total of 126 subjects were enrolled in this case control study; 78 CRC patients and 48 apparently healthy individuals who are age, gender, smoking status and BMI matched. Polymerase chain reaction (PCR) was used for genotyping, followed by sequencing then the association between genetic polymorphisms and CRC risk was investigate
... Show MoreDiabetic kidney disease is an illness of the glomerulus that interferes with the glomerular filtration barrier (GFB), which is worked to enable kidney to selective purification of water and solutes in addition to limiting the movement of large macromolecules such as albumin. In the glomerular endothelium, mesangial cells, foot cells, and the brush border of the proximal tubules, ACE-2 is expressed and that the kidneys represent the highest-expressing region of this enzyme. Thus, the current study aimed to evaluate ACE-2 level in this case compared to healthy condition. The study Conducted with 120 male and female ranging in age (30-65) years old. Ninety patients with type 2 diabetes subdivided into three groups on the basis of A
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